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Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus

The scheme of superparamagnetic iron oxide nanoparticle (SPIONs) conjugation to the C595 monoclonal antibody (mAb): (a) Functionalization of SPIO-CLD-NH2 with sulfo-SMCC and (b) conjugation of thiolated antibody to maleimide functionalized SPIONs
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Figure 1: The scheme of superparamagnetic iron oxide nanoparticle (SPIONs) conjugation to the C595 monoclonal antibody (mAb): (a) Functionalization of SPIO-CLD-NH2 with sulfo-SMCC and (b) conjugation of thiolated antibody to maleimide functionalized SPIONs

Mentions: The nanoprobe was synthesized as shown in [Figure 1], following a three-step process as described previously.[18] Briefly, in the first step, the sulfo-SMCC cross-linker was first added to nanomagĀ®-D-SPIO particles with CLD-NH2 surface to introduce maleimide groups. The next step was the modification of mAb primary amines with 2-iminothiolane to introduce sulfhydryl groups into the antibody. The sulfhydryl groups of antibody react with the maleimide groups at the surface of sulfo-SMCC activated SPIONs.


Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection.

Shahbazi-Gahrouei D, Abdolahi M - J Med Phys (2013)

The scheme of superparamagnetic iron oxide nanoparticle (SPIONs) conjugation to the C595 monoclonal antibody (mAb): (a) Functionalization of SPIO-CLD-NH2 with sulfo-SMCC and (b) conjugation of thiolated antibody to maleimide functionalized SPIONs
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3959000&req=5

Figure 1: The scheme of superparamagnetic iron oxide nanoparticle (SPIONs) conjugation to the C595 monoclonal antibody (mAb): (a) Functionalization of SPIO-CLD-NH2 with sulfo-SMCC and (b) conjugation of thiolated antibody to maleimide functionalized SPIONs
Mentions: The nanoprobe was synthesized as shown in [Figure 1], following a three-step process as described previously.[18] Briefly, in the first step, the sulfo-SMCC cross-linker was first added to nanomagĀ®-D-SPIO particles with CLD-NH2 surface to introduce maleimide groups. The next step was the modification of mAb primary amines with 2-iminothiolane to introduce sulfhydryl groups into the antibody. The sulfhydryl groups of antibody react with the maleimide groups at the surface of sulfo-SMCC activated SPIONs.

Bottom Line: The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell.Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer.In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted.

No MeSH data available.


Related in: MedlinePlus