Limits...
Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors.

Shenje LT, Andersen P, Uosaki H, Fernandez L, Rainer PP, Cho GS, Lee DI, Zhong W, Harvey RP, Kass DA, Kwon C - Elife (2014)

Bottom Line: Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development, yet the intrinsic factors and environment governing these processes remain unclear.With histological, flow cytometric and functional analyses, we find that CPCs remain undifferentiated and expansive in the PA2, but differentiate into cardiac cells as they exit the arch.Tracing of Nb- and Nbl-deficient CPCs by lineage-specific mosaicism reveals that the CPCs normally populate in the PA2, but lose their expansion potential in the PA2.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, United States The Knight Cardiovascular Institute, Oregon Health & Science Universtiy, Portland, United States Institute for Cell Engineering, Johns Hopkins University, Baltimore, United States.

Show MeSH

Related in: MedlinePlus

Numb/Numbl DKO cells are normally specified into OT cells.(A–C) Confocal images of PA2 sections of E9.0 chimera, immunostained with RFP and Isl1 (A), RFP and Mef2c (B) or RFP, Isl1 and Mef2c (C) antibodies. RFP+ Isl1+ Mef2c+ cells are outlined in white (C). Dapi (blue) was used to counterstain the nuclei. Scale bars, 50 mm. ot, outflow tract; pa, pharyngeal arch.DOI:http://dx.doi.org/10.7554/eLife.02164.020
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4007206&req=5

fig5s1: Numb/Numbl DKO cells are normally specified into OT cells.(A–C) Confocal images of PA2 sections of E9.0 chimera, immunostained with RFP and Isl1 (A), RFP and Mef2c (B) or RFP, Isl1 and Mef2c (C) antibodies. RFP+ Isl1+ Mef2c+ cells are outlined in white (C). Dapi (blue) was used to counterstain the nuclei. Scale bars, 50 mm. ot, outflow tract; pa, pharyngeal arch.DOI:http://dx.doi.org/10.7554/eLife.02164.020

Mentions: The phenotype of DKO chimeras depended on the contribution of the donor RFP+ cells. Major donor contribution caused a phenotype similar to DKO embryos (12.5% 2/16, Figure 5F,G). In most cases (87.5% 14/16), the chimeras were indistinguishable from wild-type embryos (Figure 5H,I). Donor RFP+ cells were normally populated in the PA2 and contributed to the OT, cardiomyocytes and endocardial cells (Figure 5J–M, Figure 5—figure supplement 1), indicating deletion of Numb and Numbl did not affect the migration or cardiac differentiation of CPCs.


Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors.

Shenje LT, Andersen P, Uosaki H, Fernandez L, Rainer PP, Cho GS, Lee DI, Zhong W, Harvey RP, Kass DA, Kwon C - Elife (2014)

Numb/Numbl DKO cells are normally specified into OT cells.(A–C) Confocal images of PA2 sections of E9.0 chimera, immunostained with RFP and Isl1 (A), RFP and Mef2c (B) or RFP, Isl1 and Mef2c (C) antibodies. RFP+ Isl1+ Mef2c+ cells are outlined in white (C). Dapi (blue) was used to counterstain the nuclei. Scale bars, 50 mm. ot, outflow tract; pa, pharyngeal arch.DOI:http://dx.doi.org/10.7554/eLife.02164.020
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4007206&req=5

fig5s1: Numb/Numbl DKO cells are normally specified into OT cells.(A–C) Confocal images of PA2 sections of E9.0 chimera, immunostained with RFP and Isl1 (A), RFP and Mef2c (B) or RFP, Isl1 and Mef2c (C) antibodies. RFP+ Isl1+ Mef2c+ cells are outlined in white (C). Dapi (blue) was used to counterstain the nuclei. Scale bars, 50 mm. ot, outflow tract; pa, pharyngeal arch.DOI:http://dx.doi.org/10.7554/eLife.02164.020
Mentions: The phenotype of DKO chimeras depended on the contribution of the donor RFP+ cells. Major donor contribution caused a phenotype similar to DKO embryos (12.5% 2/16, Figure 5F,G). In most cases (87.5% 14/16), the chimeras were indistinguishable from wild-type embryos (Figure 5H,I). Donor RFP+ cells were normally populated in the PA2 and contributed to the OT, cardiomyocytes and endocardial cells (Figure 5J–M, Figure 5—figure supplement 1), indicating deletion of Numb and Numbl did not affect the migration or cardiac differentiation of CPCs.

Bottom Line: Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development, yet the intrinsic factors and environment governing these processes remain unclear.With histological, flow cytometric and functional analyses, we find that CPCs remain undifferentiated and expansive in the PA2, but differentiate into cardiac cells as they exit the arch.Tracing of Nb- and Nbl-deficient CPCs by lineage-specific mosaicism reveals that the CPCs normally populate in the PA2, but lose their expansion potential in the PA2.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, United States The Knight Cardiovascular Institute, Oregon Health & Science Universtiy, Portland, United States Institute for Cell Engineering, Johns Hopkins University, Baltimore, United States.

Show MeSH
Related in: MedlinePlus