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Reduced expression of uroplakin 1A is associated with the poor prognosis of gastric adenocarcinoma patients.

Zheng Y, Wang DD, Wang W, Pan K, Huang CY, Li YF, Wang QJ, Yuan SQ, Jiang SS, Qiu HB, Chen YM, Zhang XF, Zhao BW, Mai C, Xia JC, Zhou ZW - PLoS ONE (2014)

Bottom Line: Additionally, the correlation analysis of clinicopathological factors demonstrated that reduced expression of UPK1A was significantly associated with histological grade (P = 0.022), node metastasis (P<0.001) and tumor node metastasis (TNM) stage (P = 0.008) (7th edition of the International Union Against Cancer (UICC)).Furthermore, Kaplan-Meier survival analysis revealed that the reduced expression of UPK1A was significantly associated with poor prognosis (P = 0.043).Thus, UPK1A could be a potential favorable biomarker associated with gastric cancer prognosis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oncology in South China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China; Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.

ABSTRACT

Background: The aim of this study was to investigate the expression and prognostic significance of Uroplakin1A (UPK1A) in gastric adenocarcinoma patients. Functional studies were also analyzed in vitro.

Methodology/principal findings: Real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical (IHC) staining methods were used to analyze the expression of UPK1A in primary gastric adenocarcinoma tissue samples. Compared with matched adjacent non-tumor, the expression of UPK1A in fresh surgical specimens was reduced, which was confirmed by RT-qPCR (P<0.01) and western blotting analysis (P<0.01). The paraffin specimens from a consecutive series of 445 gastric adenocarcinoma patients who underwent surgery between 2003 and 2006 were analyzed by IHC staining. The relationship between UPK1A expression, clinicopathological factors, and survival were evaluated. IHC staining analysis revealed that the reduced expression of UPK1A was observed in 224 cases (50.3%). Additionally, the correlation analysis of clinicopathological factors demonstrated that reduced expression of UPK1A was significantly associated with histological grade (P = 0.022), node metastasis (P<0.001) and tumor node metastasis (TNM) stage (P = 0.008) (7th edition of the International Union Against Cancer (UICC)). Furthermore, Kaplan-Meier survival analysis revealed that the reduced expression of UPK1A was significantly associated with poor prognosis (P = 0.043). Cox hazards model analysis indicated that UPK1A expression was an independent risk factor at the 0.1 level (P = 0.094). The function of UPK1A in cell cycle, migration, and invasion was investigated by overexpressing UPK1A in the MKN45 gastric cancer cell line. The elevated expression of UPK1A cells induced G1 phase arrest and significantly inhibited migration and invasion.

Conclusions/significance: The reduced expression of UPK1A might play a role in the progression of gastric cancer. Thus, UPK1A could be a potential favorable biomarker associated with gastric cancer prognosis.

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Related in: MedlinePlus

In the wound healing assay, representative images were photographed 0, 24, 48 and 72 hours after scratching.The result demonstrated that the high expression of UPK1A inhibited the cell motility.
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pone-0093073-g005: In the wound healing assay, representative images were photographed 0, 24, 48 and 72 hours after scratching.The result demonstrated that the high expression of UPK1A inhibited the cell motility.

Mentions: The wound healing and cell invasion assays were conducted to evaluate the effects of UPK1A expression on cell migration and invasion. In wound healing assays, the migration rate of MKN45-UPK1A cell was significantly reduced compared with MKN45-Vec cells (Figure 5). Consistent with the wound healing assay results, UPK1A also significantly inhibited cell invasion through a Matrigel-coated membrane in the invasion assay (pā€Š=ā€Š0.0407, Figure 6).


Reduced expression of uroplakin 1A is associated with the poor prognosis of gastric adenocarcinoma patients.

Zheng Y, Wang DD, Wang W, Pan K, Huang CY, Li YF, Wang QJ, Yuan SQ, Jiang SS, Qiu HB, Chen YM, Zhang XF, Zhao BW, Mai C, Xia JC, Zhou ZW - PLoS ONE (2014)

In the wound healing assay, representative images were photographed 0, 24, 48 and 72 hours after scratching.The result demonstrated that the high expression of UPK1A inhibited the cell motility.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974733&req=5

pone-0093073-g005: In the wound healing assay, representative images were photographed 0, 24, 48 and 72 hours after scratching.The result demonstrated that the high expression of UPK1A inhibited the cell motility.
Mentions: The wound healing and cell invasion assays were conducted to evaluate the effects of UPK1A expression on cell migration and invasion. In wound healing assays, the migration rate of MKN45-UPK1A cell was significantly reduced compared with MKN45-Vec cells (Figure 5). Consistent with the wound healing assay results, UPK1A also significantly inhibited cell invasion through a Matrigel-coated membrane in the invasion assay (pā€Š=ā€Š0.0407, Figure 6).

Bottom Line: Additionally, the correlation analysis of clinicopathological factors demonstrated that reduced expression of UPK1A was significantly associated with histological grade (P = 0.022), node metastasis (P<0.001) and tumor node metastasis (TNM) stage (P = 0.008) (7th edition of the International Union Against Cancer (UICC)).Furthermore, Kaplan-Meier survival analysis revealed that the reduced expression of UPK1A was significantly associated with poor prognosis (P = 0.043).Thus, UPK1A could be a potential favorable biomarker associated with gastric cancer prognosis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oncology in South China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China; Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.

ABSTRACT

Background: The aim of this study was to investigate the expression and prognostic significance of Uroplakin1A (UPK1A) in gastric adenocarcinoma patients. Functional studies were also analyzed in vitro.

Methodology/principal findings: Real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical (IHC) staining methods were used to analyze the expression of UPK1A in primary gastric adenocarcinoma tissue samples. Compared with matched adjacent non-tumor, the expression of UPK1A in fresh surgical specimens was reduced, which was confirmed by RT-qPCR (P<0.01) and western blotting analysis (P<0.01). The paraffin specimens from a consecutive series of 445 gastric adenocarcinoma patients who underwent surgery between 2003 and 2006 were analyzed by IHC staining. The relationship between UPK1A expression, clinicopathological factors, and survival were evaluated. IHC staining analysis revealed that the reduced expression of UPK1A was observed in 224 cases (50.3%). Additionally, the correlation analysis of clinicopathological factors demonstrated that reduced expression of UPK1A was significantly associated with histological grade (P = 0.022), node metastasis (P<0.001) and tumor node metastasis (TNM) stage (P = 0.008) (7th edition of the International Union Against Cancer (UICC)). Furthermore, Kaplan-Meier survival analysis revealed that the reduced expression of UPK1A was significantly associated with poor prognosis (P = 0.043). Cox hazards model analysis indicated that UPK1A expression was an independent risk factor at the 0.1 level (P = 0.094). The function of UPK1A in cell cycle, migration, and invasion was investigated by overexpressing UPK1A in the MKN45 gastric cancer cell line. The elevated expression of UPK1A cells induced G1 phase arrest and significantly inhibited migration and invasion.

Conclusions/significance: The reduced expression of UPK1A might play a role in the progression of gastric cancer. Thus, UPK1A could be a potential favorable biomarker associated with gastric cancer prognosis.

Show MeSH
Related in: MedlinePlus