Limits...
Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.

Chudobova D, Dostalova S, Blazkova I, Michalek P, Ruttkay-Nedecky B, Sklenar M, Nejdl L, Kudr J, Gumulec J, Tmejova K, Konecna M, Vaculovicova M, Hynek D, Masarik M, Kynicky J, Kizek R, Adam V - Int J Environ Res Public Health (2014)

Bottom Line: There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections.An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves.A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dagmar.chudobova@centrum.cz.

ABSTRACT
There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Show MeSH

Related in: MedlinePlus

Interaction of bacterial DNA fragment with different concentrations of heavy metal ions ( 0,  60,  240 and  950 μM): (A) Interaction of 16S gene with cadmium ions, (a) decrease of absorbance of 16S gene with cadmium ions in 260 nm. (B) Interaction of 16S gene with lead ions, (b) decrease of absorbance of 16S gene with lead ions in 260 nm. (C) Interaction of zntR gene with cadmium ions, (c) decrease of absorbance of zntR gene with cadmium ions in 260 nm. (D) Interaction of zntR gene with lead ions, (d) decrease of absorbance of zntR gene with lead ions in 260 nm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3987032&req=5

ijerph-11-03233-f004: Interaction of bacterial DNA fragment with different concentrations of heavy metal ions ( 0, 60, 240 and 950 μM): (A) Interaction of 16S gene with cadmium ions, (a) decrease of absorbance of 16S gene with cadmium ions in 260 nm. (B) Interaction of 16S gene with lead ions, (b) decrease of absorbance of 16S gene with lead ions in 260 nm. (C) Interaction of zntR gene with cadmium ions, (c) decrease of absorbance of zntR gene with cadmium ions in 260 nm. (D) Interaction of zntR gene with lead ions, (d) decrease of absorbance of zntR gene with lead ions in 260 nm.

Mentions: To confirm the simplified penetration of heavy metal ions into the cellular DNA, the interaction of cadmium and lead ions with a bacterial DNA fragment zntR and 16S was studied. This phenomenon was studied by spectrometric methods, particularly by absorbance measurement (Figure 4). The presence of heavy metal ions in DNA was manifested by a significant decrease of records for each concentration in comparison with a control sample. These results confirm the easy interaction of cadmium and lead ions with bacterial DNA.


Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.

Chudobova D, Dostalova S, Blazkova I, Michalek P, Ruttkay-Nedecky B, Sklenar M, Nejdl L, Kudr J, Gumulec J, Tmejova K, Konecna M, Vaculovicova M, Hynek D, Masarik M, Kynicky J, Kizek R, Adam V - Int J Environ Res Public Health (2014)

Interaction of bacterial DNA fragment with different concentrations of heavy metal ions ( 0,  60,  240 and  950 μM): (A) Interaction of 16S gene with cadmium ions, (a) decrease of absorbance of 16S gene with cadmium ions in 260 nm. (B) Interaction of 16S gene with lead ions, (b) decrease of absorbance of 16S gene with lead ions in 260 nm. (C) Interaction of zntR gene with cadmium ions, (c) decrease of absorbance of zntR gene with cadmium ions in 260 nm. (D) Interaction of zntR gene with lead ions, (d) decrease of absorbance of zntR gene with lead ions in 260 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987032&req=5

ijerph-11-03233-f004: Interaction of bacterial DNA fragment with different concentrations of heavy metal ions ( 0, 60, 240 and 950 μM): (A) Interaction of 16S gene with cadmium ions, (a) decrease of absorbance of 16S gene with cadmium ions in 260 nm. (B) Interaction of 16S gene with lead ions, (b) decrease of absorbance of 16S gene with lead ions in 260 nm. (C) Interaction of zntR gene with cadmium ions, (c) decrease of absorbance of zntR gene with cadmium ions in 260 nm. (D) Interaction of zntR gene with lead ions, (d) decrease of absorbance of zntR gene with lead ions in 260 nm.
Mentions: To confirm the simplified penetration of heavy metal ions into the cellular DNA, the interaction of cadmium and lead ions with a bacterial DNA fragment zntR and 16S was studied. This phenomenon was studied by spectrometric methods, particularly by absorbance measurement (Figure 4). The presence of heavy metal ions in DNA was manifested by a significant decrease of records for each concentration in comparison with a control sample. These results confirm the easy interaction of cadmium and lead ions with bacterial DNA.

Bottom Line: There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections.An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves.A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dagmar.chudobova@centrum.cz.

ABSTRACT
There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Show MeSH
Related in: MedlinePlus