Limits...
Study of non-organ-specific antibodies in children with genotype 4 chronic hepatitis C.

Hamed ME, Kamal Alanani NM, Sherief LM, Fouad MA, Elwahab LA, Raafat N - Saudi J Gastroenterol (2013 Nov-Dec)

Bottom Line: Epidemiologic and clinical features did not significantly differ between autoantibody-positive and -negative patients.Among biochemical features, significantly high levels of total bilirubin, albumin, immunoglobulins, alkaline phosphatase, and gamma-glutamyl transpeptidase were found in the antibody-positive group.More studies are warranted on larger pediatric population to validate these findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Hepatology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background/aim: Adult studies established a relationship between hepatitis C virus (HCV) infection and the presence of non-organ-specific antibodies (NOSAs). Most studies were carried out on genotypes 1 and 2. Only a few studies addressed that issue in pediatrics. No studies have been carried out on autoimmunity and genotype 4 in children. We aim to investigate NOSAs in 80 Egyptian children with chronic HCV infection along with studying the underlying genotype of HCV, and correlating autoimmunity with the epidemiological, clinical, biochemical, and virological features.

Materials and methods: HCV-RNA was assayed by the polymerase chain reaction and viral genotypes were determined. NOSAs were measured and liver biopsies were taken for histopathological examination.

Results: Genotype 4 was the only detected genotype in the included 80 patients. Anti-smooth muscle antibodies (ASMA) were the only detected antibodies in 32 (40%) patients, always with V specificity (vessels only) at titers ranging from 1:20 and 1:160. Anti-nuclear antibodies (ANA) and liver-kidney microsomal antibodies-1 (LKMA-1) were not detected in any of our patients. Epidemiologic and clinical features did not significantly differ between autoantibody-positive and -negative patients. Among biochemical features, significantly high levels of total bilirubin, albumin, immunoglobulins, alkaline phosphatase, and gamma-glutamyl transpeptidase were found in the antibody-positive group.

Conclusion: Genotype 4 HCV is the prevailing genotype in Egyptian children with chronic HCV infection. A consistent proportion of these children with chronic HCV infection circulate non-organ-specific autoantibodies. The prevalence of ASMA and the absence of ANA and LKMA-1 might be related to the unique situation in Egypt with unique prevalence of genotype 4. More studies are warranted on larger pediatric population to validate these findings.

Show MeSH

Related in: MedlinePlus

Comparison of mean values of bilirubin (total and direct) and proteins (total, albumin and globulin) in chronic HCV cases and controls
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3958974&req=5

Figure 1: Comparison of mean values of bilirubin (total and direct) and proteins (total, albumin and globulin) in chronic HCV cases and controls

Mentions: The details of correlations between autoantibodies and biochemistry are shown in Table 2 and Figures 1a–c. Patients with positive NOSAs had significantly higher total bilirubin, ALP, GGT, albumin, and IgG compared with NOSA-negative patients [Table 2 and Figures 1a–c].


Study of non-organ-specific antibodies in children with genotype 4 chronic hepatitis C.

Hamed ME, Kamal Alanani NM, Sherief LM, Fouad MA, Elwahab LA, Raafat N - Saudi J Gastroenterol (2013 Nov-Dec)

Comparison of mean values of bilirubin (total and direct) and proteins (total, albumin and globulin) in chronic HCV cases and controls
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958974&req=5

Figure 1: Comparison of mean values of bilirubin (total and direct) and proteins (total, albumin and globulin) in chronic HCV cases and controls
Mentions: The details of correlations between autoantibodies and biochemistry are shown in Table 2 and Figures 1a–c. Patients with positive NOSAs had significantly higher total bilirubin, ALP, GGT, albumin, and IgG compared with NOSA-negative patients [Table 2 and Figures 1a–c].

Bottom Line: Epidemiologic and clinical features did not significantly differ between autoantibody-positive and -negative patients.Among biochemical features, significantly high levels of total bilirubin, albumin, immunoglobulins, alkaline phosphatase, and gamma-glutamyl transpeptidase were found in the antibody-positive group.More studies are warranted on larger pediatric population to validate these findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Hepatology, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background/aim: Adult studies established a relationship between hepatitis C virus (HCV) infection and the presence of non-organ-specific antibodies (NOSAs). Most studies were carried out on genotypes 1 and 2. Only a few studies addressed that issue in pediatrics. No studies have been carried out on autoimmunity and genotype 4 in children. We aim to investigate NOSAs in 80 Egyptian children with chronic HCV infection along with studying the underlying genotype of HCV, and correlating autoimmunity with the epidemiological, clinical, biochemical, and virological features.

Materials and methods: HCV-RNA was assayed by the polymerase chain reaction and viral genotypes were determined. NOSAs were measured and liver biopsies were taken for histopathological examination.

Results: Genotype 4 was the only detected genotype in the included 80 patients. Anti-smooth muscle antibodies (ASMA) were the only detected antibodies in 32 (40%) patients, always with V specificity (vessels only) at titers ranging from 1:20 and 1:160. Anti-nuclear antibodies (ANA) and liver-kidney microsomal antibodies-1 (LKMA-1) were not detected in any of our patients. Epidemiologic and clinical features did not significantly differ between autoantibody-positive and -negative patients. Among biochemical features, significantly high levels of total bilirubin, albumin, immunoglobulins, alkaline phosphatase, and gamma-glutamyl transpeptidase were found in the antibody-positive group.

Conclusion: Genotype 4 HCV is the prevailing genotype in Egyptian children with chronic HCV infection. A consistent proportion of these children with chronic HCV infection circulate non-organ-specific autoantibodies. The prevalence of ASMA and the absence of ANA and LKMA-1 might be related to the unique situation in Egypt with unique prevalence of genotype 4. More studies are warranted on larger pediatric population to validate these findings.

Show MeSH
Related in: MedlinePlus