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Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat.

Li W, Wu Y, Zhu C, Wang Z, Gao R, Wu Q - Indian J Pharmacol (2014 Mar-Apr)

Bottom Line: Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively.The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.

ABSTRACT

Aim: Some gradient of Huisheng oral solution (HOS) has been reported to have anti-fibrosis activity. This study was designed to investigate whether HOS could inhibit liver fibrosis and to elucidate its molecular mechanism of action.

Materials and methods: Hepatic fibrosis model in rat was induced by subcutaneous injection of CCl4. Rats in the treatment group were administrated with HOS intragastrically. Hematoxylin and eosin (H and E) staining and Masson's trichrome staining were used to examine the changes in liver pathology. Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively. The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.

Results: Our data demonstrated that HOS alleviated CCl4-induced collagen deposition in liver tissue, improved liver condition and liver function in rats. HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

Conclusions: This study revealed that HOS attenuates the development of liver fibrosis through suppressing the TGF-β1 pathway. It provides us a new approach to treatment of liver fibrosis.

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Related in: MedlinePlus

Expressions of Smad3 (A1-3), TGF-β1 (B1-3), α-SMA (C1-3), TIMP-1 (D1-3) in liver tissue (×200). Lane 1, rats in control group; Lane 2: rats in the treatment group (Huisheng oral solution); Lane 3: rats in model group (normal saline+CCl4). Considerable expression of Smad3 protein was observed among the periportal fibrotic areas, central vein, and fibrous septa in model group (A3) compared with normal group (A1). Huisheng oral solution treatment reduced the expression of Smad3 compared with the model group (A2). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3
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Figure 2: Expressions of Smad3 (A1-3), TGF-β1 (B1-3), α-SMA (C1-3), TIMP-1 (D1-3) in liver tissue (×200). Lane 1, rats in control group; Lane 2: rats in the treatment group (Huisheng oral solution); Lane 3: rats in model group (normal saline+CCl4). Considerable expression of Smad3 protein was observed among the periportal fibrotic areas, central vein, and fibrous septa in model group (A3) compared with normal group (A1). Huisheng oral solution treatment reduced the expression of Smad3 compared with the model group (A2). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3

Mentions: Immunohistochemical analysis was used to detect the expression and distribution of Smad3, TGFβ1, α-SMA and TIMP-1 in liver tissue. As revealed in Figure 2, there were few Smad3-positive regions in the control group (A1, PI = 0.0067 ± 0.006). In contrast, the expression of Smad3 was significantly increased in the model group, and Smad3-positive regions can be seen around the periportal fibrotic band areas, central vein and fibrous septa (A3, P1 = 0.0563 ± 0.007, P < 0.05), whereas they were sharply down-regulated in the Huisheng oral solution-treated group (A2, PI = 0.0325 ± 0.004, P < 0.05). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3. (TGF-β1, 0.0055 ± 0.007 vs 0.0545 ± 0.006 vs 0.0344 ± 0.005; α-SMA, 0.0067 ± 0.008 vs 0.0643 ± 0.009 vs 0.0432 ± 0.010; TIMP-1, 0.0023 ± 0.009 vs 0.0531 ± 0.011 vs 0.0441 ± 0.010; P < 0.05, respectively).


Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat.

Li W, Wu Y, Zhu C, Wang Z, Gao R, Wu Q - Indian J Pharmacol (2014 Mar-Apr)

Expressions of Smad3 (A1-3), TGF-β1 (B1-3), α-SMA (C1-3), TIMP-1 (D1-3) in liver tissue (×200). Lane 1, rats in control group; Lane 2: rats in the treatment group (Huisheng oral solution); Lane 3: rats in model group (normal saline+CCl4). Considerable expression of Smad3 protein was observed among the periportal fibrotic areas, central vein, and fibrous septa in model group (A3) compared with normal group (A1). Huisheng oral solution treatment reduced the expression of Smad3 compared with the model group (A2). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987194&req=5

Figure 2: Expressions of Smad3 (A1-3), TGF-β1 (B1-3), α-SMA (C1-3), TIMP-1 (D1-3) in liver tissue (×200). Lane 1, rats in control group; Lane 2: rats in the treatment group (Huisheng oral solution); Lane 3: rats in model group (normal saline+CCl4). Considerable expression of Smad3 protein was observed among the periportal fibrotic areas, central vein, and fibrous septa in model group (A3) compared with normal group (A1). Huisheng oral solution treatment reduced the expression of Smad3 compared with the model group (A2). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3
Mentions: Immunohistochemical analysis was used to detect the expression and distribution of Smad3, TGFβ1, α-SMA and TIMP-1 in liver tissue. As revealed in Figure 2, there were few Smad3-positive regions in the control group (A1, PI = 0.0067 ± 0.006). In contrast, the expression of Smad3 was significantly increased in the model group, and Smad3-positive regions can be seen around the periportal fibrotic band areas, central vein and fibrous septa (A3, P1 = 0.0563 ± 0.007, P < 0.05), whereas they were sharply down-regulated in the Huisheng oral solution-treated group (A2, PI = 0.0325 ± 0.004, P < 0.05). The expression of TGF-β1, α-SMA and TIMP-1 was consistent with Smad3. (TGF-β1, 0.0055 ± 0.007 vs 0.0545 ± 0.006 vs 0.0344 ± 0.005; α-SMA, 0.0067 ± 0.008 vs 0.0643 ± 0.009 vs 0.0432 ± 0.010; TIMP-1, 0.0023 ± 0.009 vs 0.0531 ± 0.011 vs 0.0441 ± 0.010; P < 0.05, respectively).

Bottom Line: Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively.The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.

ABSTRACT

Aim: Some gradient of Huisheng oral solution (HOS) has been reported to have anti-fibrosis activity. This study was designed to investigate whether HOS could inhibit liver fibrosis and to elucidate its molecular mechanism of action.

Materials and methods: Hepatic fibrosis model in rat was induced by subcutaneous injection of CCl4. Rats in the treatment group were administrated with HOS intragastrically. Hematoxylin and eosin (H and E) staining and Masson's trichrome staining were used to examine the changes in liver pathology. Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively. The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.

Results: Our data demonstrated that HOS alleviated CCl4-induced collagen deposition in liver tissue, improved liver condition and liver function in rats. HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.

Conclusions: This study revealed that HOS attenuates the development of liver fibrosis through suppressing the TGF-β1 pathway. It provides us a new approach to treatment of liver fibrosis.

Show MeSH
Related in: MedlinePlus