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Hepatoprotective effect of aged black garlic extract in rodents.

Shin JH, Lee CW, Oh SJ, Yun J, Kang MR, Han SB, Park H, Jung JC, Chung YH, Kang JS - Toxicol Res (2014)

Bottom Line: ABG had no significant effect on body weight increase and plasma lipid profile in HFD-fed mice.However, HFD-induced increase in AST and ALT, but not ALP, was significantly suppressed by ABG treatment.These results demonstrate that ABG has hepatoprotective effects and suggest that ABG supplementation might be a good adjuvant therapy for the management of liver injury.

View Article: PubMed Central - PubMed

Affiliation: Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Cheongwon, Chungbuk, Korea.

ABSTRACT
In this study, we investigated the hepatoprotective effects of aged black garlic (ABG) in rodent models of liver injury. ABG inhibited carbon tetrachloride-induced elevation of aspartate transaminase (AST) and alanine transaminase (ALT), which are markers of hepatocellular damage, in SD rats. D-galactosamineinduced hepatocellular damage was also suppressed by ABG treatment. However, ABG does not affect the elevation of alkaline phosphatase (ALP), a marker of hepatobilliary damage, in rats treated with carbon tetrachloride or D-galactosamine. We also examined the effect of ABG on high-fat diet (HFD)-induced fatty liver and subsequent liver damage. ABG had no significant effect on body weight increase and plasma lipid profile in HFD-fed mice. However, HFD-induced increase in AST and ALT, but not ALP, was significantly suppressed by ABG treatment. These results demonstrate that ABG has hepatoprotective effects and suggest that ABG supplementation might be a good adjuvant therapy for the management of liver injury.

No MeSH data available.


Related in: MedlinePlus

Histopathological analysis of liver. SD rats were pre-treated with vehicle or ABG (200mg/kg) and CCl4 (20% in olive oil, 2ml/kg) was administrated orally to induce liver injury. After 48 hr, livers were removed and fixed in formalin. Liver sections were prepared and analyzed by hematoxylin and eosin staining (magnification ×100).
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Figure 0001: Histopathological analysis of liver. SD rats were pre-treated with vehicle or ABG (200mg/kg) and CCl4 (20% in olive oil, 2ml/kg) was administrated orally to induce liver injury. After 48 hr, livers were removed and fixed in formalin. Liver sections were prepared and analyzed by hematoxylin and eosin staining (magnification ×100).

Mentions: Hepatoprotective effect of ABG on CCl4-induced hepatic injury. To investigate the hepatoprotective effect of ABG, we examined the effect of ABG on CCl4-induced liver damage in SD rats. As shown in Table 1, liver weight was increased significantly by CCl4 administration, which is in consistent with previous reports (20,21). However, treatment of rats with ABG inhibited CCl4-induced increase in liver weight in a concentration-dependent manner, although the effect was not statistically significant (Table 1). The level of AST and ALT, which are known as a marker of hepatocellular damage, was also elevated significantly by CCl4 treatment, and this was dose-dependently suppressed by ABG treatment (Table 1). In addition, the hepatobiliary damage, which is manifested by plasma ALP level, was increased significantly, and concentration-dependent, but statistically insignificant, inhibition of CCl4-induced increase in ALP by ABG was observed (Table 1). Plasma ALB level was not changed in all groups examined in this experiment (Table 1). Histopathological analysis demonstrated that CCl4 induced alterations in hepatocyte morphology and infiltration of immune cells, but not severe necrotic cell death in our experimental condition (Fig. 1). However, the changed induced by CCl4 treatment were suppressed by ABG treatment (Fig. 1).


Hepatoprotective effect of aged black garlic extract in rodents.

Shin JH, Lee CW, Oh SJ, Yun J, Kang MR, Han SB, Park H, Jung JC, Chung YH, Kang JS - Toxicol Res (2014)

Histopathological analysis of liver. SD rats were pre-treated with vehicle or ABG (200mg/kg) and CCl4 (20% in olive oil, 2ml/kg) was administrated orally to induce liver injury. After 48 hr, livers were removed and fixed in formalin. Liver sections were prepared and analyzed by hematoxylin and eosin staining (magnification ×100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4007044&req=5

Figure 0001: Histopathological analysis of liver. SD rats were pre-treated with vehicle or ABG (200mg/kg) and CCl4 (20% in olive oil, 2ml/kg) was administrated orally to induce liver injury. After 48 hr, livers were removed and fixed in formalin. Liver sections were prepared and analyzed by hematoxylin and eosin staining (magnification ×100).
Mentions: Hepatoprotective effect of ABG on CCl4-induced hepatic injury. To investigate the hepatoprotective effect of ABG, we examined the effect of ABG on CCl4-induced liver damage in SD rats. As shown in Table 1, liver weight was increased significantly by CCl4 administration, which is in consistent with previous reports (20,21). However, treatment of rats with ABG inhibited CCl4-induced increase in liver weight in a concentration-dependent manner, although the effect was not statistically significant (Table 1). The level of AST and ALT, which are known as a marker of hepatocellular damage, was also elevated significantly by CCl4 treatment, and this was dose-dependently suppressed by ABG treatment (Table 1). In addition, the hepatobiliary damage, which is manifested by plasma ALP level, was increased significantly, and concentration-dependent, but statistically insignificant, inhibition of CCl4-induced increase in ALP by ABG was observed (Table 1). Plasma ALB level was not changed in all groups examined in this experiment (Table 1). Histopathological analysis demonstrated that CCl4 induced alterations in hepatocyte morphology and infiltration of immune cells, but not severe necrotic cell death in our experimental condition (Fig. 1). However, the changed induced by CCl4 treatment were suppressed by ABG treatment (Fig. 1).

Bottom Line: ABG had no significant effect on body weight increase and plasma lipid profile in HFD-fed mice.However, HFD-induced increase in AST and ALT, but not ALP, was significantly suppressed by ABG treatment.These results demonstrate that ABG has hepatoprotective effects and suggest that ABG supplementation might be a good adjuvant therapy for the management of liver injury.

View Article: PubMed Central - PubMed

Affiliation: Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Cheongwon, Chungbuk, Korea.

ABSTRACT
In this study, we investigated the hepatoprotective effects of aged black garlic (ABG) in rodent models of liver injury. ABG inhibited carbon tetrachloride-induced elevation of aspartate transaminase (AST) and alanine transaminase (ALT), which are markers of hepatocellular damage, in SD rats. D-galactosamineinduced hepatocellular damage was also suppressed by ABG treatment. However, ABG does not affect the elevation of alkaline phosphatase (ALP), a marker of hepatobilliary damage, in rats treated with carbon tetrachloride or D-galactosamine. We also examined the effect of ABG on high-fat diet (HFD)-induced fatty liver and subsequent liver damage. ABG had no significant effect on body weight increase and plasma lipid profile in HFD-fed mice. However, HFD-induced increase in AST and ALT, but not ALP, was significantly suppressed by ABG treatment. These results demonstrate that ABG has hepatoprotective effects and suggest that ABG supplementation might be a good adjuvant therapy for the management of liver injury.

No MeSH data available.


Related in: MedlinePlus