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Influence of heavy metals and PCBs pollution on the enzyme activity and microbial community of paddy soils around an e-waste recycling workshop.

Tang X, Hashmi MZ, Long D, Chen L, Khan MI, Shen C - Int J Environ Res Public Health (2014)

Bottom Line: The results indicated that the heavy metal and PCB pollution did not differ significantly with an increase of the sampling point distances (5 to 30 m).However, a slight influence for soil microbial communities could be found based on DGGE, the Shannon index and PCA analysis.The present study suggests that the contamination stress of heavy metals and PCBs might have a slight influence on microbial activity in paddy soils.

View Article: PubMed Central - PubMed

Affiliation: College of Environmental and Natural Resource Sciences, Zhejiang University, Hangzhou 310058, China. xianjin@zju.edu.cn.

ABSTRACT
Due to the emerging environmental issues related to e-waste there is concern about the quality of paddy soils near e-waste workshops. The levels of heavy metals and PCBs and their influence on the enzyme activity and microbial community of paddy soils obtained from the immediate vicinity of an e-waste workshop were investigated in the present study. The results indicated that the heavy metal and PCB pollution did not differ significantly with an increase of the sampling point distances (5 to 30 m). The concentration of Cd (2.16 mg·kg-1) and Cu (69.2 mg·kg-1) were higher, and the PCB pollution was also serious, ranging from 4.9 to 21.6 μg·kg-1. The highest enzyme activity was found for urease compared to phosphatase and catalase, and a fluctuating trend in soil enzyme activity was observed in soils from different sampling sites. The microbial analysis revealed that there was no apparent correlation between the microbial community and the pollutants. However, a slight influence for soil microbial communities could be found based on DGGE, the Shannon index and PCA analysis. The present study suggests that the contamination stress of heavy metals and PCBs might have a slight influence on microbial activity in paddy soils. This study provides the baseline data for enzyme activities and microbial communities in paddy soil under the influence of mixed contamination.

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Map of study area and the six sampling points (P1–P6).
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ijerph-11-03118-f001: Map of study area and the six sampling points (P1–P6).

Mentions: A typical workshop, which was located in the Anrong village of Taizhou and was mostly used to dispose of scrap wires and cables, was selected in the present study. The e-waste activities have been going on in this workshop for over 30 years and the techniques used include manual dismantling of fractions, and open burning of wire and cable to recover metals, etc. Specially, there was no air or water pollution control measures in this workshop and the generated pollution was released directly into the surrounding environment. A paddy field close to the workshop, as shown in Figure 1, was used to collect the surface soil samples (depth of 0~15 cm). Six soil sampling sites (P1, P2, P3, P4, P5 and P6) were selected at 5 m intervals on a line out from the workshop location, each at a distance of 5, 10, 15, 20, 25 and 30 m from the workshop. At each sampling location, three subsamples (0–15 cm) were collected and pooled together to make one representative sample of about 1,000 g in size. Following the sampling, plants, stones and other items were carefully removed. Soil samples were then stored at 4 and −20 °C for further chemical and biological analysis [23].


Influence of heavy metals and PCBs pollution on the enzyme activity and microbial community of paddy soils around an e-waste recycling workshop.

Tang X, Hashmi MZ, Long D, Chen L, Khan MI, Shen C - Int J Environ Res Public Health (2014)

Map of study area and the six sampling points (P1–P6).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3987024&req=5

ijerph-11-03118-f001: Map of study area and the six sampling points (P1–P6).
Mentions: A typical workshop, which was located in the Anrong village of Taizhou and was mostly used to dispose of scrap wires and cables, was selected in the present study. The e-waste activities have been going on in this workshop for over 30 years and the techniques used include manual dismantling of fractions, and open burning of wire and cable to recover metals, etc. Specially, there was no air or water pollution control measures in this workshop and the generated pollution was released directly into the surrounding environment. A paddy field close to the workshop, as shown in Figure 1, was used to collect the surface soil samples (depth of 0~15 cm). Six soil sampling sites (P1, P2, P3, P4, P5 and P6) were selected at 5 m intervals on a line out from the workshop location, each at a distance of 5, 10, 15, 20, 25 and 30 m from the workshop. At each sampling location, three subsamples (0–15 cm) were collected and pooled together to make one representative sample of about 1,000 g in size. Following the sampling, plants, stones and other items were carefully removed. Soil samples were then stored at 4 and −20 °C for further chemical and biological analysis [23].

Bottom Line: The results indicated that the heavy metal and PCB pollution did not differ significantly with an increase of the sampling point distances (5 to 30 m).However, a slight influence for soil microbial communities could be found based on DGGE, the Shannon index and PCA analysis.The present study suggests that the contamination stress of heavy metals and PCBs might have a slight influence on microbial activity in paddy soils.

View Article: PubMed Central - PubMed

Affiliation: College of Environmental and Natural Resource Sciences, Zhejiang University, Hangzhou 310058, China. xianjin@zju.edu.cn.

ABSTRACT
Due to the emerging environmental issues related to e-waste there is concern about the quality of paddy soils near e-waste workshops. The levels of heavy metals and PCBs and their influence on the enzyme activity and microbial community of paddy soils obtained from the immediate vicinity of an e-waste workshop were investigated in the present study. The results indicated that the heavy metal and PCB pollution did not differ significantly with an increase of the sampling point distances (5 to 30 m). The concentration of Cd (2.16 mg·kg-1) and Cu (69.2 mg·kg-1) were higher, and the PCB pollution was also serious, ranging from 4.9 to 21.6 μg·kg-1. The highest enzyme activity was found for urease compared to phosphatase and catalase, and a fluctuating trend in soil enzyme activity was observed in soils from different sampling sites. The microbial analysis revealed that there was no apparent correlation between the microbial community and the pollutants. However, a slight influence for soil microbial communities could be found based on DGGE, the Shannon index and PCA analysis. The present study suggests that the contamination stress of heavy metals and PCBs might have a slight influence on microbial activity in paddy soils. This study provides the baseline data for enzyme activities and microbial communities in paddy soil under the influence of mixed contamination.

Show MeSH