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Boric acid inhibits germination and colonization of Saprolegnia spores in vitro and in vivo.

Ali SE, Thoen E, Evensen Ø, Skaar I - PLoS ONE (2014)

Bottom Line: The banning of malachite green increased the demand for finding effective alternative treatments to control the disease.Under in vitro conditions, boric acid was able to decrease Saprolegnia spore activity and mycelial growth in all tested concentrations above 0.2 g/L, while complete inhibition of germination and growth was observed at a concentration of 0.8 g/L.During the experiments no negative impact with regard to hatchability and viability was observed in either eggs or fry, which indicate safety of use at all tested concentrations.

View Article: PubMed Central - PubMed

Affiliation: Norwegian Veterinary Institute, Oslo, Norway.

ABSTRACT
Saprolegnia infections cause severe economic losses among freshwater fish and their eggs. The banning of malachite green increased the demand for finding effective alternative treatments to control the disease. In the present study, we investigated the ability of boric acid to control saprolegniosis in salmon eggs and yolk sac fry. Under in vitro conditions, boric acid was able to decrease Saprolegnia spore activity and mycelial growth in all tested concentrations above 0.2 g/L, while complete inhibition of germination and growth was observed at a concentration of 0.8 g/L. In in vivo experiments using Atlantic salmon eyed eggs, saprolegniosis was controlled by boric acid at concentrations ranging from 0.2-1.4 g/L during continuous exposure, and at 1.0-4.0 g/L during intermittent exposure. The same effect was observed on salmon yolk sac fry exposed continuously to 0.5 g/L boric acid during the natural outbreak of saprolegniosis. During the experiments no negative impact with regard to hatchability and viability was observed in either eggs or fry, which indicate safety of use at all tested concentrations. The high hatchability and survival rates recorded following the in vivo testing suggest that boric acid is a candidate for prophylaxis and control of saprolegniosis.

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Continuous exposure to boric acid.Mortality and hatching rates in boric acid treated groups and the non-treated control (0.0 g/L) at the end of the first experiment.
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pone-0091878-g008: Continuous exposure to boric acid.Mortality and hatching rates in boric acid treated groups and the non-treated control (0.0 g/L) at the end of the first experiment.

Mentions: At the end of the experiment and in groups treated at BA concentration >0.2 g/L the mycelium covering the eggs that were used as source of infection was scant, while many infected dead eggs were detected in the non-treated control group (Fig. 7). The percent of dead and hatched eggs were recorded after the termination of the experiment (Fig. 8). The bronopol treated group (positive control) showed 100% mortality after 4 h continuous exposure.


Boric acid inhibits germination and colonization of Saprolegnia spores in vitro and in vivo.

Ali SE, Thoen E, Evensen Ø, Skaar I - PLoS ONE (2014)

Continuous exposure to boric acid.Mortality and hatching rates in boric acid treated groups and the non-treated control (0.0 g/L) at the end of the first experiment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974685&req=5

pone-0091878-g008: Continuous exposure to boric acid.Mortality and hatching rates in boric acid treated groups and the non-treated control (0.0 g/L) at the end of the first experiment.
Mentions: At the end of the experiment and in groups treated at BA concentration >0.2 g/L the mycelium covering the eggs that were used as source of infection was scant, while many infected dead eggs were detected in the non-treated control group (Fig. 7). The percent of dead and hatched eggs were recorded after the termination of the experiment (Fig. 8). The bronopol treated group (positive control) showed 100% mortality after 4 h continuous exposure.

Bottom Line: The banning of malachite green increased the demand for finding effective alternative treatments to control the disease.Under in vitro conditions, boric acid was able to decrease Saprolegnia spore activity and mycelial growth in all tested concentrations above 0.2 g/L, while complete inhibition of germination and growth was observed at a concentration of 0.8 g/L.During the experiments no negative impact with regard to hatchability and viability was observed in either eggs or fry, which indicate safety of use at all tested concentrations.

View Article: PubMed Central - PubMed

Affiliation: Norwegian Veterinary Institute, Oslo, Norway.

ABSTRACT
Saprolegnia infections cause severe economic losses among freshwater fish and their eggs. The banning of malachite green increased the demand for finding effective alternative treatments to control the disease. In the present study, we investigated the ability of boric acid to control saprolegniosis in salmon eggs and yolk sac fry. Under in vitro conditions, boric acid was able to decrease Saprolegnia spore activity and mycelial growth in all tested concentrations above 0.2 g/L, while complete inhibition of germination and growth was observed at a concentration of 0.8 g/L. In in vivo experiments using Atlantic salmon eyed eggs, saprolegniosis was controlled by boric acid at concentrations ranging from 0.2-1.4 g/L during continuous exposure, and at 1.0-4.0 g/L during intermittent exposure. The same effect was observed on salmon yolk sac fry exposed continuously to 0.5 g/L boric acid during the natural outbreak of saprolegniosis. During the experiments no negative impact with regard to hatchability and viability was observed in either eggs or fry, which indicate safety of use at all tested concentrations. The high hatchability and survival rates recorded following the in vivo testing suggest that boric acid is a candidate for prophylaxis and control of saprolegniosis.

Show MeSH
Related in: MedlinePlus