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Prognostic significance of HLA EMR8-5 immunohistochemically analyzed expression in osteosarcoma.

Nada OH, Ahmed NS, Abou Gabal HH - Diagn Pathol (2014)

Bottom Line: The researchers investigated HLA class I expression in primary osteosarcoma by immunohistochemistry using EMR8-5 mAbs.Furthermore, researchers evaluated the correlation between HLA class I expression and the clinicopathological status and outcome in formalin fixed paraffin embedded tissues from thirty six (36) patients with osteosarcoma.Down-regulation of class I antigen expression is associated with features of aggressive disease and a poorer prognosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of pathology, faculty of Medicine, Ain Shams university, Cairo, Egypt. dr_olanada@yahoo.com.

ABSTRACT

Background: Defects in Human Leukocyte Antigen (HLA) class I antigen expression and/or function in tumor cells have been extensively investigated, because of their potential role in the escape of tumor cells from T cell recognition and destruction. The researchers evaluated HLA class I expression in tumor tissue as a prognostic factor in osteosarcoma patients and as a predictor of their survival. This retrospective cohort study was conducted at the pathology laboratory of Ain Shams University Hospital, and Ain Shams University Specialized Hospital during the period between January 2009 and January 2012.

Methods: The researchers investigated HLA class I expression in primary osteosarcoma by immunohistochemistry using EMR8-5 mAbs. Furthermore, researchers evaluated the correlation between HLA class I expression and the clinicopathological status and outcome in formalin fixed paraffin embedded tissues from thirty six (36) patients with osteosarcoma.

Results: A high expression of HLA class I was detected in 18 (50) % of tumor samples examined; while tumors with low or negative expression represented 9 (25%) cases each. Data indicate that the overall survival rate of patients with tumors highly expressing HLA class I was significantly higher than those with low or negative expression.

Conclusion: Down-regulation of class I antigen expression is associated with features of aggressive disease and a poorer prognosis. Therefore, it is imperative to identify HLA as a prognostic factor at the time of diagnosis to detect chemotherapy-resistant tumors and to generate a modified treatment regimen.

Virtual slides: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1159334857109547.

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Kaplan-Meier overall survival curve in relation to immunohistochemistry scores for HLA class I.
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Figure 1: Kaplan-Meier overall survival curve in relation to immunohistochemistry scores for HLA class I.

Mentions: Through the use of univariate analysis of the overall and the disease free survival in relation to the HLA class I expression (Table 2), Log Rank tests performed on Kaplan – Meier survival curves disclosed a highly significant differences in overall and disease free survival (P = .0001 for both) (Figures 1 and 2). Patients who had high expression of HLA class I in their initial biopsies showed a significant better overall survival compared to those with low expression of HLA class I, or those with negative expression. As regards the disease free survival, patients with osteosarcoma highly expressing HLA class I had a significantly better disease-free survival compared to those with negative expression. Similarly, osteosarcomas with low expression of HLA class I showed a significantly longer disease-free survival when compared to those with negative expression. When excluding patients presented with metastatic disease, there were still a significant differences between HLA class I expression, on the one hand, and overall (P = .018) and disease free survival (P = .001), on the other.


Prognostic significance of HLA EMR8-5 immunohistochemically analyzed expression in osteosarcoma.

Nada OH, Ahmed NS, Abou Gabal HH - Diagn Pathol (2014)

Kaplan-Meier overall survival curve in relation to immunohistochemistry scores for HLA class I.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3987053&req=5

Figure 1: Kaplan-Meier overall survival curve in relation to immunohistochemistry scores for HLA class I.
Mentions: Through the use of univariate analysis of the overall and the disease free survival in relation to the HLA class I expression (Table 2), Log Rank tests performed on Kaplan – Meier survival curves disclosed a highly significant differences in overall and disease free survival (P = .0001 for both) (Figures 1 and 2). Patients who had high expression of HLA class I in their initial biopsies showed a significant better overall survival compared to those with low expression of HLA class I, or those with negative expression. As regards the disease free survival, patients with osteosarcoma highly expressing HLA class I had a significantly better disease-free survival compared to those with negative expression. Similarly, osteosarcomas with low expression of HLA class I showed a significantly longer disease-free survival when compared to those with negative expression. When excluding patients presented with metastatic disease, there were still a significant differences between HLA class I expression, on the one hand, and overall (P = .018) and disease free survival (P = .001), on the other.

Bottom Line: The researchers investigated HLA class I expression in primary osteosarcoma by immunohistochemistry using EMR8-5 mAbs.Furthermore, researchers evaluated the correlation between HLA class I expression and the clinicopathological status and outcome in formalin fixed paraffin embedded tissues from thirty six (36) patients with osteosarcoma.Down-regulation of class I antigen expression is associated with features of aggressive disease and a poorer prognosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of pathology, faculty of Medicine, Ain Shams university, Cairo, Egypt. dr_olanada@yahoo.com.

ABSTRACT

Background: Defects in Human Leukocyte Antigen (HLA) class I antigen expression and/or function in tumor cells have been extensively investigated, because of their potential role in the escape of tumor cells from T cell recognition and destruction. The researchers evaluated HLA class I expression in tumor tissue as a prognostic factor in osteosarcoma patients and as a predictor of their survival. This retrospective cohort study was conducted at the pathology laboratory of Ain Shams University Hospital, and Ain Shams University Specialized Hospital during the period between January 2009 and January 2012.

Methods: The researchers investigated HLA class I expression in primary osteosarcoma by immunohistochemistry using EMR8-5 mAbs. Furthermore, researchers evaluated the correlation between HLA class I expression and the clinicopathological status and outcome in formalin fixed paraffin embedded tissues from thirty six (36) patients with osteosarcoma.

Results: A high expression of HLA class I was detected in 18 (50) % of tumor samples examined; while tumors with low or negative expression represented 9 (25%) cases each. Data indicate that the overall survival rate of patients with tumors highly expressing HLA class I was significantly higher than those with low or negative expression.

Conclusion: Down-regulation of class I antigen expression is associated with features of aggressive disease and a poorer prognosis. Therefore, it is imperative to identify HLA as a prognostic factor at the time of diagnosis to detect chemotherapy-resistant tumors and to generate a modified treatment regimen.

Virtual slides: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1159334857109547.

Show MeSH
Related in: MedlinePlus