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Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.

Chudobova D, Dostalova S, Blazkova I, Michalek P, Ruttkay-Nedecky B, Sklenar M, Nejdl L, Kudr J, Gumulec J, Tmejova K, Konecna M, Vaculovicova M, Hynek D, Masarik M, Kynicky J, Kizek R, Adam V - Int J Environ Res Public Health (2014)

Bottom Line: There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections.An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves.A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dagmar.chudobova@centrum.cz.

ABSTRACT
There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

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Images of S. aureus cells using microscopy in ambient light: (A) Micrographs of cells with resistance to metal ions (a) non-resistant strain of S. aureus; (b) RCd; (c) RPb. Parameters were as follows: Device: Microscopy; Volume: 5 μL; Zoom: 1600×; Ambient light; Exp.t.: 32.05 ms; ISO 200; Resolution: 4800 × 3600. (B) Micrographs of cells with resistance to metal ions after the application of antibiotics (50 µM): (a) penicillin; (b) streptomycin; (c) ampicillin; (d) tetracycline on non-resistant strain of S. aureus; (e) penicillin (f) streptomycin (g) ampicillin (h) tetracycline on RCd; or (i) penicillin (j) streptomycin (k) ampicillin (l) tetracycline on RPb.
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ijerph-11-03233-f001: Images of S. aureus cells using microscopy in ambient light: (A) Micrographs of cells with resistance to metal ions (a) non-resistant strain of S. aureus; (b) RCd; (c) RPb. Parameters were as follows: Device: Microscopy; Volume: 5 μL; Zoom: 1600×; Ambient light; Exp.t.: 32.05 ms; ISO 200; Resolution: 4800 × 3600. (B) Micrographs of cells with resistance to metal ions after the application of antibiotics (50 µM): (a) penicillin; (b) streptomycin; (c) ampicillin; (d) tetracycline on non-resistant strain of S. aureus; (e) penicillin (f) streptomycin (g) ampicillin (h) tetracycline on RCd; or (i) penicillin (j) streptomycin (k) ampicillin (l) tetracycline on RPb.

Mentions: Significant morphological changes were observed in the cells in terms of cell shapes. The presence of so called cross walls (septal midline) was observed (Figure 1). These inner transverse walls were formed due to the development of the resistance. Similar phenomenon is commonly observed in case of MRSA strains [29,30,31]. The above mentioned morphological changes were observed in both, metal resistant strains and control strain, after application of antibiotics. The detected phenomenon was the evidence of developing multi-resistance due to the fact that characteristic morphological hallmarks were present after addition of metal ions as well as antibiotics. Cell morphology was not influenced by the antibiotic administration due to the fact that the morphology was changed already by application of metal ions.


Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.

Chudobova D, Dostalova S, Blazkova I, Michalek P, Ruttkay-Nedecky B, Sklenar M, Nejdl L, Kudr J, Gumulec J, Tmejova K, Konecna M, Vaculovicova M, Hynek D, Masarik M, Kynicky J, Kizek R, Adam V - Int J Environ Res Public Health (2014)

Images of S. aureus cells using microscopy in ambient light: (A) Micrographs of cells with resistance to metal ions (a) non-resistant strain of S. aureus; (b) RCd; (c) RPb. Parameters were as follows: Device: Microscopy; Volume: 5 μL; Zoom: 1600×; Ambient light; Exp.t.: 32.05 ms; ISO 200; Resolution: 4800 × 3600. (B) Micrographs of cells with resistance to metal ions after the application of antibiotics (50 µM): (a) penicillin; (b) streptomycin; (c) ampicillin; (d) tetracycline on non-resistant strain of S. aureus; (e) penicillin (f) streptomycin (g) ampicillin (h) tetracycline on RCd; or (i) penicillin (j) streptomycin (k) ampicillin (l) tetracycline on RPb.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987032&req=5

ijerph-11-03233-f001: Images of S. aureus cells using microscopy in ambient light: (A) Micrographs of cells with resistance to metal ions (a) non-resistant strain of S. aureus; (b) RCd; (c) RPb. Parameters were as follows: Device: Microscopy; Volume: 5 μL; Zoom: 1600×; Ambient light; Exp.t.: 32.05 ms; ISO 200; Resolution: 4800 × 3600. (B) Micrographs of cells with resistance to metal ions after the application of antibiotics (50 µM): (a) penicillin; (b) streptomycin; (c) ampicillin; (d) tetracycline on non-resistant strain of S. aureus; (e) penicillin (f) streptomycin (g) ampicillin (h) tetracycline on RCd; or (i) penicillin (j) streptomycin (k) ampicillin (l) tetracycline on RPb.
Mentions: Significant morphological changes were observed in the cells in terms of cell shapes. The presence of so called cross walls (septal midline) was observed (Figure 1). These inner transverse walls were formed due to the development of the resistance. Similar phenomenon is commonly observed in case of MRSA strains [29,30,31]. The above mentioned morphological changes were observed in both, metal resistant strains and control strain, after application of antibiotics. The detected phenomenon was the evidence of developing multi-resistance due to the fact that characteristic morphological hallmarks were present after addition of metal ions as well as antibiotics. Cell morphology was not influenced by the antibiotic administration due to the fact that the morphology was changed already by application of metal ions.

Bottom Line: There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections.An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves.A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dagmar.chudobova@centrum.cz.

ABSTRACT
There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Show MeSH
Related in: MedlinePlus