Limits...
Inflammation in disseminated lesions: an analysis of CD4+, CD20+, CD68+, CD31+ and vW+ cells in non-ulcerated lesions of disseminated leishmaniasis.

Mendes DS, Dantas ML, Gomes JM, Santos WL, Silva AQ, Guimarães LH, Machado PR, Carvalho EM, Arruda S - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: Diffuse lymphoplasmacytic perivascular infiltrates were found in dermal skin.A significant linear correlation between CD4+ and CD20+ cells and the size of the inflamed area was also found.Thus, we demonstrate that vessels may have an important role in the clinical evolution of DL lesions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório Avançado de Saúde Pública, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, Brasil. dayanasantosmendes@gmail.com

ABSTRACT
Disseminated leishmaniasis (DL) differs from other clinical forms of the disease due to the presence of many non-ulcerated lesions (papules and nodules) in non-contiguous areas of the body. We describe the histopathology of DL non-ulcerated lesions and the presence of CD4-, CD20-, CD68-, CD31- and von Willebrand factor (vW)-positive cells in the inflamed area. We analysed eighteen biopsies from non-ulcerated lesions and quantified the inflamed areas and the expression of CD4, CD20, CD68, CD31 and vW using Image-Pro software (Media Cybernetics). Diffuse lymphoplasmacytic perivascular infiltrates were found in dermal skin. Inflammation was observed in 3-73% of the total biopsy area and showed a significant linear correlation with the number of vW+ vessels. The most common cells were CD68+ macrophages, CD20+ B-cells and CD4+ T-cells. A significant linear correlation between CD4+ and CD20+ cells and the size of the inflamed area was also found. Our findings show chronic inflammation in all DL non-ulcerated lesions predominantly formed by macrophages, plasmacytes and T and B-cells. As the inflamed area expanded, the number of granulomas and extent of the vascular framework increased. Thus, we demonstrate that vessels may have an important role in the clinical evolution of DL lesions.

Show MeSH

Related in: MedlinePlus

linear correlations of Pearson between CD4+T and inflamed area; B: linear correlations of Pearson between CD20+ B-cells and in flamed area; C: chronic perivascular inflammation stained with haematoxylin and eosin (200X).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3974311&req=5

f03: linear correlations of Pearson between CD4+T and inflamed area; B: linear correlations of Pearson between CD20+ B-cells and in flamed area; C: chronic perivascular inflammation stained with haematoxylin and eosin (200X).

Mentions: The inflammatory cells in non-ulcerated biopsies were quantified by number of cells per µm2. The following results were calculated: CD68+ macrophages, 103.4 ± 64.1 cells/µm2, CD20+ B-cells, 100.2 ± 72.2 cells/µm2 and CD4+ T-cells, 62.7 ± 32.8 cells/µm2 (Figs 1D, 2A). CD20+ B-cells and CD4+ T-cells were significantly associated with the extent of inflammation (Figs 2B, 3A, B). Other cells at the site of inflammation were not identified with the three cell markers used. It is likely that CD8+ T-cells, plasmacytes and undifferentiated cells could also be associated with inflammation in skin lesions after Leishmania dissemination.


Inflammation in disseminated lesions: an analysis of CD4+, CD20+, CD68+, CD31+ and vW+ cells in non-ulcerated lesions of disseminated leishmaniasis.

Mendes DS, Dantas ML, Gomes JM, Santos WL, Silva AQ, Guimarães LH, Machado PR, Carvalho EM, Arruda S - Mem. Inst. Oswaldo Cruz (2013)

linear correlations of Pearson between CD4+T and inflamed area; B: linear correlations of Pearson between CD20+ B-cells and in flamed area; C: chronic perivascular inflammation stained with haematoxylin and eosin (200X).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3974311&req=5

f03: linear correlations of Pearson between CD4+T and inflamed area; B: linear correlations of Pearson between CD20+ B-cells and in flamed area; C: chronic perivascular inflammation stained with haematoxylin and eosin (200X).
Mentions: The inflammatory cells in non-ulcerated biopsies were quantified by number of cells per µm2. The following results were calculated: CD68+ macrophages, 103.4 ± 64.1 cells/µm2, CD20+ B-cells, 100.2 ± 72.2 cells/µm2 and CD4+ T-cells, 62.7 ± 32.8 cells/µm2 (Figs 1D, 2A). CD20+ B-cells and CD4+ T-cells were significantly associated with the extent of inflammation (Figs 2B, 3A, B). Other cells at the site of inflammation were not identified with the three cell markers used. It is likely that CD8+ T-cells, plasmacytes and undifferentiated cells could also be associated with inflammation in skin lesions after Leishmania dissemination.

Bottom Line: Diffuse lymphoplasmacytic perivascular infiltrates were found in dermal skin.A significant linear correlation between CD4+ and CD20+ cells and the size of the inflamed area was also found.Thus, we demonstrate that vessels may have an important role in the clinical evolution of DL lesions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório Avançado de Saúde Pública, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, Brasil. dayanasantosmendes@gmail.com

ABSTRACT
Disseminated leishmaniasis (DL) differs from other clinical forms of the disease due to the presence of many non-ulcerated lesions (papules and nodules) in non-contiguous areas of the body. We describe the histopathology of DL non-ulcerated lesions and the presence of CD4-, CD20-, CD68-, CD31- and von Willebrand factor (vW)-positive cells in the inflamed area. We analysed eighteen biopsies from non-ulcerated lesions and quantified the inflamed areas and the expression of CD4, CD20, CD68, CD31 and vW using Image-Pro software (Media Cybernetics). Diffuse lymphoplasmacytic perivascular infiltrates were found in dermal skin. Inflammation was observed in 3-73% of the total biopsy area and showed a significant linear correlation with the number of vW+ vessels. The most common cells were CD68+ macrophages, CD20+ B-cells and CD4+ T-cells. A significant linear correlation between CD4+ and CD20+ cells and the size of the inflamed area was also found. Our findings show chronic inflammation in all DL non-ulcerated lesions predominantly formed by macrophages, plasmacytes and T and B-cells. As the inflamed area expanded, the number of granulomas and extent of the vascular framework increased. Thus, we demonstrate that vessels may have an important role in the clinical evolution of DL lesions.

Show MeSH
Related in: MedlinePlus