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Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus

In vitro cytotoxicity of Nanomag-D-SPIO and SPIONs-C595 in OVCAR3 cell line by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. The cells were incubated with Nanomag-D-SPIO or SPIONs-C595 at equivalent Fe concentrations ranging from 0.2 to 2.4 mM for 2, 8, and 24 h
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Figure 4: In vitro cytotoxicity of Nanomag-D-SPIO and SPIONs-C595 in OVCAR3 cell line by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. The cells were incubated with Nanomag-D-SPIO or SPIONs-C595 at equivalent Fe concentrations ranging from 0.2 to 2.4 mM for 2, 8, and 24 h

Mentions: The in vitro cytotoxic effect of Nanomag-D-SPIO and the synthesized nanoprobe was assessed using the standard MTT assay, using ovarian cancer OVCAR3 cell line. As indicated in Figure 4, the results after different incubation times with different iron concentrations for cell line showed more than 80% cell viability in relation to the control. Moreover, because of their selective accumulation at cancer cells expressing the MUC1 cell receptor, only cells retaining the nanoparticles will be safe and nontoxic. These results are in good agreement with the results of previous published work,[18] which also showed a high affinity of SPIONs-C595 for OVCAR3 cell line and was a reason why this cell line is used for further investigation in this study.


Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

In vitro cytotoxicity of Nanomag-D-SPIO and SPIONs-C595 in OVCAR3 cell line by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. The cells were incubated with Nanomag-D-SPIO or SPIONs-C595 at equivalent Fe concentrations ranging from 0.2 to 2.4 mM for 2, 8, and 24 h
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3959000&req=5

Figure 4: In vitro cytotoxicity of Nanomag-D-SPIO and SPIONs-C595 in OVCAR3 cell line by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. The cells were incubated with Nanomag-D-SPIO or SPIONs-C595 at equivalent Fe concentrations ranging from 0.2 to 2.4 mM for 2, 8, and 24 h
Mentions: The in vitro cytotoxic effect of Nanomag-D-SPIO and the synthesized nanoprobe was assessed using the standard MTT assay, using ovarian cancer OVCAR3 cell line. As indicated in Figure 4, the results after different incubation times with different iron concentrations for cell line showed more than 80% cell viability in relation to the control. Moreover, because of their selective accumulation at cancer cells expressing the MUC1 cell receptor, only cells retaining the nanoparticles will be safe and nontoxic. These results are in good agreement with the results of previous published work,[18] which also showed a high affinity of SPIONs-C595 for OVCAR3 cell line and was a reason why this cell line is used for further investigation in this study.

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus