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A Probiotic Preparation Duolac-Gold Ameliorates Dextran Sulphate Sodium-induced Mouse Colitis by Downregulating the Expression of IL-6.

Yoon H, Yoon YS, Kim MS, Chung MJ, Yum DY - Toxicol Res (2014)

Bottom Line: Probiotics are live microorganisms that confer a health benefit on the host.Of these cytokines, the expression of interleukin-6 decreased remarkably after Duolac-Gold treatment.Taken together, these results suggest that Duolac-Gold treatment is effective in IBD healing by regulating IL-6.

View Article: PubMed Central - PubMed

Affiliation: R&D Center, Cellbiotech Co. Ltd., Gimpo, Korea.

ABSTRACT
Probiotics are live microorganisms that confer a health benefit on the host. Duolac-Gold is a mixture of six probiotic bacteria containing three species of Bifidobacteria, two species of Lactobacillus, and Streptococcus thermophilus [corrected]. The aim of this study was to assess the anti-inflammatory effects of Duolac-Gold in an inflammatory bowel disease (IBD) mouse model. IBD was induced by administering 1.5% dextran sulfate sodium (DSS) for 10 days. After induction of DSS-induced colitis, Duolac-Gold was orally administered at three different concentrations. Interestingly, Duolac-Gold treatment accelerated IBD healing, and anti-inflammatory activity was assessed by weight loss, length of the colon, and a microscopic damage score by histology. The expression of inflammatory related cytokines was measured in colon tissues and serum. Of these cytokines, the expression of interleukin-6 decreased remarkably after Duolac-Gold treatment. Taken together, these results suggest that Duolac-Gold treatment is effective in IBD healing by regulating IL-6.

No MeSH data available.


Related in: MedlinePlus

Duolac-Gold decreases pro-inflammatory cytokine IL-6 expression. (A) After mRNA isolation from colon tissues and cDNA synthesis, IL-6 was analyzed by RT-PCR, and normalized to GAPDH. (B) Levels of IL-6 expression in mouse serum, (C) immnohistochemical analysis of IL-6 in colon. a. DSS treated mice colon, b. Duolac gold treated mice colon, Data are mean ± standard deviation; **p < 0.005compared to PC group.
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Figure 0004: Duolac-Gold decreases pro-inflammatory cytokine IL-6 expression. (A) After mRNA isolation from colon tissues and cDNA synthesis, IL-6 was analyzed by RT-PCR, and normalized to GAPDH. (B) Levels of IL-6 expression in mouse serum, (C) immnohistochemical analysis of IL-6 in colon. a. DSS treated mice colon, b. Duolac gold treated mice colon, Data are mean ± standard deviation; **p < 0.005compared to PC group.

Mentions: In a preliminary experiment, the DSS treatment induced production of various pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in colon tissue of mice. In particular, IL-6 levels increased significantly by the DSS treatmentcompared with those in the PBS control mice, suggesting that elevated IL-6 expression lead to inflammation (data not shown). Therefore, to investigate whether IL-6 expression was altered during IBD healing induced byDuolac-Gold, the levels of IL-6 were assayed in colon tissues and serum. IL-6 expression decreased significantly in colon tissues following the Duolac-Gold treatment in comparison with mice that were not treated (Fig. 4A). In addition, IL-6 expression levels also decreased in serum and tissue following the Duolac-Gold treatmen in comparison with mice that were not treated (Fig. 4B and Fig. 4C), suggesting that Duolac-Gold may be regulate IL-6 expression.


A Probiotic Preparation Duolac-Gold Ameliorates Dextran Sulphate Sodium-induced Mouse Colitis by Downregulating the Expression of IL-6.

Yoon H, Yoon YS, Kim MS, Chung MJ, Yum DY - Toxicol Res (2014)

Duolac-Gold decreases pro-inflammatory cytokine IL-6 expression. (A) After mRNA isolation from colon tissues and cDNA synthesis, IL-6 was analyzed by RT-PCR, and normalized to GAPDH. (B) Levels of IL-6 expression in mouse serum, (C) immnohistochemical analysis of IL-6 in colon. a. DSS treated mice colon, b. Duolac gold treated mice colon, Data are mean ± standard deviation; **p < 0.005compared to PC group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4007040&req=5

Figure 0004: Duolac-Gold decreases pro-inflammatory cytokine IL-6 expression. (A) After mRNA isolation from colon tissues and cDNA synthesis, IL-6 was analyzed by RT-PCR, and normalized to GAPDH. (B) Levels of IL-6 expression in mouse serum, (C) immnohistochemical analysis of IL-6 in colon. a. DSS treated mice colon, b. Duolac gold treated mice colon, Data are mean ± standard deviation; **p < 0.005compared to PC group.
Mentions: In a preliminary experiment, the DSS treatment induced production of various pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in colon tissue of mice. In particular, IL-6 levels increased significantly by the DSS treatmentcompared with those in the PBS control mice, suggesting that elevated IL-6 expression lead to inflammation (data not shown). Therefore, to investigate whether IL-6 expression was altered during IBD healing induced byDuolac-Gold, the levels of IL-6 were assayed in colon tissues and serum. IL-6 expression decreased significantly in colon tissues following the Duolac-Gold treatment in comparison with mice that were not treated (Fig. 4A). In addition, IL-6 expression levels also decreased in serum and tissue following the Duolac-Gold treatmen in comparison with mice that were not treated (Fig. 4B and Fig. 4C), suggesting that Duolac-Gold may be regulate IL-6 expression.

Bottom Line: Probiotics are live microorganisms that confer a health benefit on the host.Of these cytokines, the expression of interleukin-6 decreased remarkably after Duolac-Gold treatment.Taken together, these results suggest that Duolac-Gold treatment is effective in IBD healing by regulating IL-6.

View Article: PubMed Central - PubMed

Affiliation: R&D Center, Cellbiotech Co. Ltd., Gimpo, Korea.

ABSTRACT
Probiotics are live microorganisms that confer a health benefit on the host. Duolac-Gold is a mixture of six probiotic bacteria containing three species of Bifidobacteria, two species of Lactobacillus, and Streptococcus thermophilus [corrected]. The aim of this study was to assess the anti-inflammatory effects of Duolac-Gold in an inflammatory bowel disease (IBD) mouse model. IBD was induced by administering 1.5% dextran sulfate sodium (DSS) for 10 days. After induction of DSS-induced colitis, Duolac-Gold was orally administered at three different concentrations. Interestingly, Duolac-Gold treatment accelerated IBD healing, and anti-inflammatory activity was assessed by weight loss, length of the colon, and a microscopic damage score by histology. The expression of inflammatory related cytokines was measured in colon tissues and serum. Of these cytokines, the expression of interleukin-6 decreased remarkably after Duolac-Gold treatment. Taken together, these results suggest that Duolac-Gold treatment is effective in IBD healing by regulating IL-6.

No MeSH data available.


Related in: MedlinePlus