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Inhibitory Potential of Five Traditionally Used Native Antidiabetic Medicinal Plants on α -Amylase, α -Glucosidase, Glucose Entrapment, and Amylolysis Kinetics In Vitro.

Picot CM, Subratty AH, Mahomoodally MF - Adv Pharmacol Sci (2014)

Bottom Line: Only methanolic extracts of EL, EO, and AM (7472.92 ± 5.99, 1745.58 ± 31.66, and 2222.96 ± 13.69  μ g/mL, resp.) were found to significantly (P < 0.05) inhibit α -amylase and were comparable to acarbose.EL, EO, AM, and SL extracts (5000  μ g/mL) were found to significantly (P < 0.05) inhibit α -glucosidase (between 87.41 ± 3.31 and 96.87 ± 1.37% inhibition).Extracts showed significant (P < 0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.).

View Article: PubMed Central - PubMed

Affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius.

ABSTRACT
Five traditionally used antidiabetic native medicinal plants of Mauritius, namely, Stillingia lineata (SL), Faujasiopsis flexuosa (FF), Erythroxylum laurifolium (EL), Elaeodendron orientale (EO), and Antidesma madagascariensis (AM), were studied for possible α -amylase and α -glucosidase inhibitory property, glucose entrapment, and amylolysis kinetics in vitro. Only methanolic extracts of EL, EO, and AM (7472.92 ± 5.99, 1745.58 ± 31.66, and 2222.96 ± 13.69  μ g/mL, resp.) were found to significantly (P < 0.05) inhibit α -amylase and were comparable to acarbose. EL, EO, AM, and SL extracts (5000  μ g/mL) were found to significantly (P < 0.05) inhibit α -glucosidase (between 87.41 ± 3.31 and 96.87 ± 1.37% inhibition). Enzyme kinetic studies showed an uncompetitive and mixed type of inhibition. Extracts showed significant (P < 0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.). Amylolysis kinetic studies showed that methanolic extracts were more potent inhibitors of α -amylase compared to aqueous extracts and possessed glucose entrapment properties. Our findings tend to provide justification for the hypoglycaemic action of these medicinal plants which has opened novel avenues for the development of new phytopharmaceuticals geared towards diabetes management.

No MeSH data available.


Related in: MedlinePlus

Percentage starch of reaction mixture and glucose concentration of dialysate in the presence of aqueous extracts. *Values [starch (%) concentration] significantly (P < 0.05) higher than negative control. †Values [glucose (mM) concentration] significantly (P < 0.05) lower than negative control.
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fig4: Percentage starch of reaction mixture and glucose concentration of dialysate in the presence of aqueous extracts. *Values [starch (%) concentration] significantly (P < 0.05) higher than negative control. †Values [glucose (mM) concentration] significantly (P < 0.05) lower than negative control.

Mentions: Figures 4 and 5 summarise the starch concentration (%) of the reaction mixture inside the dialysis bag and the glucose concentration (mM) of the surrounding solution after 4 h. Methanolic extracts were found to be potent inhibitors compared to their corresponding aqueous extracts. As observed by the α-amylase inhibition assay, methanolic EL, EO, and AM gave the best inhibitory activity since starch concentration was the highest in the presence of these extracts (Figure 5). Glucose dialysis was the least in the presence of methanolic SL extract.


Inhibitory Potential of Five Traditionally Used Native Antidiabetic Medicinal Plants on α -Amylase, α -Glucosidase, Glucose Entrapment, and Amylolysis Kinetics In Vitro.

Picot CM, Subratty AH, Mahomoodally MF - Adv Pharmacol Sci (2014)

Percentage starch of reaction mixture and glucose concentration of dialysate in the presence of aqueous extracts. *Values [starch (%) concentration] significantly (P < 0.05) higher than negative control. †Values [glucose (mM) concentration] significantly (P < 0.05) lower than negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958646&req=5

fig4: Percentage starch of reaction mixture and glucose concentration of dialysate in the presence of aqueous extracts. *Values [starch (%) concentration] significantly (P < 0.05) higher than negative control. †Values [glucose (mM) concentration] significantly (P < 0.05) lower than negative control.
Mentions: Figures 4 and 5 summarise the starch concentration (%) of the reaction mixture inside the dialysis bag and the glucose concentration (mM) of the surrounding solution after 4 h. Methanolic extracts were found to be potent inhibitors compared to their corresponding aqueous extracts. As observed by the α-amylase inhibition assay, methanolic EL, EO, and AM gave the best inhibitory activity since starch concentration was the highest in the presence of these extracts (Figure 5). Glucose dialysis was the least in the presence of methanolic SL extract.

Bottom Line: Only methanolic extracts of EL, EO, and AM (7472.92 ± 5.99, 1745.58 ± 31.66, and 2222.96 ± 13.69  μ g/mL, resp.) were found to significantly (P < 0.05) inhibit α -amylase and were comparable to acarbose.EL, EO, AM, and SL extracts (5000  μ g/mL) were found to significantly (P < 0.05) inhibit α -glucosidase (between 87.41 ± 3.31 and 96.87 ± 1.37% inhibition).Extracts showed significant (P < 0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.).

View Article: PubMed Central - PubMed

Affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius.

ABSTRACT
Five traditionally used antidiabetic native medicinal plants of Mauritius, namely, Stillingia lineata (SL), Faujasiopsis flexuosa (FF), Erythroxylum laurifolium (EL), Elaeodendron orientale (EO), and Antidesma madagascariensis (AM), were studied for possible α -amylase and α -glucosidase inhibitory property, glucose entrapment, and amylolysis kinetics in vitro. Only methanolic extracts of EL, EO, and AM (7472.92 ± 5.99, 1745.58 ± 31.66, and 2222.96 ± 13.69  μ g/mL, resp.) were found to significantly (P < 0.05) inhibit α -amylase and were comparable to acarbose. EL, EO, AM, and SL extracts (5000  μ g/mL) were found to significantly (P < 0.05) inhibit α -glucosidase (between 87.41 ± 3.31 and 96.87 ± 1.37% inhibition). Enzyme kinetic studies showed an uncompetitive and mixed type of inhibition. Extracts showed significant (P < 0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.). Amylolysis kinetic studies showed that methanolic extracts were more potent inhibitors of α -amylase compared to aqueous extracts and possessed glucose entrapment properties. Our findings tend to provide justification for the hypoglycaemic action of these medicinal plants which has opened novel avenues for the development of new phytopharmaceuticals geared towards diabetes management.

No MeSH data available.


Related in: MedlinePlus