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Effects of Helicobacter pylori infection and long-term proton pump inhibitor use on enterochromaffin-like cells.

Bektaş M, Saraç N, Cetinkaya H, Törüner M, Erdemli E, Keskin O, Soykan I, Oktay EI, Korkut E, Ustün Y, Bahar K - Ann Gastroenterol (2012)

Bottom Line: In group B, moderate hyperplasia and vacuolization was seen in ECL cells.In group C, ECL cell hyperplasia was observed and vacuoles with greater amounts of granules in enlarged vesicles were found more intensely in cytoplasm.The use of PPI for a long period of time and presence of H. pylori infection are risk factors for ECL hyperplasia.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).

ABSTRACT

Background: Excessive release of gastrin leads to hypertrophy and hyperplasia of enterochromaffin-like cells (ECL) and prolonged stimulation of these cells causes functional impairment. The purpose of this study was to investigate the effect of Helicobacter pylori (H. pylori) infection and long-term proton pump inhibitors (PPI) use on ECL cells.

Methods: Fifteen patients who underwent endoscopy because of dyspeptic symptoms were enrolled in the present study. Biopsies were taken from corpus and antrum and existence of H. pylori was investigated with culture, cytology and CLOtest. The patients were divided into 3 groups. Group-A: H. pylori-negative, never treated previously with PPI; Group-B: H. pylori-positive, never treated previously with PPI; and group-C: H. pylori-negative and continuously treated with PPI for more than 6 months before the subject recruitment period. The features of ECL cell in oxyntic glands were examined with electron microscopy on biopsy specimens.

Results: ECL cells were completely normal in Group A. In group B, moderate hyperplasia and vacuolization was seen in ECL cells. In group C, ECL cell hyperplasia was observed and vacuoles with greater amounts of granules in enlarged vesicles were found more intensely in cytoplasm.

Conclusion: The use of PPI for a long period of time and presence of H. pylori infection are risk factors for ECL hyperplasia.

No MeSH data available.


Related in: MedlinePlus

Enterochromaffin-like (ECL) cell and cytoplasmic organelles. (a) normal ECL cell, (b) granules, (c) vacuoles, (d) lipofuscin
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Figure 1: Enterochromaffin-like (ECL) cell and cytoplasmic organelles. (a) normal ECL cell, (b) granules, (c) vacuoles, (d) lipofuscin

Mentions: Vesicles were membrane-enclosed, electron-lucent organelles, sometimes possessing a small, often eccentrically located dense core, which was less than 50% of the diameter of the vesicle. The lipofuscin bodies were identified by their high electron density and irregular shape. The ECL cells contained few lipofuscin bodies, numerous vesicles and vacuoles (Fig. 1). The cell numbers and cytoplasmic changes in all three groups were evaluated qualitatively. Fasting serum gastrin concentrations (normal range; 5.6-29.3 pg/mL) were measured in 5 mm3 blood samples drawn via venous route.


Effects of Helicobacter pylori infection and long-term proton pump inhibitor use on enterochromaffin-like cells.

Bektaş M, Saraç N, Cetinkaya H, Törüner M, Erdemli E, Keskin O, Soykan I, Oktay EI, Korkut E, Ustün Y, Bahar K - Ann Gastroenterol (2012)

Enterochromaffin-like (ECL) cell and cytoplasmic organelles. (a) normal ECL cell, (b) granules, (c) vacuoles, (d) lipofuscin
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3959398&req=5

Figure 1: Enterochromaffin-like (ECL) cell and cytoplasmic organelles. (a) normal ECL cell, (b) granules, (c) vacuoles, (d) lipofuscin
Mentions: Vesicles were membrane-enclosed, electron-lucent organelles, sometimes possessing a small, often eccentrically located dense core, which was less than 50% of the diameter of the vesicle. The lipofuscin bodies were identified by their high electron density and irregular shape. The ECL cells contained few lipofuscin bodies, numerous vesicles and vacuoles (Fig. 1). The cell numbers and cytoplasmic changes in all three groups were evaluated qualitatively. Fasting serum gastrin concentrations (normal range; 5.6-29.3 pg/mL) were measured in 5 mm3 blood samples drawn via venous route.

Bottom Line: In group B, moderate hyperplasia and vacuolization was seen in ECL cells.In group C, ECL cell hyperplasia was observed and vacuoles with greater amounts of granules in enlarged vesicles were found more intensely in cytoplasm.The use of PPI for a long period of time and presence of H. pylori infection are risk factors for ECL hyperplasia.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).

ABSTRACT

Background: Excessive release of gastrin leads to hypertrophy and hyperplasia of enterochromaffin-like cells (ECL) and prolonged stimulation of these cells causes functional impairment. The purpose of this study was to investigate the effect of Helicobacter pylori (H. pylori) infection and long-term proton pump inhibitors (PPI) use on ECL cells.

Methods: Fifteen patients who underwent endoscopy because of dyspeptic symptoms were enrolled in the present study. Biopsies were taken from corpus and antrum and existence of H. pylori was investigated with culture, cytology and CLOtest. The patients were divided into 3 groups. Group-A: H. pylori-negative, never treated previously with PPI; Group-B: H. pylori-positive, never treated previously with PPI; and group-C: H. pylori-negative and continuously treated with PPI for more than 6 months before the subject recruitment period. The features of ECL cell in oxyntic glands were examined with electron microscopy on biopsy specimens.

Results: ECL cells were completely normal in Group A. In group B, moderate hyperplasia and vacuolization was seen in ECL cells. In group C, ECL cell hyperplasia was observed and vacuoles with greater amounts of granules in enlarged vesicles were found more intensely in cytoplasm.

Conclusion: The use of PPI for a long period of time and presence of H. pylori infection are risk factors for ECL hyperplasia.

No MeSH data available.


Related in: MedlinePlus