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β-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2.

Zhao Y, Qin G, Han R, Wang J, Zhang X, Liu D - Int J Mol Sci (2014)

Bottom Line: The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner.The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05).The reduced fluorescence of targets and change of cellular morphology were recorded.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. zhaoyuan4CL52@126.com.

ABSTRACT
Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001).

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Occludin relative mRNA expression of IPEC-J2 cells after 24 h incubation of β-conglycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b, c, d, e, f, g each represent two groups for different concentrations which are statistically different from each other.
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f6-ijms-15-01915: Occludin relative mRNA expression of IPEC-J2 cells after 24 h incubation of β-conglycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b, c, d, e, f, g each represent two groups for different concentrations which are statistically different from each other.

Mentions: Tight junction occludin or ZO-1 mRNA expression was assessed after 24 h exposure to β-conglycinin of different levels. The tight junction mRNA expression tended to linearly decline with increasing β-conglycinin (occludin: R2 = 0.941, p < 0.001; ZO-1, R2 = 0.956, p < 0.001) from 0.5–3 mg/mL (Figures 6 and 7). After 24 h incubation, the maximal reduction of occludin (versus control) and ZO-1 (versus control) was 57% and 59% for β-conglycinin.


β-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2.

Zhao Y, Qin G, Han R, Wang J, Zhang X, Liu D - Int J Mol Sci (2014)

Occludin relative mRNA expression of IPEC-J2 cells after 24 h incubation of β-conglycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b, c, d, e, f, g each represent two groups for different concentrations which are statistically different from each other.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958829&req=5

f6-ijms-15-01915: Occludin relative mRNA expression of IPEC-J2 cells after 24 h incubation of β-conglycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b, c, d, e, f, g each represent two groups for different concentrations which are statistically different from each other.
Mentions: Tight junction occludin or ZO-1 mRNA expression was assessed after 24 h exposure to β-conglycinin of different levels. The tight junction mRNA expression tended to linearly decline with increasing β-conglycinin (occludin: R2 = 0.941, p < 0.001; ZO-1, R2 = 0.956, p < 0.001) from 0.5–3 mg/mL (Figures 6 and 7). After 24 h incubation, the maximal reduction of occludin (versus control) and ZO-1 (versus control) was 57% and 59% for β-conglycinin.

Bottom Line: The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner.The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05).The reduced fluorescence of targets and change of cellular morphology were recorded.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. zhaoyuan4CL52@126.com.

ABSTRACT
Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001).

Show MeSH
Related in: MedlinePlus