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Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus

TEM images for: (a) Nanomag-D-SPIO and (b) SPIONs-C595; antibody binding causes a significant reduction of particle agglomeration. The average particle size of particles estimated from TEM images was about 10-20 nm
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Figure 3: TEM images for: (a) Nanomag-D-SPIO and (b) SPIONs-C595; antibody binding causes a significant reduction of particle agglomeration. The average particle size of particles estimated from TEM images was about 10-20 nm

Mentions: The particle size distribution of SPIONs before and after mAb conjugation was obtained by PCS [Figure 2]. The hydrodynamic particle diameters were obtained to be 19.46 ± 0.80 and 27.22 ± 1.22 nm for Nanomag-D-SPIO and SPIONs-C595, respectively. Figure 3a and b show TEM images for the spherical shaped plain and mAb conjugated SPIONs, respectively. The average particle size calculated from TEM was 10-20 nm for two studied nanoparticle agents.


Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

TEM images for: (a) Nanomag-D-SPIO and (b) SPIONs-C595; antibody binding causes a significant reduction of particle agglomeration. The average particle size of particles estimated from TEM images was about 10-20 nm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3959000&req=5

Figure 3: TEM images for: (a) Nanomag-D-SPIO and (b) SPIONs-C595; antibody binding causes a significant reduction of particle agglomeration. The average particle size of particles estimated from TEM images was about 10-20 nm
Mentions: The particle size distribution of SPIONs before and after mAb conjugation was obtained by PCS [Figure 2]. The hydrodynamic particle diameters were obtained to be 19.46 ± 0.80 and 27.22 ± 1.22 nm for Nanomag-D-SPIO and SPIONs-C595, respectively. Figure 3a and b show TEM images for the spherical shaped plain and mAb conjugated SPIONs, respectively. The average particle size calculated from TEM was 10-20 nm for two studied nanoparticle agents.

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus