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Gelam honey attenuated radiation-induced cell death in human diploid fibroblasts by promoting cell cycle progression and inhibiting apoptosis.

Tengku Ahmad TA, Jaafar F, Jubri Z, Abdul Rahim K, Rajab NF, Makpol S - BMC Complement Altern Med (2014)

Bottom Line: Pre-treatment with Gelam honey however caused down regulation of these genes in irradiated HDFs while no significant changes was observed on the expression of GADD45 and PAK genes.Gelam honey treatment however significantly decreased the expression of ATM, p73, and p16 proteins (p < 0.05) while the expression of cyclin D1 remained unchanged.Gelam honey acts a radioprotector against gamma-irradiation by attenuating radiation-induced cell death.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. suzanamakpol@yahoo.com.

ABSTRACT

Background: The interaction between ionizing radiation and substances in cells will induce the production of free radicals. These free radicals inflict damage to important biomolecules such as chromosomes, proteins and lipids which consequently trigger the expression of genes which are involved in protecting the cells or repair the oxidative damages. Honey has been known for its antioxidant properties and was used in medical and cosmetic products. Currently, research on honey is ongoing and diversifying. The aim of this study was to elucidate the role of Gelam honey as a radioprotector in human diploid fibroblast (HDFs) which were exposed to gamma-rays by determining the expression of genes and proteins involved in cell cycle regulation and cell death.

Methods: Six groups of HDFs were studied viz. untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/ml of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma-rays at the dose rate of 0.25 Gy/min.

Results: Our findings showed that, gamma-irradiation at 1 Gy up-regulated ATM, p53, p16ink4a and cyclin D1 genes and subsequently initiated cell cycle arrest at G0/G1 phase and induced apoptosis (p < 0.05). Pre-treatment with Gelam honey however caused down regulation of these genes in irradiated HDFs while no significant changes was observed on the expression of GADD45 and PAK genes. The expression of ATM and p16 proteins was increased in irradiated HDFs but the p53 gene was translated into p73 protein which was also increased in irradiated HDFs. Gelam honey treatment however significantly decreased the expression of ATM, p73, and p16 proteins (p < 0.05) while the expression of cyclin D1 remained unchanged. Analysis on cell cycle profile showed that cells progressed to S phase with less percentage of cells in G0/G1 phase with Gelam honey treatment while apoptosis was inhibited.

Conclusion: Gelam honey acts a radioprotector against gamma-irradiation by attenuating radiation-induced cell death.

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Related in: MedlinePlus

Relative expression value of p16ink4a gene in different treatment groups of HDFs. aDenotes p < 0.05 compared to untreated control, bp < 0.05 compared to irradiated HDFs. Data are expressed as mean ± SD (n = 6).
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Figure 5: Relative expression value of p16ink4a gene in different treatment groups of HDFs. aDenotes p < 0.05 compared to untreated control, bp < 0.05 compared to irradiated HDFs. Data are expressed as mean ± SD (n = 6).

Mentions: The p16ink4a gene was significantly up-regulated in irradiated HDFs as compared to untreated control (p < 0.05) (Figure 5). Down-regulation of p16ink4a gene was observed in HDFs treated with Gelam honey alone and irradiated HDFs pre-treated with Gelam honey as compared to irradiated HDFs (p < 0.05).


Gelam honey attenuated radiation-induced cell death in human diploid fibroblasts by promoting cell cycle progression and inhibiting apoptosis.

Tengku Ahmad TA, Jaafar F, Jubri Z, Abdul Rahim K, Rajab NF, Makpol S - BMC Complement Altern Med (2014)

Relative expression value of p16ink4a gene in different treatment groups of HDFs. aDenotes p < 0.05 compared to untreated control, bp < 0.05 compared to irradiated HDFs. Data are expressed as mean ± SD (n = 6).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3974451&req=5

Figure 5: Relative expression value of p16ink4a gene in different treatment groups of HDFs. aDenotes p < 0.05 compared to untreated control, bp < 0.05 compared to irradiated HDFs. Data are expressed as mean ± SD (n = 6).
Mentions: The p16ink4a gene was significantly up-regulated in irradiated HDFs as compared to untreated control (p < 0.05) (Figure 5). Down-regulation of p16ink4a gene was observed in HDFs treated with Gelam honey alone and irradiated HDFs pre-treated with Gelam honey as compared to irradiated HDFs (p < 0.05).

Bottom Line: Pre-treatment with Gelam honey however caused down regulation of these genes in irradiated HDFs while no significant changes was observed on the expression of GADD45 and PAK genes.Gelam honey treatment however significantly decreased the expression of ATM, p73, and p16 proteins (p < 0.05) while the expression of cyclin D1 remained unchanged.Gelam honey acts a radioprotector against gamma-irradiation by attenuating radiation-induced cell death.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. suzanamakpol@yahoo.com.

ABSTRACT

Background: The interaction between ionizing radiation and substances in cells will induce the production of free radicals. These free radicals inflict damage to important biomolecules such as chromosomes, proteins and lipids which consequently trigger the expression of genes which are involved in protecting the cells or repair the oxidative damages. Honey has been known for its antioxidant properties and was used in medical and cosmetic products. Currently, research on honey is ongoing and diversifying. The aim of this study was to elucidate the role of Gelam honey as a radioprotector in human diploid fibroblast (HDFs) which were exposed to gamma-rays by determining the expression of genes and proteins involved in cell cycle regulation and cell death.

Methods: Six groups of HDFs were studied viz. untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/ml of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma-rays at the dose rate of 0.25 Gy/min.

Results: Our findings showed that, gamma-irradiation at 1 Gy up-regulated ATM, p53, p16ink4a and cyclin D1 genes and subsequently initiated cell cycle arrest at G0/G1 phase and induced apoptosis (p < 0.05). Pre-treatment with Gelam honey however caused down regulation of these genes in irradiated HDFs while no significant changes was observed on the expression of GADD45 and PAK genes. The expression of ATM and p16 proteins was increased in irradiated HDFs but the p53 gene was translated into p73 protein which was also increased in irradiated HDFs. Gelam honey treatment however significantly decreased the expression of ATM, p73, and p16 proteins (p < 0.05) while the expression of cyclin D1 remained unchanged. Analysis on cell cycle profile showed that cells progressed to S phase with less percentage of cells in G0/G1 phase with Gelam honey treatment while apoptosis was inhibited.

Conclusion: Gelam honey acts a radioprotector against gamma-irradiation by attenuating radiation-induced cell death.

Show MeSH
Related in: MedlinePlus