Limits...
Toxicity and estrogenic endocrine disrupting activity of phthalates and their mixtures.

Chen X, Xu S, Tan T, Lee ST, Cheng SH, Lee FW, Xu SJ, Ho KC - Int J Environ Res Public Health (2014)

Bottom Line: Phthalates, widely used in flexible plastics and consumer products, have become ubiquitous contaminants worldwide.The other four phthalates did not cause more than 50% exposed embryo mortality even at their highest soluble concentrations.Using an estrogen-responsive ChgH-EGFP transgenic medaka (Oryzias melastigma) eleutheroembryos based 24 h test, BBP demonstrated estrogenic activity, DBP, DEHP, DINP and the mixture of the six phthalates exhibited enhanced-estrogenic activity and DIDP and DNOP showed no enhanced- or anti-estrogenic activity.

View Article: PubMed Central - PubMed

Affiliation: Vitargent (International) Biotechnology Limited, Unit 516, 5/F. Biotech Centre 2, No. 11 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong. xueping.chen@hotmail.com.

ABSTRACT
Phthalates, widely used in flexible plastics and consumer products, have become ubiquitous contaminants worldwide. This study evaluated the acute toxicity and estrogenic endocrine disrupting activity of butyl benzyl phthalate (BBP), di(n-butyl) phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), di-n-octyl phthalate (DNOP) and their mixtures. Using a 72 h zebrafish embryo toxicity test, the LC50 values of BBP, DBP and a mixture of the six phthalates were found to be 0.72, 0.63 and 0.50 ppm, respectively. The other four phthalates did not cause more than 50% exposed embryo mortality even at their highest soluble concentrations. The typical toxicity symptoms caused by phthalates were death, tail curvature, necrosis, cardio edema and no touch response. Using an estrogen-responsive ChgH-EGFP transgenic medaka (Oryzias melastigma) eleutheroembryos based 24 h test, BBP demonstrated estrogenic activity, DBP, DEHP, DINP and the mixture of the six phthalates exhibited enhanced-estrogenic activity and DIDP and DNOP showed no enhanced- or anti-estrogenic activity. These findings highlighted the developmental toxicity of BBP and DBP, and the estrogenic endocrine disrupting activity of BBP, DBP, DEHP and DINP on intact organisms, indicating that the widespread use of these phthalates may cause potential health risks to human beings.

Show MeSH

Related in: MedlinePlus

Enhanced-estrogenic activity of phthalates. DBP (1.00 ppm), DEHP (1.50 ppm) and DINP (1.50 ppm) significantly increased, and DIDP (1.5 ppm) and DNOP (1.5 ppm) did not statistically increase or decrease, the GFP signal intensity (RFU) of the livers of transgenic medaka (O. melastigma) eleutheroembryos induced by E2 (2.5 ppb).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3987027&req=5

ijerph-11-03156-f005: Enhanced-estrogenic activity of phthalates. DBP (1.00 ppm), DEHP (1.50 ppm) and DINP (1.50 ppm) significantly increased, and DIDP (1.5 ppm) and DNOP (1.5 ppm) did not statistically increase or decrease, the GFP signal intensity (RFU) of the livers of transgenic medaka (O. melastigma) eleutheroembryos induced by E2 (2.5 ppb).

Mentions: Results showed that 1.00 ppm DBP, 1.50 ppm DEHP or DINP significantly increased the green fluorescence intensity (RFU) induced by E2, indicating the enhanced-estrogenic activity of the three phthalates (Figure 5). DIDP and DNOP did not significantly affect (increase or decrease) the GFP signal intensity (RFU) induced by E2 (Figure 5), meaning these two phthalates are not estrogenic endocrine disrupting compounds.


Toxicity and estrogenic endocrine disrupting activity of phthalates and their mixtures.

Chen X, Xu S, Tan T, Lee ST, Cheng SH, Lee FW, Xu SJ, Ho KC - Int J Environ Res Public Health (2014)

Enhanced-estrogenic activity of phthalates. DBP (1.00 ppm), DEHP (1.50 ppm) and DINP (1.50 ppm) significantly increased, and DIDP (1.5 ppm) and DNOP (1.5 ppm) did not statistically increase or decrease, the GFP signal intensity (RFU) of the livers of transgenic medaka (O. melastigma) eleutheroembryos induced by E2 (2.5 ppb).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987027&req=5

ijerph-11-03156-f005: Enhanced-estrogenic activity of phthalates. DBP (1.00 ppm), DEHP (1.50 ppm) and DINP (1.50 ppm) significantly increased, and DIDP (1.5 ppm) and DNOP (1.5 ppm) did not statistically increase or decrease, the GFP signal intensity (RFU) of the livers of transgenic medaka (O. melastigma) eleutheroembryos induced by E2 (2.5 ppb).
Mentions: Results showed that 1.00 ppm DBP, 1.50 ppm DEHP or DINP significantly increased the green fluorescence intensity (RFU) induced by E2, indicating the enhanced-estrogenic activity of the three phthalates (Figure 5). DIDP and DNOP did not significantly affect (increase or decrease) the GFP signal intensity (RFU) induced by E2 (Figure 5), meaning these two phthalates are not estrogenic endocrine disrupting compounds.

Bottom Line: Phthalates, widely used in flexible plastics and consumer products, have become ubiquitous contaminants worldwide.The other four phthalates did not cause more than 50% exposed embryo mortality even at their highest soluble concentrations.Using an estrogen-responsive ChgH-EGFP transgenic medaka (Oryzias melastigma) eleutheroembryos based 24 h test, BBP demonstrated estrogenic activity, DBP, DEHP, DINP and the mixture of the six phthalates exhibited enhanced-estrogenic activity and DIDP and DNOP showed no enhanced- or anti-estrogenic activity.

View Article: PubMed Central - PubMed

Affiliation: Vitargent (International) Biotechnology Limited, Unit 516, 5/F. Biotech Centre 2, No. 11 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong. xueping.chen@hotmail.com.

ABSTRACT
Phthalates, widely used in flexible plastics and consumer products, have become ubiquitous contaminants worldwide. This study evaluated the acute toxicity and estrogenic endocrine disrupting activity of butyl benzyl phthalate (BBP), di(n-butyl) phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), di-n-octyl phthalate (DNOP) and their mixtures. Using a 72 h zebrafish embryo toxicity test, the LC50 values of BBP, DBP and a mixture of the six phthalates were found to be 0.72, 0.63 and 0.50 ppm, respectively. The other four phthalates did not cause more than 50% exposed embryo mortality even at their highest soluble concentrations. The typical toxicity symptoms caused by phthalates were death, tail curvature, necrosis, cardio edema and no touch response. Using an estrogen-responsive ChgH-EGFP transgenic medaka (Oryzias melastigma) eleutheroembryos based 24 h test, BBP demonstrated estrogenic activity, DBP, DEHP, DINP and the mixture of the six phthalates exhibited enhanced-estrogenic activity and DIDP and DNOP showed no enhanced- or anti-estrogenic activity. These findings highlighted the developmental toxicity of BBP and DBP, and the estrogenic endocrine disrupting activity of BBP, DBP, DEHP and DINP on intact organisms, indicating that the widespread use of these phthalates may cause potential health risks to human beings.

Show MeSH
Related in: MedlinePlus