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Correlation between hepatitis B surface antigen titers and HBV DNA levels.

Alghamdi A, Aref N, El-Hazmi M, Al-Hamoudi W, Alswat K, Helmy A, Sanai FM, Abdo AA - Saudi J Gastroenterol (2013 Nov-Dec)

Bottom Line: A total of 106 treatment- naïve, HBeAg -ve HBV/D patients were included; 78 in the inactive carrier (IC) state and 28 in the active hepatitis (AH) stage.HBV DNA load and HBsAg titers were tested using TaqMan real-time polymerase chain reaction (PCR) and automated chemiluminescent microparticle immunoassay, respectively.The median (range) log10 HBsAg titer was significantly lower in the IC group compared with AH group, 3.09 (-1 to -4.4) versus 3.68 (-0.77 to 5.09) IU/mL, respectively; P < 0.001.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, College of Medicine; Liver Disease Research Center, King Saud University, Riyadh 11461, Saudi Arabia, .

ABSTRACT

Background/aim: To assess the correlation between serum HBsAg titers and hepatitis B virus (HBV) DNA levels in patients with hepatitis B envelop antigen-negative (HBeAg -ve) HBV genotype-D (HBV/D) infection.

Patients and methods: A total of 106 treatment- naïve, HBeAg -ve HBV/D patients were included; 78 in the inactive carrier (IC) state and 28 in the active hepatitis (AH) stage. HBV DNA load and HBsAg titers were tested using TaqMan real-time polymerase chain reaction (PCR) and automated chemiluminescent microparticle immunoassay, respectively.

Results: The median (range) log10 HBsAg titer was significantly lower in the IC group compared with AH group, 3.09 (-1 to -4.4) versus 3.68 (-0.77 to 5.09) IU/mL, respectively; P < 0.001. The suggested cutoff value of HBsAg titer to differentiate between the two groups was 3.79 log10 IU/mL. In addition, there was a significant positive correlation between HBsAg and HBV DNA levels in the whole cohort, AH, and IC groups (r = 0.6, P < 0.0001; r = 0.591, P = 0.001; and r = 0.243, P = 0.032, respectively).

Conclusion: Serum HBsAg titers may correlate with HBV DNA in treatment-naïve HBeAg -ve HBV/D patients, and supports the use of HBsAg levels in clinical practice as a predictor of serum HBV DNA levels.

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Related in: MedlinePlus

The area under the receiver–operating characteristic (ROC) curve for HBsAg levels in IC versus AH: AUROC = 0.705, 95% CI: 0.592-0.818, P < 0.05, with 67.9% sensitivity and 66.4% specificity
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Figure 1: The area under the receiver–operating characteristic (ROC) curve for HBsAg levels in IC versus AH: AUROC = 0.705, 95% CI: 0.592-0.818, P < 0.05, with 67.9% sensitivity and 66.4% specificity

Mentions: As shown in Table 1, the median serum HBV DNA level observed in the whole cohort (n = 106) was 2.79 log10 IU/mL, whereas the median serum HBsAg titer was 3.29 log10 IU/mL [Table 1]. As expected, the median log10 HBV DNA level was significantly lower in the IC group compared with the AH group patients; 2.57 (undetected–3.33 log10 IU/ml) versus 4.32 (3.36-8.30 log10 IU/mL), respectively; P < 0.0001. A similar pattern was observed for the log10 HBsAg titer whose median (range) was significantly lower in the IC group compared with the AH group patients; 3.09 (−1 to 4.4 log10 IU/mL) versus 3.68 (− 0.77 to 5.09 log10 IU/mL), respectively; P < 0.001. As shown in Figure 1, the AUROC curve of HBsAg titers was 0.705. The suggested cutoff value of HBsAg titer that differentiates between the two groups was found to be 3.46 log10 IU/mL with a sensitivity of 67.9%, a specificity of 66.4%, and a 95% CI of 0.592-0.818; P < 0.05


Correlation between hepatitis B surface antigen titers and HBV DNA levels.

