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Characterization of fecal microbiota across seven Chinese ethnic groups by quantitative polymerase chain reaction.

Kwok LY, Zhang J, Guo Z, Gesudu Q, Zheng Y, Qiao J, Huo D, Zhang H - PLoS ONE (2014)

Bottom Line: Our data confirmed that the selected bacterial groups were common to all 7 surveyed ethnicities, but the amount of the individual bacterial groups varied to different degree.Nevertheless, weak differences were noted between the Han and Zhuang from other ethnic minority groups, and between the Heilongjiang Hans from those of the other provinces.Thus, our results suggest that the ethnic origin may contribute to shaping the human gut microbiota.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, P. R. China.

ABSTRACT
The human gut microbiota consists of complex microbial communities, which possibly play crucial roles in physiological functioning and health maintenance. China has evolved into a multicultural society consisting of the major ethnic group, Han, and 55 official ethnic minority groups. Nowadays, these minority groups inhabit in different Chinese provinces and some of them still keep their unique culture and lifestyle. Currently, only limited data are available on the gut microbiota of these Chinese ethnic groups. In this study, 10 major fecal bacterial groups of 314 healthy individuals from 7 Chinese ethnic origins were enumerated by quantitative polymerase chain reaction. Our data confirmed that the selected bacterial groups were common to all 7 surveyed ethnicities, but the amount of the individual bacterial groups varied to different degree. By principal component and canonical variate analyses of the 314 individuals or the 91 Han subjects, no distinct group clustering pattern was observed. Nevertheless, weak differences were noted between the Han and Zhuang from other ethnic minority groups, and between the Heilongjiang Hans from those of the other provinces. Thus, our results suggest that the ethnic origin may contribute to shaping the human gut microbiota.

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Variation of the fecal bacterial composition across the 7 ethnic groups.A. PCA score plot of the first two principal components with the proportion of variance explained by each component printed next to the axes labels. B. MANOVA score plot using the first 9 principal components. Individuals are classified according to their corresponding ethnic group and lifestyle. The first two canonical variables are plotted. Each individual is represented by one dot and the color label corresponds to the ethnic origin and lifestyle. C. Dendrogram constructed based on the distance metrics of different ethnic groups. ‘*’ and ‘***’ indicate p<0.05, 0.001, respectively.
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pone-0093631-g003: Variation of the fecal bacterial composition across the 7 ethnic groups.A. PCA score plot of the first two principal components with the proportion of variance explained by each component printed next to the axes labels. B. MANOVA score plot using the first 9 principal components. Individuals are classified according to their corresponding ethnic group and lifestyle. The first two canonical variables are plotted. Each individual is represented by one dot and the color label corresponds to the ethnic origin and lifestyle. C. Dendrogram constructed based on the distance metrics of different ethnic groups. ‘*’ and ‘***’ indicate p<0.05, 0.001, respectively.

Mentions: PCA was used to display any clustering pattern of fecal bacteria of the 314 individuals. No distinct grouping was observed within any ethnic group (Figure 3A). MANOVA was further performed to analyze the variability of the 314 samples. Similarly, no distinct grouping was observed. However, there was a tendency of accumulation of the Tibetan and Mongolian samples at the right quadrants of the score plot, whereas most of the Zhuang and Han samples were located at the left quadrants (Figure 3B), suggesting a mild difference of the Tibetan and Mongolian samples from those of the Zhuang and Han. Results from the cluster analysis supported such difference (Figure 3C and Figure S2). Samples from the Han and Zhuang formed a distinct cluster separated from the other ethnic groups. The Bai, Kazakh and Uyghur (urban-dwelling) grouped together. The Tibetan and Mongolian (rural-dwelling) were closer to each other than to other ethnic groups.


Characterization of fecal microbiota across seven Chinese ethnic groups by quantitative polymerase chain reaction.

Kwok LY, Zhang J, Guo Z, Gesudu Q, Zheng Y, Qiao J, Huo D, Zhang H - PLoS ONE (2014)

Variation of the fecal bacterial composition across the 7 ethnic groups.A. PCA score plot of the first two principal components with the proportion of variance explained by each component printed next to the axes labels. B. MANOVA score plot using the first 9 principal components. Individuals are classified according to their corresponding ethnic group and lifestyle. The first two canonical variables are plotted. Each individual is represented by one dot and the color label corresponds to the ethnic origin and lifestyle. C. Dendrogram constructed based on the distance metrics of different ethnic groups. ‘*’ and ‘***’ indicate p<0.05, 0.001, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974763&req=5

pone-0093631-g003: Variation of the fecal bacterial composition across the 7 ethnic groups.A. PCA score plot of the first two principal components with the proportion of variance explained by each component printed next to the axes labels. B. MANOVA score plot using the first 9 principal components. Individuals are classified according to their corresponding ethnic group and lifestyle. The first two canonical variables are plotted. Each individual is represented by one dot and the color label corresponds to the ethnic origin and lifestyle. C. Dendrogram constructed based on the distance metrics of different ethnic groups. ‘*’ and ‘***’ indicate p<0.05, 0.001, respectively.
Mentions: PCA was used to display any clustering pattern of fecal bacteria of the 314 individuals. No distinct grouping was observed within any ethnic group (Figure 3A). MANOVA was further performed to analyze the variability of the 314 samples. Similarly, no distinct grouping was observed. However, there was a tendency of accumulation of the Tibetan and Mongolian samples at the right quadrants of the score plot, whereas most of the Zhuang and Han samples were located at the left quadrants (Figure 3B), suggesting a mild difference of the Tibetan and Mongolian samples from those of the Zhuang and Han. Results from the cluster analysis supported such difference (Figure 3C and Figure S2). Samples from the Han and Zhuang formed a distinct cluster separated from the other ethnic groups. The Bai, Kazakh and Uyghur (urban-dwelling) grouped together. The Tibetan and Mongolian (rural-dwelling) were closer to each other than to other ethnic groups.

Bottom Line: Our data confirmed that the selected bacterial groups were common to all 7 surveyed ethnicities, but the amount of the individual bacterial groups varied to different degree.Nevertheless, weak differences were noted between the Han and Zhuang from other ethnic minority groups, and between the Heilongjiang Hans from those of the other provinces.Thus, our results suggest that the ethnic origin may contribute to shaping the human gut microbiota.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, P. R. China.

ABSTRACT
The human gut microbiota consists of complex microbial communities, which possibly play crucial roles in physiological functioning and health maintenance. China has evolved into a multicultural society consisting of the major ethnic group, Han, and 55 official ethnic minority groups. Nowadays, these minority groups inhabit in different Chinese provinces and some of them still keep their unique culture and lifestyle. Currently, only limited data are available on the gut microbiota of these Chinese ethnic groups. In this study, 10 major fecal bacterial groups of 314 healthy individuals from 7 Chinese ethnic origins were enumerated by quantitative polymerase chain reaction. Our data confirmed that the selected bacterial groups were common to all 7 surveyed ethnicities, but the amount of the individual bacterial groups varied to different degree. By principal component and canonical variate analyses of the 314 individuals or the 91 Han subjects, no distinct group clustering pattern was observed. Nevertheless, weak differences were noted between the Han and Zhuang from other ethnic minority groups, and between the Heilongjiang Hans from those of the other provinces. Thus, our results suggest that the ethnic origin may contribute to shaping the human gut microbiota.

Show MeSH