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Apoptosis and inflammation associated gene expressions in monocrotaline-induced pulmonary hypertensive rats after bosentan treatment.

Hong YM, Kwon JH, Choi S, Kim KC - Korean Circ J (2014)

Bottom Line: Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.Bosentan may have potential for preventing apoptosis and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea. ; Ewha Womans University Global Top 5 Research Program, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT

Background and objectives: Vascular wall remodeling in pulmonary hypertension can be caused by an aberration in the normal balance between proliferation and apoptosis of endothelial cell in the pulmonary artery. The objective of this study was to evaluate the effect of bosentan on apoptosis in monocrotaline (MCT)-induced pulmonary hypertension.

Materials and methods: Sprague-Dawley rats were divided into three groups: control (C) group, M group (MCT 60 mg/kg) and B group (MCT 60 mg/kg plus bosentan 20 mg/day orally). Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.

Results: The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.

Conclusion: Bosentan may have potential for preventing apoptosis and inflammation.

No MeSH data available.


Related in: MedlinePlus

TUNEL assay results in lung tissues. There were no TUNEL positive cells (green) in the C group. TUNEL positivity was progressively increased by MCT treatment. Copious cell death was evident in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated than the M group. TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling, C: control group, M: monocrotaline group, B: bosentan group.
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Figure 7: TUNEL assay results in lung tissues. There were no TUNEL positive cells (green) in the C group. TUNEL positivity was progressively increased by MCT treatment. Copious cell death was evident in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated than the M group. TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling, C: control group, M: monocrotaline group, B: bosentan group.

Mentions: In western blot analyses, significant decreases in protein production were evident in the B group compared with the M group at week 4 in C-6 (p=0.041) (Fig. 5A), VEGF (p=0.045) (Fig. 5B), IL-6 (p=0.023) (Fig. 6A) and TNF-α (p=0.029) (Fig. 6B). The TUNEL assay revealed enhanced cell death was observed in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated compared with the M group (Fig. 7).


Apoptosis and inflammation associated gene expressions in monocrotaline-induced pulmonary hypertensive rats after bosentan treatment.

Hong YM, Kwon JH, Choi S, Kim KC - Korean Circ J (2014)

TUNEL assay results in lung tissues. There were no TUNEL positive cells (green) in the C group. TUNEL positivity was progressively increased by MCT treatment. Copious cell death was evident in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated than the M group. TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling, C: control group, M: monocrotaline group, B: bosentan group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958615&req=5

Figure 7: TUNEL assay results in lung tissues. There were no TUNEL positive cells (green) in the C group. TUNEL positivity was progressively increased by MCT treatment. Copious cell death was evident in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated than the M group. TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling, C: control group, M: monocrotaline group, B: bosentan group.
Mentions: In western blot analyses, significant decreases in protein production were evident in the B group compared with the M group at week 4 in C-6 (p=0.041) (Fig. 5A), VEGF (p=0.045) (Fig. 5B), IL-6 (p=0.023) (Fig. 6A) and TNF-α (p=0.029) (Fig. 6B). The TUNEL assay revealed enhanced cell death was observed in the M group compared with the C group. In contrast, apoptotic cell death in the B group was ameliorated compared with the M group (Fig. 7).

Bottom Line: Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.Bosentan may have potential for preventing apoptosis and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea. ; Ewha Womans University Global Top 5 Research Program, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT

Background and objectives: Vascular wall remodeling in pulmonary hypertension can be caused by an aberration in the normal balance between proliferation and apoptosis of endothelial cell in the pulmonary artery. The objective of this study was to evaluate the effect of bosentan on apoptosis in monocrotaline (MCT)-induced pulmonary hypertension.

Materials and methods: Sprague-Dawley rats were divided into three groups: control (C) group, M group (MCT 60 mg/kg) and B group (MCT 60 mg/kg plus bosentan 20 mg/day orally). Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.

Results: The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.

Conclusion: Bosentan may have potential for preventing apoptosis and inflammation.

No MeSH data available.


Related in: MedlinePlus