Alghamdi A, Aref N, El-Hazmi M, Al-Hamoudi W, Alswat K, Helmy A, Sanai FM, Abdo AA - Saudi J Gastroenterol (2013 Nov-Dec)

The area under the receiver–operating characteristic (ROC) curve for HBsAg levels in IC versus AH: AUROC = 0.705, 95% CI: 0.592-0.818, P < 0.05, with 67.9% sensitivity and 66.4% specificity
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958972&req=5

Figure 1: The area under the receiver–operating characteristic (ROC) curve for HBsAg levels in IC versus AH: AUROC = 0.705, 95% CI: 0.592-0.818, P < 0.05, with 67.9% sensitivity and 66.4% specificity
Mentions: As shown in Table 1, the median serum HBV DNA level observed in the whole cohort (n = 106) was 2.79 log10 IU/mL, whereas the median serum HBsAg titer was 3.29 log10 IU/mL [Table 1]. As expected, the median log10 HBV DNA level was significantly lower in the IC group compared with the AH group patients; 2.57 (undetected–3.33 log10 IU/ml) versus 4.32 (3.36-8.30 log10 IU/mL), respectively; P < 0.0001. A similar pattern was observed for the log10 HBsAg titer whose median (range) was significantly lower in the IC group compared with the AH group patients; 3.09 (−1 to 4.4 log10 IU/mL) versus 3.68 (− 0.77 to 5.09 log10 IU/mL), respectively; P < 0.001. As shown in Figure 1, the AUROC curve of HBsAg titers was 0.705. The suggested cutoff value of HBsAg titer that differentiates between the two groups was found to be 3.46 log10 IU/mL with a sensitivity of 67.9%, a specificity of 66.4%, and a 95% CI of 0.592-0.818; P < 0.05

Bottom Line: A total of 106 treatment- naïve, HBeAg -ve HBV/D patients were included; 78 in the inactive carrier (IC) state and 28 in the active hepatitis (AH) stage.HBV DNA load and HBsAg titers were tested using TaqMan real-time polymerase chain reaction (PCR) and automated chemiluminescent microparticle immunoassay, respectively.The median (range) log10 HBsAg titer was significantly lower in the IC group compared with AH group, 3.09 (-1 to -4.4) versus 3.68 (-0.77 to 5.09) IU/mL, respectively; P < 0.001.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, College of Medicine; Liver Disease Research Center, King Saud University, Riyadh 11461, Saudi Arabia, .

ABSTRACT

Background/aim: To assess the correlation between serum HBsAg titers and hepatitis B virus (HBV) DNA levels in patients with hepatitis B envelop antigen-negative (HBeAg -ve) HBV genotype-D (HBV/D) infection.

Patients and methods: A total of 106 treatment- naïve, HBeAg -ve HBV/D patients were included; 78 in the inactive carrier (IC) state and 28 in the active hepatitis (AH) stage. HBV DNA load and HBsAg titers were tested using TaqMan real-time polymerase chain reaction (PCR) and automated chemiluminescent microparticle immunoassay, respectively.

Results: The median (range) log10 HBsAg titer was significantly lower in the IC group compared with AH group, 3.09 (-1 to -4.4) versus 3.68 (-0.77 to 5.09) IU/mL, respectively; P < 0.001. The suggested cutoff value of HBsAg titer to differentiate between the two groups was 3.79 log10 IU/mL. In addition, there was a significant positive correlation between HBsAg and HBV DNA levels in the whole cohort, AH, and IC groups (r = 0.6, P < 0.0001; r = 0.591, P = 0.001; and r = 0.243, P = 0.032, respectively).

Conclusion: Serum HBsAg titers may correlate with HBV DNA in treatment-naïve HBeAg -ve HBV/D patients, and supports the use of HBsAg levels in clinical practice as a predictor of serum HBV DNA levels.

Show MeSH
Related in: MedlinePlus