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Automated analyses of innate olfactory behaviors in rodents.

Qiu Q, Scott A, Scheerer H, Sapkota N, Lee DK, Ma L, Yu CR - PLoS ONE (2014)

Bottom Line: Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation.In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays.Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays.

View Article: PubMed Central - PubMed

Affiliation: Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.

ABSTRACT
Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation. The predominant experimental paradigms employ forced choice operant assays, which require associative learning and reinforced training. Animal performance in these assays not only reflects odor perception but also the confidence in decision making and memory. In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays. In addition to forced choice assays, we employ this system to examine animal's innate ability for odor detection, discrimination and preference without elaborate training procedures. These assays provide quantitative measurements of odor discrimination and robust readouts of odor preference. Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays. PROBES-based automated assays provide an efficient way of analyzing innate odor-triggered behaviors.

Show MeSH
Behavior Box Design.A) Single port design with one pair IR LED emitter and detector that can be used to trigger odor delivery. B) A triple port design that combines the odor port in A with two water ports. A water spout is included in each water port. C) A single port design that combines water and odor delivery. Two sets of paired IR LED emitter and detector are implemented to detect nose poking and licking activity.
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pone-0093468-g002: Behavior Box Design.A) Single port design with one pair IR LED emitter and detector that can be used to trigger odor delivery. B) A triple port design that combines the odor port in A with two water ports. A water spout is included in each water port. C) A single port design that combines water and odor delivery. Two sets of paired IR LED emitter and detector are implemented to detect nose poking and licking activity.

Mentions: The behavioral chamber is a simple 20 cm×20 cm×20 cm acrylic box with a interchangeable front panel that can house up to three ports for different behavioral tasks (Figures 1D & 2). The back panel contains a vacuum port to facilitate air exchange inside the chamber. We implemented three configurations to accommodate different behavioral tasks. The first one, shown in Figure 2A, utilizes a port located in the center with a single paired infrared light emitting diode (IR-LED) and IR detector installed. Beam-breaking events indicate nose poking by the animal (Figure 2A). This simple implementation can be used for odor threshold detection, cross habituation and odor preference evaluation.


Automated analyses of innate olfactory behaviors in rodents.

Qiu Q, Scott A, Scheerer H, Sapkota N, Lee DK, Ma L, Yu CR - PLoS ONE (2014)

Behavior Box Design.A) Single port design with one pair IR LED emitter and detector that can be used to trigger odor delivery. B) A triple port design that combines the odor port in A with two water ports. A water spout is included in each water port. C) A single port design that combines water and odor delivery. Two sets of paired IR LED emitter and detector are implemented to detect nose poking and licking activity.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974772&req=5

pone-0093468-g002: Behavior Box Design.A) Single port design with one pair IR LED emitter and detector that can be used to trigger odor delivery. B) A triple port design that combines the odor port in A with two water ports. A water spout is included in each water port. C) A single port design that combines water and odor delivery. Two sets of paired IR LED emitter and detector are implemented to detect nose poking and licking activity.
Mentions: The behavioral chamber is a simple 20 cm×20 cm×20 cm acrylic box with a interchangeable front panel that can house up to three ports for different behavioral tasks (Figures 1D & 2). The back panel contains a vacuum port to facilitate air exchange inside the chamber. We implemented three configurations to accommodate different behavioral tasks. The first one, shown in Figure 2A, utilizes a port located in the center with a single paired infrared light emitting diode (IR-LED) and IR detector installed. Beam-breaking events indicate nose poking by the animal (Figure 2A). This simple implementation can be used for odor threshold detection, cross habituation and odor preference evaluation.

Bottom Line: Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation.In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays.Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays.

View Article: PubMed Central - PubMed

Affiliation: Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.

ABSTRACT
Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation. The predominant experimental paradigms employ forced choice operant assays, which require associative learning and reinforced training. Animal performance in these assays not only reflects odor perception but also the confidence in decision making and memory. In this study, we describe a versatile and automated setup, "Poking-Registered Olfactory Behavior Evaluation System" (PROBES), which can be adapted to perform multiple olfactory assays. In addition to forced choice assays, we employ this system to examine animal's innate ability for odor detection, discrimination and preference without elaborate training procedures. These assays provide quantitative measurements of odor discrimination and robust readouts of odor preference. Using PROBES, we find odor detection thresholds are at lower concentrations in naïve animals than those determined by forced choice assays. PROBES-based automated assays provide an efficient way of analyzing innate odor-triggered behaviors.

Show MeSH