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The expression and significance of neuronal iconic proteins in podocytes.

Sun Y, Zhang H, Hu R, Sun J, Mao X, Zhao Z, Chen Q, Zhang Z - PLoS ONE (2014)

Bottom Line: When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time.Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins.The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Molecular Medicine, Chinese Ministry of Education, Shanghai Medical College, School of Basic Medical Science, Fudan University, Shanghai, P.R. China.

ABSTRACT
Growing evidence suggests that there are many common cell biological features shared by neurons and podocytes; however, the mechanism of podocyte foot process formation remains unclear. Comparing the mechanisms of process formation between two cell types should provide useful guidance from the progress of neuron research. Studies have shown that some mature proteins of podocytes, such as podocin, nephrin, and synaptopodin, were also expressed in neurons. In this study, using cell biological experiments and immunohistochemical techniques, we showed that some neuronal iconic molecules, such as Neuron-specific enolase, nestin and Neuron-specific nuclear protein, were also expressed in podocytes. We further inhibited the expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 by Small interfering RNA in cultured mouse podocytes and observed the significant morphological changes in treated podocytes. When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time. Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins. The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

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The morphologic changes of podocytes that were treated with Adriamycin.The podocytes were treated with Adriamycin (20 μg/ml) for different times. The expression levels of nestin, NSE synaptopodin and UCH-L1 continued to decrease in correlation with the stimulation time (A). The statistical results show significant differences between the controlled and treated groups (B) (compared with. control, *p<0.001). Two hours after treating the cultured mouse podocytes with Adriamycin (20 μg/ml), the cytoskeletons changed significantly, and as the stimulus time increased, the destruction of the cytoskeleton and morphology became more severe (C). The data are presented from at least three individual experiments that were performed in duplicate. Fluorescence Phallotoxins ×400.
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pone-0093999-g005: The morphologic changes of podocytes that were treated with Adriamycin.The podocytes were treated with Adriamycin (20 μg/ml) for different times. The expression levels of nestin, NSE synaptopodin and UCH-L1 continued to decrease in correlation with the stimulation time (A). The statistical results show significant differences between the controlled and treated groups (B) (compared with. control, *p<0.001). Two hours after treating the cultured mouse podocytes with Adriamycin (20 μg/ml), the cytoskeletons changed significantly, and as the stimulus time increased, the destruction of the cytoskeleton and morphology became more severe (C). The data are presented from at least three individual experiments that were performed in duplicate. Fluorescence Phallotoxins ×400.

Mentions: To further investigate the changes of these neuronal iconic proteins in injured podocytes, we treated the cultured mouse podocytes with Adriamycin (20 μg/ml) for various periods. The results showed that the podocyte morphology did not change significantly when stimulated for 30 min and 1 h; however, the podocytes became swollen as actin fibers became disordered when the stimulation lasted for 2 hours. When the stimulus lasted more than 2 hours, the disordered arrangement of the cytoskeleton became more severe (Fig. 5C). Meanwhile, the Western blotting showed that the decrease in the protein expression of nestin, NSE, synaptopodin and UCH-L1 was dependent on the time of stimulation (Fig. 5A). These results indicate that when podocytes were damaged, the expression of these neuronal iconic proteins were also affected, which resulted in disordered cytoskeleton, changes in cell morphology, clinical proteinuria and other symptoms. These data further proved that neuronal iconic proteins might play an important role in maintaining the special morphology and functions of podocytes, as well as in the pathogenesis of podocyte injury.


The expression and significance of neuronal iconic proteins in podocytes.

Sun Y, Zhang H, Hu R, Sun J, Mao X, Zhao Z, Chen Q, Zhang Z - PLoS ONE (2014)

The morphologic changes of podocytes that were treated with Adriamycin.The podocytes were treated with Adriamycin (20 μg/ml) for different times. The expression levels of nestin, NSE synaptopodin and UCH-L1 continued to decrease in correlation with the stimulation time (A). The statistical results show significant differences between the controlled and treated groups (B) (compared with. control, *p<0.001). Two hours after treating the cultured mouse podocytes with Adriamycin (20 μg/ml), the cytoskeletons changed significantly, and as the stimulus time increased, the destruction of the cytoskeleton and morphology became more severe (C). The data are presented from at least three individual experiments that were performed in duplicate. Fluorescence Phallotoxins ×400.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974844&req=5

pone-0093999-g005: The morphologic changes of podocytes that were treated with Adriamycin.The podocytes were treated with Adriamycin (20 μg/ml) for different times. The expression levels of nestin, NSE synaptopodin and UCH-L1 continued to decrease in correlation with the stimulation time (A). The statistical results show significant differences between the controlled and treated groups (B) (compared with. control, *p<0.001). Two hours after treating the cultured mouse podocytes with Adriamycin (20 μg/ml), the cytoskeletons changed significantly, and as the stimulus time increased, the destruction of the cytoskeleton and morphology became more severe (C). The data are presented from at least three individual experiments that were performed in duplicate. Fluorescence Phallotoxins ×400.
Mentions: To further investigate the changes of these neuronal iconic proteins in injured podocytes, we treated the cultured mouse podocytes with Adriamycin (20 μg/ml) for various periods. The results showed that the podocyte morphology did not change significantly when stimulated for 30 min and 1 h; however, the podocytes became swollen as actin fibers became disordered when the stimulation lasted for 2 hours. When the stimulus lasted more than 2 hours, the disordered arrangement of the cytoskeleton became more severe (Fig. 5C). Meanwhile, the Western blotting showed that the decrease in the protein expression of nestin, NSE, synaptopodin and UCH-L1 was dependent on the time of stimulation (Fig. 5A). These results indicate that when podocytes were damaged, the expression of these neuronal iconic proteins were also affected, which resulted in disordered cytoskeleton, changes in cell morphology, clinical proteinuria and other symptoms. These data further proved that neuronal iconic proteins might play an important role in maintaining the special morphology and functions of podocytes, as well as in the pathogenesis of podocyte injury.

Bottom Line: When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time.Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins.The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Molecular Medicine, Chinese Ministry of Education, Shanghai Medical College, School of Basic Medical Science, Fudan University, Shanghai, P.R. China.

ABSTRACT
Growing evidence suggests that there are many common cell biological features shared by neurons and podocytes; however, the mechanism of podocyte foot process formation remains unclear. Comparing the mechanisms of process formation between two cell types should provide useful guidance from the progress of neuron research. Studies have shown that some mature proteins of podocytes, such as podocin, nephrin, and synaptopodin, were also expressed in neurons. In this study, using cell biological experiments and immunohistochemical techniques, we showed that some neuronal iconic molecules, such as Neuron-specific enolase, nestin and Neuron-specific nuclear protein, were also expressed in podocytes. We further inhibited the expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 by Small interfering RNA in cultured mouse podocytes and observed the significant morphological changes in treated podocytes. When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time. Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins. The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

Show MeSH
Related in: MedlinePlus