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Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism.

Kosaraju J, Chinni S, Roy PD, Kannan E, Antony AS, Kumar MN - Indian J Pharmacol (2014 Mar-Apr)

Bottom Line: Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 ± 0.18) and 400 mg/kg (1.11 ± 0.15) when compared with negative control group.Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamadalam, Tamil Nadu, India.

ABSTRACT

Objective: The present study investigates the neuroprotective activity of ethanol extract of Tinospora cordifolia aerial parts against 6-hydroxy dopamine (6-OHDA) lesion rat model of Parkinson's disease (PD).

Materials and methods: T. cordifolia ethanol extract (TCEE) was standardized with high performance thin layer chromatography using berberine. Experimental PD was induced by intracerebral injection of 6-OHDA (8 μg). Animals were divided into five groups: sham operated, negative control, positive control (levodopa 6 mg/kg) and two experimental groups (n = 6/group). Experimental groups received 200 and 400 mg/kg of TCEE once daily for 30 days by oral gavage. Biochemical parameters including dopamine level, oxidative stress, complex I activity and brain iron asymmetry ratio and locomotor activity including skeletal muscle co-ordination and degree of catatonia were assessed.

Results: TCEE exhibited significant neuroprotection by increasing the dopamine levels (1.96 ± 0.20 and 2.45 ± 0.40 ng/mg of protein) and complex I activity (77.14 ± 0.89 and 78.50 ± 0.96 nmol/min/mg of protein) at 200 and 400 mg/kg respectively when compared with negative control group. Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 ± 0.18) and 400 mg/kg (1.11 ± 0.15) when compared with negative control group. Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.

Conclusion: Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.

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Related in: MedlinePlus

Effect of Tinospora cordifolia ethanol extract treatment for a period of 30 days on brain iron asymmetry ratio. Graph represented as mean ± standard deviation (n = 6). ###P < 0.001 when compared with sham operated; ***P < 0.001, **P < 0.01 when compared with 6-OHDA. L-DOPA: Levodopa; 6-OHDA: 6-hydroxy dopamine; TCEE: T. cordifolia ethanol extract
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Figure 4: Effect of Tinospora cordifolia ethanol extract treatment for a period of 30 days on brain iron asymmetry ratio. Graph represented as mean ± standard deviation (n = 6). ###P < 0.001 when compared with sham operated; ***P < 0.001, **P < 0.01 when compared with 6-OHDA. L-DOPA: Levodopa; 6-OHDA: 6-hydroxy dopamine; TCEE: T. cordifolia ethanol extract

Mentions: SN iron localization studies clearly indicate that the iron deposition or iron asymmetry ratio for 6-OHDA was significantly (P < 0.001) increased when compared with sham control group. L-DOPA treated groups showed significantly (P < 0.01) increase in iron asymmetry ratio when compared with negative control group. TCEE treated groups dose-dependently and significantly reduced the iron deposition after 30 days treatment when compared with negative control group [Figures 4 and 5].


Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism.

Kosaraju J, Chinni S, Roy PD, Kannan E, Antony AS, Kumar MN - Indian J Pharmacol (2014 Mar-Apr)

Effect of Tinospora cordifolia ethanol extract treatment for a period of 30 days on brain iron asymmetry ratio. Graph represented as mean ± standard deviation (n = 6). ###P < 0.001 when compared with sham operated; ***P < 0.001, **P < 0.01 when compared with 6-OHDA. L-DOPA: Levodopa; 6-OHDA: 6-hydroxy dopamine; TCEE: T. cordifolia ethanol extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987186&req=5

Figure 4: Effect of Tinospora cordifolia ethanol extract treatment for a period of 30 days on brain iron asymmetry ratio. Graph represented as mean ± standard deviation (n = 6). ###P < 0.001 when compared with sham operated; ***P < 0.001, **P < 0.01 when compared with 6-OHDA. L-DOPA: Levodopa; 6-OHDA: 6-hydroxy dopamine; TCEE: T. cordifolia ethanol extract
Mentions: SN iron localization studies clearly indicate that the iron deposition or iron asymmetry ratio for 6-OHDA was significantly (P < 0.001) increased when compared with sham control group. L-DOPA treated groups showed significantly (P < 0.01) increase in iron asymmetry ratio when compared with negative control group. TCEE treated groups dose-dependently and significantly reduced the iron deposition after 30 days treatment when compared with negative control group [Figures 4 and 5].

Bottom Line: Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 ± 0.18) and 400 mg/kg (1.11 ± 0.15) when compared with negative control group.Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamadalam, Tamil Nadu, India.

ABSTRACT

Objective: The present study investigates the neuroprotective activity of ethanol extract of Tinospora cordifolia aerial parts against 6-hydroxy dopamine (6-OHDA) lesion rat model of Parkinson's disease (PD).

Materials and methods: T. cordifolia ethanol extract (TCEE) was standardized with high performance thin layer chromatography using berberine. Experimental PD was induced by intracerebral injection of 6-OHDA (8 μg). Animals were divided into five groups: sham operated, negative control, positive control (levodopa 6 mg/kg) and two experimental groups (n = 6/group). Experimental groups received 200 and 400 mg/kg of TCEE once daily for 30 days by oral gavage. Biochemical parameters including dopamine level, oxidative stress, complex I activity and brain iron asymmetry ratio and locomotor activity including skeletal muscle co-ordination and degree of catatonia were assessed.

Results: TCEE exhibited significant neuroprotection by increasing the dopamine levels (1.96 ± 0.20 and 2.45 ± 0.40 ng/mg of protein) and complex I activity (77.14 ± 0.89 and 78.50 ± 0.96 nmol/min/mg of protein) at 200 and 400 mg/kg respectively when compared with negative control group. Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 ± 0.18) and 400 mg/kg (1.11 ± 0.15) when compared with negative control group. Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.

Conclusion: Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.

Show MeSH
Related in: MedlinePlus