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The expression and significance of neuronal iconic proteins in podocytes.

Sun Y, Zhang H, Hu R, Sun J, Mao X, Zhao Z, Chen Q, Zhang Z - PLoS ONE (2014)

Bottom Line: When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time.Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins.The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Molecular Medicine, Chinese Ministry of Education, Shanghai Medical College, School of Basic Medical Science, Fudan University, Shanghai, P.R. China.

ABSTRACT
Growing evidence suggests that there are many common cell biological features shared by neurons and podocytes; however, the mechanism of podocyte foot process formation remains unclear. Comparing the mechanisms of process formation between two cell types should provide useful guidance from the progress of neuron research. Studies have shown that some mature proteins of podocytes, such as podocin, nephrin, and synaptopodin, were also expressed in neurons. In this study, using cell biological experiments and immunohistochemical techniques, we showed that some neuronal iconic molecules, such as Neuron-specific enolase, nestin and Neuron-specific nuclear protein, were also expressed in podocytes. We further inhibited the expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 by Small interfering RNA in cultured mouse podocytes and observed the significant morphological changes in treated podocytes. When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time. Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins. The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

Show MeSH
Neuronal iconic proteins that are expressed in mouse podocytes and brain neurons.Neuronal iconic proteins NSE (47 kDa), NeuN (38 kDa), nestin (200 kDa), S100 (21 kDa), nephrin (84 kDa), synaptopodin (44 kDa in podocyte, 100 kDa in neuron), UCH-L1 (27 kDa) and α-actinin-4 (100 kDa) were detected by Western blotting in both podocytes and brain neurons. The data are presented from at least three individual experiments that were performed in duplicate.
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pone-0093999-g002: Neuronal iconic proteins that are expressed in mouse podocytes and brain neurons.Neuronal iconic proteins NSE (47 kDa), NeuN (38 kDa), nestin (200 kDa), S100 (21 kDa), nephrin (84 kDa), synaptopodin (44 kDa in podocyte, 100 kDa in neuron), UCH-L1 (27 kDa) and α-actinin-4 (100 kDa) were detected by Western blotting in both podocytes and brain neurons. The data are presented from at least three individual experiments that were performed in duplicate.

Mentions: We extracted total proteins from cultured mouse podocytes and rat neurons. Western blotting detected the same proteins in neurons and podocytes as abovementioned. The results indicated that all of these proteins were expressed in both podocytes and brain neurons (Fig. 2). The molecular weights of NSE, NeuN, nestin and S100, as well as nephrin, UCH-L1 and α-actinin-4, were of the same size in both podocytes and neurons, whereas synaptopodin is 44 kDa in podocytes and 100 kDa in neurons, which is consistent with Dr. Mundel's report [7].


The expression and significance of neuronal iconic proteins in podocytes.

Sun Y, Zhang H, Hu R, Sun J, Mao X, Zhao Z, Chen Q, Zhang Z - PLoS ONE (2014)

Neuronal iconic proteins that are expressed in mouse podocytes and brain neurons.Neuronal iconic proteins NSE (47 kDa), NeuN (38 kDa), nestin (200 kDa), S100 (21 kDa), nephrin (84 kDa), synaptopodin (44 kDa in podocyte, 100 kDa in neuron), UCH-L1 (27 kDa) and α-actinin-4 (100 kDa) were detected by Western blotting in both podocytes and brain neurons. The data are presented from at least three individual experiments that were performed in duplicate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974844&req=5

pone-0093999-g002: Neuronal iconic proteins that are expressed in mouse podocytes and brain neurons.Neuronal iconic proteins NSE (47 kDa), NeuN (38 kDa), nestin (200 kDa), S100 (21 kDa), nephrin (84 kDa), synaptopodin (44 kDa in podocyte, 100 kDa in neuron), UCH-L1 (27 kDa) and α-actinin-4 (100 kDa) were detected by Western blotting in both podocytes and brain neurons. The data are presented from at least three individual experiments that were performed in duplicate.
Mentions: We extracted total proteins from cultured mouse podocytes and rat neurons. Western blotting detected the same proteins in neurons and podocytes as abovementioned. The results indicated that all of these proteins were expressed in both podocytes and brain neurons (Fig. 2). The molecular weights of NSE, NeuN, nestin and S100, as well as nephrin, UCH-L1 and α-actinin-4, were of the same size in both podocytes and neurons, whereas synaptopodin is 44 kDa in podocytes and 100 kDa in neurons, which is consistent with Dr. Mundel's report [7].

Bottom Line: When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time.Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins.The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Molecular Medicine, Chinese Ministry of Education, Shanghai Medical College, School of Basic Medical Science, Fudan University, Shanghai, P.R. China.

ABSTRACT
Growing evidence suggests that there are many common cell biological features shared by neurons and podocytes; however, the mechanism of podocyte foot process formation remains unclear. Comparing the mechanisms of process formation between two cell types should provide useful guidance from the progress of neuron research. Studies have shown that some mature proteins of podocytes, such as podocin, nephrin, and synaptopodin, were also expressed in neurons. In this study, using cell biological experiments and immunohistochemical techniques, we showed that some neuronal iconic molecules, such as Neuron-specific enolase, nestin and Neuron-specific nuclear protein, were also expressed in podocytes. We further inhibited the expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 by Small interfering RNA in cultured mouse podocytes and observed the significant morphological changes in treated podocytes. When podocytes were treated with Adriamycin, the protein expression of Neuron-specific enolase, nestin, synaptopodin and Ubiquitin carboxy terminal hydrolase-1 decreased over time. Meanwhile, the morphological changes in the podocytes were consistent with results of the Small interfering RNA treatment of these proteins. The data demonstrated that neuronal iconic proteins play important roles in maintaining and regulating the formation and function of podocyte processes.

Show MeSH