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Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat.

Li W, Wu Y, Zhu C, Wang Z, Gao R, Wu Q - Indian J Pharmacol (2014 Mar-Apr)

Bottom Line: Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively.The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.

ABSTRACT

Aim: Some gradient of Huisheng oral solution (HOS) has been reported to have anti-fibrosis activity. This study was designed to investigate whether HOS could inhibit liver fibrosis and to elucidate its molecular mechanism of action.

Materials and methods: Hepatic fibrosis model in rat was induced by subcutaneous injection of CCl4. Rats in the treatment group were administrated with HOS intragastrically. Hematoxylin and eosin (H and E) staining and Masson's trichrome staining were used to examine the changes in liver pathology. Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively. The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.

Results: Our data demonstrated that HOS alleviated CCl4-induced collagen deposition in liver tissue, improved liver condition and liver function in rats. HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

Conclusions: This study revealed that HOS attenuates the development of liver fibrosis through suppressing the TGF-β1 pathway. It provides us a new approach to treatment of liver fibrosis.

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Related in: MedlinePlus

Huisheng oral solution decreased the mRNA (A1, A2) and protein (B1, B2) expressions of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue. The mRNA and protein levels of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue were showed by qPCR (A1) and western blot (B1), respectively. Their relative expressions were respectively normalized to those of GADPH (A2) and β-actin (B2), respectively. *P <0.05 compared with the control group; *P <0.05 compared with the model group.
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Figure 3: Huisheng oral solution decreased the mRNA (A1, A2) and protein (B1, B2) expressions of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue. The mRNA and protein levels of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue were showed by qPCR (A1) and western blot (B1), respectively. Their relative expressions were respectively normalized to those of GADPH (A2) and β-actin (B2), respectively. *P <0.05 compared with the control group; *P <0.05 compared with the model group.

Mentions: As shown in Figure 3, the mRNA and protein expressions of Smad3, TGFβ1, α-SMA and TIMP-1 in liver tissue were significantly increased in the model group compared with the control group (P < 0.05, respectively). However, Huisheng oral solution apparently decreased all the above parameters compared with the model group (P < 0.05, respectively). Therefore, Huisheng oral solution could significantly reduce the expression of α-SMA which may be associated with decreased TGFβ1 activation.


Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat.

Li W, Wu Y, Zhu C, Wang Z, Gao R, Wu Q - Indian J Pharmacol (2014 Mar-Apr)

Huisheng oral solution decreased the mRNA (A1, A2) and protein (B1, B2) expressions of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue. The mRNA and protein levels of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue were showed by qPCR (A1) and western blot (B1), respectively. Their relative expressions were respectively normalized to those of GADPH (A2) and β-actin (B2), respectively. *P <0.05 compared with the control group; *P <0.05 compared with the model group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987194&req=5

Figure 3: Huisheng oral solution decreased the mRNA (A1, A2) and protein (B1, B2) expressions of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue. The mRNA and protein levels of Smad3, TGF-β1, α-SMA and TIMP-1 in liver tissue were showed by qPCR (A1) and western blot (B1), respectively. Their relative expressions were respectively normalized to those of GADPH (A2) and β-actin (B2), respectively. *P <0.05 compared with the control group; *P <0.05 compared with the model group.
Mentions: As shown in Figure 3, the mRNA and protein expressions of Smad3, TGFβ1, α-SMA and TIMP-1 in liver tissue were significantly increased in the model group compared with the control group (P < 0.05, respectively). However, Huisheng oral solution apparently decreased all the above parameters compared with the model group (P < 0.05, respectively). Therefore, Huisheng oral solution could significantly reduce the expression of α-SMA which may be associated with decreased TGFβ1 activation.

Bottom Line: Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively.The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.

ABSTRACT

Aim: Some gradient of Huisheng oral solution (HOS) has been reported to have anti-fibrosis activity. This study was designed to investigate whether HOS could inhibit liver fibrosis and to elucidate its molecular mechanism of action.

Materials and methods: Hepatic fibrosis model in rat was induced by subcutaneous injection of CCl4. Rats in the treatment group were administrated with HOS intragastrically. Hematoxylin and eosin (H and E) staining and Masson's trichrome staining were used to examine the changes in liver pathology. Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively. The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.

Results: Our data demonstrated that HOS alleviated CCl4-induced collagen deposition in liver tissue, improved liver condition and liver function in rats. HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

Conclusions: This study revealed that HOS attenuates the development of liver fibrosis through suppressing the TGF-β1 pathway. It provides us a new approach to treatment of liver fibrosis.

Show MeSH
Related in: MedlinePlus