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Automated analyses of innate olfactory behaviors in rodents.

Qiu Q, Scott A, Scheerer H, Sapkota N, Lee DK, Ma L, Yu CR - PLoS ONE (2014)

Bottom Line: Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation.In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays.Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays.

View Article: PubMed Central - PubMed

Affiliation: Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.

ABSTRACT
Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation. The predominant experimental paradigms employ forced choice operant assays, which require associative learning and reinforced training. Animal performance in these assays not only reflects odor perception but also the confidence in decision making and memory. In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays. In addition to forced choice assays, we employ this system to examine animal's innate ability for odor detection, discrimination and preference without elaborate training procedures. These assays provide quantitative measurements of odor discrimination and robust readouts of odor preference. Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays. PROBES-based automated assays provide an efficient way of analyzing innate odor-triggered behaviors.

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PROBES based habituation/Dis-habituation Analysis of Innate Odor Preference.A&B) Bar plots of odor port investigation by a single animal (upper panel) and the average of NPI value for FU (A, 10 animals) or 2-MBA (B, 11 animals). Dashed lines indicate 100% of average NPI to mineral oil. Calculation of attraction and aversion indexes is indicated. C&D) Comparison of attraction(C) and aversion (D) indexes obtained by PROBES assay and 3-chamber assays. The p values of the tests are indicated. E) Comparison table of PROBES and 3-chamber methods for innate preference assays.
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pone-0093468-g009: PROBES based habituation/Dis-habituation Analysis of Innate Odor Preference.A&B) Bar plots of odor port investigation by a single animal (upper panel) and the average of NPI value for FU (A, 10 animals) or 2-MBA (B, 11 animals). Dashed lines indicate 100% of average NPI to mineral oil. Calculation of attraction and aversion indexes is indicated. C&D) Comparison of attraction(C) and aversion (D) indexes obtained by PROBES assay and 3-chamber assays. The p values of the tests are indicated. E) Comparison table of PROBES and 3-chamber methods for innate preference assays.

Mentions: We reasoned that the inherent variability in the three-chamber assay degraded the signal. We then explored the possibility of using a single chamber PROBES to examine the innate odor preference in a habituation/dis-habituation assay (Figure 9). Presentation of mouse urine elicited a significant increase in investigation (Figure 9A). In contrast, 2-MBA incited only a slight increase in investigation when first presented. Surprisingly, in subsequent 2-MBA sessions, the mice appeared to avoid approaching the odor port (Figure 9B). These observations indicated that ΔNPI value could be used to evaluate both attraction and aversion. Therefore, we calculated the Attraction Index by subtracting the NPI of last trial of mineral oil from the first trial of test odor (Figure 9A). Because aversion was better represented by the ΔNPI between the second odor presentation and final mineral oil control (Figure 9B), it was used as Aversion Index.


Automated analyses of innate olfactory behaviors in rodents.

Qiu Q, Scott A, Scheerer H, Sapkota N, Lee DK, Ma L, Yu CR - PLoS ONE (2014)

PROBES based habituation/Dis-habituation Analysis of Innate Odor Preference.A&B) Bar plots of odor port investigation by a single animal (upper panel) and the average of NPI value for FU (A, 10 animals) or 2-MBA (B, 11 animals). Dashed lines indicate 100% of average NPI to mineral oil. Calculation of attraction and aversion indexes is indicated. C&D) Comparison of attraction(C) and aversion (D) indexes obtained by PROBES assay and 3-chamber assays. The p values of the tests are indicated. E) Comparison table of PROBES and 3-chamber methods for innate preference assays.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974772&req=5

pone-0093468-g009: PROBES based habituation/Dis-habituation Analysis of Innate Odor Preference.A&B) Bar plots of odor port investigation by a single animal (upper panel) and the average of NPI value for FU (A, 10 animals) or 2-MBA (B, 11 animals). Dashed lines indicate 100% of average NPI to mineral oil. Calculation of attraction and aversion indexes is indicated. C&D) Comparison of attraction(C) and aversion (D) indexes obtained by PROBES assay and 3-chamber assays. The p values of the tests are indicated. E) Comparison table of PROBES and 3-chamber methods for innate preference assays.
Mentions: We reasoned that the inherent variability in the three-chamber assay degraded the signal. We then explored the possibility of using a single chamber PROBES to examine the innate odor preference in a habituation/dis-habituation assay (Figure 9). Presentation of mouse urine elicited a significant increase in investigation (Figure 9A). In contrast, 2-MBA incited only a slight increase in investigation when first presented. Surprisingly, in subsequent 2-MBA sessions, the mice appeared to avoid approaching the odor port (Figure 9B). These observations indicated that ΔNPI value could be used to evaluate both attraction and aversion. Therefore, we calculated the Attraction Index by subtracting the NPI of last trial of mineral oil from the first trial of test odor (Figure 9A). Because aversion was better represented by the ΔNPI between the second odor presentation and final mineral oil control (Figure 9B), it was used as Aversion Index.

Bottom Line: Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation.In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays.Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays.

View Article: PubMed Central - PubMed

Affiliation: Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.

ABSTRACT
Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation. The predominant experimental paradigms employ forced choice operant assays, which require associative learning and reinforced training. Animal performance in these assays not only reflects odor perception but also the confidence in decision making and memory. In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays. In addition to forced choice assays, we employ this system to examine animal's innate ability for odor detection, discrimination and preference without elaborate training procedures. These assays provide quantitative measurements of odor discrimination and robust readouts of odor preference. Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays. PROBES-based automated assays provide an efficient way of analyzing innate odor-triggered behaviors.

Show MeSH