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Direct conversion of pretreated straw cellulose into citric acid by co-cultures of Yarrowia lipolytica SWJ-1b and immobilized Trichoderma reesei mycelium.

Liu X, Lv J, Zhang T, Deng Y - Appl. Biochem. Biotechnol. (2014)

Bottom Line: The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain.When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h.Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h.

View Article: PubMed Central - PubMed

ABSTRACT
The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h.

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Time course of total sugar change, reducing sugar change, cellulose activity, CA yield, and isocitric acid yield in the PSM. Data are given as mean ± SD, n = 3
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Fig3: Time course of total sugar change, reducing sugar change, cellulose activity, CA yield, and isocitric acid yield in the PSM. Data are given as mean ± SD, n = 3

Mentions: After the immobilized mycelia of T. reesei were co-cultivated with the free cells of Y. lipolytica SWJ-1b in the PSM containing 40.0 g/l pretreated straw, the concentration of CA and ICA in the fermentation broth was determined as described in “Materials and Methods”. The result in Fig. 3 shows that the CA and ICA yields of Y. lipolytica SWJ-1b were 10.7 g/l (reached to the maximum at 192 h) and 3.9 g/l, respectively, and the cellulase activity reached to 2.3 U/ml at 168 h and persisted until the end of the cultivation (240 h). The results in Fig. 3 also indicate that 82.5 % of pretreated straw in the medium was utilized by the co-cultures. When only Y. lipolytica SWJ-1b was inoculated into the medium containing 40.0 g/l pretreated straw, there was no growth (data not shown).Fig. 3


Direct conversion of pretreated straw cellulose into citric acid by co-cultures of Yarrowia lipolytica SWJ-1b and immobilized Trichoderma reesei mycelium.

Liu X, Lv J, Zhang T, Deng Y - Appl. Biochem. Biotechnol. (2014)

Time course of total sugar change, reducing sugar change, cellulose activity, CA yield, and isocitric acid yield in the PSM. Data are given as mean ± SD, n = 3
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4007025&req=5

Fig3: Time course of total sugar change, reducing sugar change, cellulose activity, CA yield, and isocitric acid yield in the PSM. Data are given as mean ± SD, n = 3
Mentions: After the immobilized mycelia of T. reesei were co-cultivated with the free cells of Y. lipolytica SWJ-1b in the PSM containing 40.0 g/l pretreated straw, the concentration of CA and ICA in the fermentation broth was determined as described in “Materials and Methods”. The result in Fig. 3 shows that the CA and ICA yields of Y. lipolytica SWJ-1b were 10.7 g/l (reached to the maximum at 192 h) and 3.9 g/l, respectively, and the cellulase activity reached to 2.3 U/ml at 168 h and persisted until the end of the cultivation (240 h). The results in Fig. 3 also indicate that 82.5 % of pretreated straw in the medium was utilized by the co-cultures. When only Y. lipolytica SWJ-1b was inoculated into the medium containing 40.0 g/l pretreated straw, there was no growth (data not shown).Fig. 3

Bottom Line: The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain.When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h.Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h.

View Article: PubMed Central - PubMed

ABSTRACT
The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h.

Show MeSH
Related in: MedlinePlus