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Management of canine leishmaniosis in endemic SW European regions: a questionnaire-based multinational survey.

Bourdeau P, Saridomichelakis MN, Oliveira A, Oliva G, Kotnik T, Gálvez R, Foglia Manzillo V, Koutinas AF, Pereira da Fonseca I, Miró G - Parasit Vectors (2014)

Bottom Line: The data collected reflect similarities in the clinical manifestations reported although there was some variation in the concurrent diseases described, and wide variation in the clinical management of CanL among the countries examined in terms of dosing regimens, therapeutic agents and the criteria used to diagnose CanL.Most veterinarians properly informed dog owners about the preventive measures available and about the zoonotic implications of CanL.This survey describes the current situation in SW endemic countries in Europe regarding the clinical management of CanL.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda, Puerta de Hierro s/n, 28040 Madrid, Spain. gmiro@ucm.es.

ABSTRACT

Background: Canine leishmaniosis (CanL) caused by Leishmania infantum is a widespread endemic disease in SW Europe. This study was designed to determine how veterinarians clinically manage CanL in this region by analysing information collected in a questionnaire completed by local veterinarians working in clinics in France, Portugal, Greece, Spain, Italy and Slovenia.

Methods: Over the period 2004-2011, a questionnaire on CanL was sent to 12,546 small animal clinics located in the six countries surveyed. The questionnaire with 10 items comprising open and closed questions sought to obtain comparable data regarding the main clinical manifestations of CanL, the diagnostic methods used, the treatment regimens selected, recommended preventive measures and awareness of the important public health implications of CanL.

Results: The data collected reflect similarities in the clinical manifestations reported although there was some variation in the concurrent diseases described, and wide variation in the clinical management of CanL among the countries examined in terms of dosing regimens, therapeutic agents and the criteria used to diagnose CanL. Most veterinarians properly informed dog owners about the preventive measures available and about the zoonotic implications of CanL.

Conclusions: This survey describes the current situation in SW endemic countries in Europe regarding the clinical management of CanL. The data collected reveal a need to unify criteria from evidence-based medicine to determine and similarly apply the best diagnostic and treatment methods available for this disease in the different countries.

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Serology techniques used to diagnose CanL reported by veterinarians from Italy, Portugal, Spain, Greece and France.
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Figure 3: Serology techniques used to diagnose CanL reported by veterinarians from Italy, Portugal, Spain, Greece and France.

Mentions: Veterinarians reported microscopy examinations of lymph node aspirates as their first choice etiological diagnostic method (Figure 2). Cytology samples obtained from bone marrow and skin lesions showed good acceptance but were less used. Veterinarians who would never use an invasive technique for a bone marrow cytology were predominant in France (65.5%), Greece (69.6%) and Italy (59.9%) and those who never examined skin samples were predominant in Portugal (82.9%) and France (65%). As illustrated in Figure 2, an in-house etiological diagnosis consisting of microscopy observation of potentially infected tissue was not of routine use. Accordingly, a minority of veterinarians reported they always used a cytological method: lymph nodes (10%), bone marrow (7%) and skin lesions (6%). As complementary tests, microscopy observation of lymph node aspirates was the most used occasionally to frequently (62%). The quantitative serological method most used was IFAT (82.7%), followed by qualitative in-house rapid tests (78.2%) and ELISA (72.2%). For routine use, IFAT and qualitative rapid tests were described as always employed by 43% and 37% of veterinarians respectively (Figure 3). Qualitative rapid tests were most used for a routine diagnosis in Greece (58.3%), Portugal (63.5%), Spain (34.5%) and Italy (30.9%). Lastly, among the “Other techniques”, PCR was most reported (61%). Protein electrophoresis (14%) and immunohistochemistry (13%) were used more in routine practice than PCR (6%) or histopathology (1%) (Figure 4).


Management of canine leishmaniosis in endemic SW European regions: a questionnaire-based multinational survey.

Bourdeau P, Saridomichelakis MN, Oliveira A, Oliva G, Kotnik T, Gálvez R, Foglia Manzillo V, Koutinas AF, Pereira da Fonseca I, Miró G - Parasit Vectors (2014)

Serology techniques used to diagnose CanL reported by veterinarians from Italy, Portugal, Spain, Greece and France.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3974741&req=5

Figure 3: Serology techniques used to diagnose CanL reported by veterinarians from Italy, Portugal, Spain, Greece and France.
Mentions: Veterinarians reported microscopy examinations of lymph node aspirates as their first choice etiological diagnostic method (Figure 2). Cytology samples obtained from bone marrow and skin lesions showed good acceptance but were less used. Veterinarians who would never use an invasive technique for a bone marrow cytology were predominant in France (65.5%), Greece (69.6%) and Italy (59.9%) and those who never examined skin samples were predominant in Portugal (82.9%) and France (65%). As illustrated in Figure 2, an in-house etiological diagnosis consisting of microscopy observation of potentially infected tissue was not of routine use. Accordingly, a minority of veterinarians reported they always used a cytological method: lymph nodes (10%), bone marrow (7%) and skin lesions (6%). As complementary tests, microscopy observation of lymph node aspirates was the most used occasionally to frequently (62%). The quantitative serological method most used was IFAT (82.7%), followed by qualitative in-house rapid tests (78.2%) and ELISA (72.2%). For routine use, IFAT and qualitative rapid tests were described as always employed by 43% and 37% of veterinarians respectively (Figure 3). Qualitative rapid tests were most used for a routine diagnosis in Greece (58.3%), Portugal (63.5%), Spain (34.5%) and Italy (30.9%). Lastly, among the “Other techniques”, PCR was most reported (61%). Protein electrophoresis (14%) and immunohistochemistry (13%) were used more in routine practice than PCR (6%) or histopathology (1%) (Figure 4).

Bottom Line: The data collected reflect similarities in the clinical manifestations reported although there was some variation in the concurrent diseases described, and wide variation in the clinical management of CanL among the countries examined in terms of dosing regimens, therapeutic agents and the criteria used to diagnose CanL.Most veterinarians properly informed dog owners about the preventive measures available and about the zoonotic implications of CanL.This survey describes the current situation in SW endemic countries in Europe regarding the clinical management of CanL.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda, Puerta de Hierro s/n, 28040 Madrid, Spain. gmiro@ucm.es.

ABSTRACT

Background: Canine leishmaniosis (CanL) caused by Leishmania infantum is a widespread endemic disease in SW Europe. This study was designed to determine how veterinarians clinically manage CanL in this region by analysing information collected in a questionnaire completed by local veterinarians working in clinics in France, Portugal, Greece, Spain, Italy and Slovenia.

Methods: Over the period 2004-2011, a questionnaire on CanL was sent to 12,546 small animal clinics located in the six countries surveyed. The questionnaire with 10 items comprising open and closed questions sought to obtain comparable data regarding the main clinical manifestations of CanL, the diagnostic methods used, the treatment regimens selected, recommended preventive measures and awareness of the important public health implications of CanL.

Results: The data collected reflect similarities in the clinical manifestations reported although there was some variation in the concurrent diseases described, and wide variation in the clinical management of CanL among the countries examined in terms of dosing regimens, therapeutic agents and the criteria used to diagnose CanL. Most veterinarians properly informed dog owners about the preventive measures available and about the zoonotic implications of CanL.

Conclusions: This survey describes the current situation in SW endemic countries in Europe regarding the clinical management of CanL. The data collected reveal a need to unify criteria from evidence-based medicine to determine and similarly apply the best diagnostic and treatment methods available for this disease in the different countries.

Show MeSH
Related in: MedlinePlus