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Simple and sure methodology for massive hepatectomy in the mouse.

Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Jermanus S, Nguyen JH - Ann Gastroenterol (2011)

Bottom Line: The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy.Learning curves and operative times were most optimal with the clip technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).

ABSTRACT

Background: Reliable models for massive hepatectomy in the mouse are required for experimental liver research.

Methods: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.

Results: According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.

Conclusion: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.

No MeSH data available.


Hepatic segments and transparent membranes. A. The murine liver is divided into six segments (RAS, RMS, RPS, LAS, LPS and OS). B. GB is detected between the RAS and LAS. The falciform ligament is located near the GB. The falciform and triangular ligaments are sharply cut to the surface level of the supra-hepatic inferior vena cava (arrow). The IHIVC should be skeletonized from the RPS (circle). C. The thin and transparent membranes that fix LPS to diaphragm (solid circle) and the OS (dotted circle) are sharply cut. D. The membrane that fixes the LAS and LPS to the hepatic hilum and gastroduodenal tract is sharply cut.RAS, right anterior segment; RMS, right middle segment; RPS, right posterior segment; LAS, left anterior segment; LPS, left posterior segment; OS, omental segment; GB, gallbladder; IHIVC, infrahepatic inferior vena cava.
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Figure 2: Hepatic segments and transparent membranes. A. The murine liver is divided into six segments (RAS, RMS, RPS, LAS, LPS and OS). B. GB is detected between the RAS and LAS. The falciform ligament is located near the GB. The falciform and triangular ligaments are sharply cut to the surface level of the supra-hepatic inferior vena cava (arrow). The IHIVC should be skeletonized from the RPS (circle). C. The thin and transparent membranes that fix LPS to diaphragm (solid circle) and the OS (dotted circle) are sharply cut. D. The membrane that fixes the LAS and LPS to the hepatic hilum and gastroduodenal tract is sharply cut.RAS, right anterior segment; RMS, right middle segment; RPS, right posterior segment; LAS, left anterior segment; LPS, left posterior segment; OS, omental segment; GB, gallbladder; IHIVC, infrahepatic inferior vena cava.

Mentions: The liver is divided into three lobes (right, left, and caudate lobes) arranged into six segments: right anterior segment (RAS), right middle segment (RMS), right posterior segment (RPS), left anterior segment (LAS), left posterior segment (LPS) and omental segment (OS) (Fig. 2A) (4-6). We measure the weight of each segment in a total of 100 mice, and segmental percentages were calculated as segmental weight (g)/whole liver weight (g) in each mouse. Of note, the murine gallbladder (GB) can be easily detected between the RAS and the LAS (Fig. 2B), while the rat does not have a GB.


Simple and sure methodology for massive hepatectomy in the mouse.

Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Jermanus S, Nguyen JH - Ann Gastroenterol (2011)

Hepatic segments and transparent membranes. A. The murine liver is divided into six segments (RAS, RMS, RPS, LAS, LPS and OS). B. GB is detected between the RAS and LAS. The falciform ligament is located near the GB. The falciform and triangular ligaments are sharply cut to the surface level of the supra-hepatic inferior vena cava (arrow). The IHIVC should be skeletonized from the RPS (circle). C. The thin and transparent membranes that fix LPS to diaphragm (solid circle) and the OS (dotted circle) are sharply cut. D. The membrane that fixes the LAS and LPS to the hepatic hilum and gastroduodenal tract is sharply cut.RAS, right anterior segment; RMS, right middle segment; RPS, right posterior segment; LAS, left anterior segment; LPS, left posterior segment; OS, omental segment; GB, gallbladder; IHIVC, infrahepatic inferior vena cava.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3959334&req=5

Figure 2: Hepatic segments and transparent membranes. A. The murine liver is divided into six segments (RAS, RMS, RPS, LAS, LPS and OS). B. GB is detected between the RAS and LAS. The falciform ligament is located near the GB. The falciform and triangular ligaments are sharply cut to the surface level of the supra-hepatic inferior vena cava (arrow). The IHIVC should be skeletonized from the RPS (circle). C. The thin and transparent membranes that fix LPS to diaphragm (solid circle) and the OS (dotted circle) are sharply cut. D. The membrane that fixes the LAS and LPS to the hepatic hilum and gastroduodenal tract is sharply cut.RAS, right anterior segment; RMS, right middle segment; RPS, right posterior segment; LAS, left anterior segment; LPS, left posterior segment; OS, omental segment; GB, gallbladder; IHIVC, infrahepatic inferior vena cava.
Mentions: The liver is divided into three lobes (right, left, and caudate lobes) arranged into six segments: right anterior segment (RAS), right middle segment (RMS), right posterior segment (RPS), left anterior segment (LAS), left posterior segment (LPS) and omental segment (OS) (Fig. 2A) (4-6). We measure the weight of each segment in a total of 100 mice, and segmental percentages were calculated as segmental weight (g)/whole liver weight (g) in each mouse. Of note, the murine gallbladder (GB) can be easily detected between the RAS and the LAS (Fig. 2B), while the rat does not have a GB.

Bottom Line: The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy.Learning curves and operative times were most optimal with the clip technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).

ABSTRACT

Background: Reliable models for massive hepatectomy in the mouse are required for experimental liver research.

Methods: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.

Results: According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.

Conclusion: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.

No MeSH data available.