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PAR-3 knockdown enhances adhesion rate of PANC-1 cells via increased expression of integrinαv and E-cadherin.

Segal L, Katz LS, Shapira H, Sandbank J, Geras-Raaka E, Gershengorn MC, Oron Y - PLoS ONE (2014)

Bottom Line: ITGαv, as well as ITGα6 and ITGα10 mRNAs, were greatly enriched (>40-fold) in a rapidly-adhering sub-population of PAR-3 KD cells.However, ITGαv mRNA and protein expression was increased in PAR-3 KD and markedly decreased in PAR-1 KD.PAR-3 KD cells also expressed more E-cadherin mRNA and protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.

ABSTRACT
The balance between the adhesion of cancer cells to extracellular matrix and their migratory potential, as well as their proteolytic activity, are important parameters that determine cancer cells invasiveness and metastasis. Since thrombin has been implicated in cancer progression, we studied the role(s) of thrombin-activated receptors in the adhesion process. We stably knocked down proteinase-activated receptors (PARs) -1, or -3 in human pancreatic adenocarcinoma PANC-1 cells. PANC-1 cells exhibit rapid adhesion to cell culture treated plastic and much faster kinetics of adhesion to Matrigel coated surface. Knockdown of PAR-1 had no effect on cells' adhesiveness, while PAR-3 knockdowns (KDs) exhibited much faster adhesion kinetics. PAR-3 KDs also exhibited slower in vitro wound closure than vector-control and PAR-1 KD cells. To study the molecular mechanism(s) of PAR-3 KD cells' enhanced rate of adhesion, we assayed the expression of the molecules that mediate cell-surface and cell-cell adhesion. ITGαv, as well as ITGα6 and ITGα10 mRNAs, were greatly enriched (>40-fold) in a rapidly-adhering sub-population of PAR-3 KD cells. The whole population of both PAR-1 and -3 KDs exhibited enhanced expression of a number of integrins (ITGs) mRNAs. However, ITGαv mRNA and protein expression was increased in PAR-3 KD and markedly decreased in PAR-1 KD. PAR-3 KD cells also expressed more E-cadherin mRNA and protein. The enhanced adhesion kinetics of PAR-3 KDs was almost fully inhibited by calcium chelation, or by a HAV-motive decapeptide that affects E-cadherin intermolecular interactions. We propose that the enhanced rate of adhesion of PAR-3 KDs results from enhanced expression of E-cadherin, leading to a greater adhesion of free-floating cells to cells rapidly bound to the surface via their integrins, and particularly ITGαv.

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The effect of E-cadherin inhibition on adhesion.Vector-control or PAR-3 KD cells were plated in 96-wells clusters at 25,000 cells/well and allowed to adhere for 30 min. EGTA (5 mM) or HAV inhibitor peptide (1 mM) were added at time 0.
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pone-0093879-g007: The effect of E-cadherin inhibition on adhesion.Vector-control or PAR-3 KD cells were plated in 96-wells clusters at 25,000 cells/well and allowed to adhere for 30 min. EGTA (5 mM) or HAV inhibitor peptide (1 mM) were added at time 0.

Mentions: To test the hypothesis that the increased expression of E-cadherin was responsible for the more rapid kinetics of PAR-3 KDs adhesion, we included in the adhesion assay either 2.5 mM EGTA (to chelate calcium ions), or 1 mM of the HAV-motive decapeptide (previously shown to inhibit E-cadherin inter-molecular interactions, [14]). Both treatments greatly reduced the rapid adhesion of PAR-3 KD cells and had no effect on the adhesion of vector-controls (Fig. 7).


PAR-3 knockdown enhances adhesion rate of PANC-1 cells via increased expression of integrinαv and E-cadherin.

Segal L, Katz LS, Shapira H, Sandbank J, Geras-Raaka E, Gershengorn MC, Oron Y - PLoS ONE (2014)

The effect of E-cadherin inhibition on adhesion.Vector-control or PAR-3 KD cells were plated in 96-wells clusters at 25,000 cells/well and allowed to adhere for 30 min. EGTA (5 mM) or HAV inhibitor peptide (1 mM) were added at time 0.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3974847&req=5

pone-0093879-g007: The effect of E-cadherin inhibition on adhesion.Vector-control or PAR-3 KD cells were plated in 96-wells clusters at 25,000 cells/well and allowed to adhere for 30 min. EGTA (5 mM) or HAV inhibitor peptide (1 mM) were added at time 0.
Mentions: To test the hypothesis that the increased expression of E-cadherin was responsible for the more rapid kinetics of PAR-3 KDs adhesion, we included in the adhesion assay either 2.5 mM EGTA (to chelate calcium ions), or 1 mM of the HAV-motive decapeptide (previously shown to inhibit E-cadherin inter-molecular interactions, [14]). Both treatments greatly reduced the rapid adhesion of PAR-3 KD cells and had no effect on the adhesion of vector-controls (Fig. 7).

Bottom Line: ITGαv, as well as ITGα6 and ITGα10 mRNAs, were greatly enriched (>40-fold) in a rapidly-adhering sub-population of PAR-3 KD cells.However, ITGαv mRNA and protein expression was increased in PAR-3 KD and markedly decreased in PAR-1 KD.PAR-3 KD cells also expressed more E-cadherin mRNA and protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.

ABSTRACT
The balance between the adhesion of cancer cells to extracellular matrix and their migratory potential, as well as their proteolytic activity, are important parameters that determine cancer cells invasiveness and metastasis. Since thrombin has been implicated in cancer progression, we studied the role(s) of thrombin-activated receptors in the adhesion process. We stably knocked down proteinase-activated receptors (PARs) -1, or -3 in human pancreatic adenocarcinoma PANC-1 cells. PANC-1 cells exhibit rapid adhesion to cell culture treated plastic and much faster kinetics of adhesion to Matrigel coated surface. Knockdown of PAR-1 had no effect on cells' adhesiveness, while PAR-3 knockdowns (KDs) exhibited much faster adhesion kinetics. PAR-3 KDs also exhibited slower in vitro wound closure than vector-control and PAR-1 KD cells. To study the molecular mechanism(s) of PAR-3 KD cells' enhanced rate of adhesion, we assayed the expression of the molecules that mediate cell-surface and cell-cell adhesion. ITGαv, as well as ITGα6 and ITGα10 mRNAs, were greatly enriched (>40-fold) in a rapidly-adhering sub-population of PAR-3 KD cells. The whole population of both PAR-1 and -3 KDs exhibited enhanced expression of a number of integrins (ITGs) mRNAs. However, ITGαv mRNA and protein expression was increased in PAR-3 KD and markedly decreased in PAR-1 KD. PAR-3 KD cells also expressed more E-cadherin mRNA and protein. The enhanced adhesion kinetics of PAR-3 KDs was almost fully inhibited by calcium chelation, or by a HAV-motive decapeptide that affects E-cadherin intermolecular interactions. We propose that the enhanced rate of adhesion of PAR-3 KDs results from enhanced expression of E-cadherin, leading to a greater adhesion of free-floating cells to cells rapidly bound to the surface via their integrins, and particularly ITGαv.

Show MeSH
Related in: MedlinePlus