Limits...
Apoptosis and inflammation associated gene expressions in monocrotaline-induced pulmonary hypertensive rats after bosentan treatment.

Hong YM, Kwon JH, Choi S, Kim KC - Korean Circ J (2014)

Bottom Line: Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.Bosentan may have potential for preventing apoptosis and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea. ; Ewha Womans University Global Top 5 Research Program, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT

Background and objectives: Vascular wall remodeling in pulmonary hypertension can be caused by an aberration in the normal balance between proliferation and apoptosis of endothelial cell in the pulmonary artery. The objective of this study was to evaluate the effect of bosentan on apoptosis in monocrotaline (MCT)-induced pulmonary hypertension.

Materials and methods: Sprague-Dawley rats were divided into three groups: control (C) group, M group (MCT 60 mg/kg) and B group (MCT 60 mg/kg plus bosentan 20 mg/day orally). Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.

Results: The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.

Conclusion: Bosentan may have potential for preventing apoptosis and inflammation.

No MeSH data available.


Related in: MedlinePlus

Representative RT-PCR product of each gene. The RT-PCR products from the transcripts of Bcl-2, caspase-3, C-6, VEGF, IL-6, and TNF-α were 177 bp, 100 bp, 145 bp, 97 bp, 161 bp, and 113 bp. RT-PCR: reverse transscription-polymerase chain reaction, Bcl: B cell leukemia/lymphoma, C: complement component, VEGF: vascular endothelial growth factor, IL: interleukin, TNF: tumor necrosis factor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3958615&req=5

Figure 1: Representative RT-PCR product of each gene. The RT-PCR products from the transcripts of Bcl-2, caspase-3, C-6, VEGF, IL-6, and TNF-α were 177 bp, 100 bp, 145 bp, 97 bp, 161 bp, and 113 bp. RT-PCR: reverse transscription-polymerase chain reaction, Bcl: B cell leukemia/lymphoma, C: complement component, VEGF: vascular endothelial growth factor, IL: interleukin, TNF: tumor necrosis factor.

Mentions: The resulting first-strand of cDNA was normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. The normalized cDNA was used for the PCR procedure as a template. The specific primers for each gene are shown in Table 1. All primers were amplified using the same conditions. Thermal cycling conditions 50℃ for 2 minutes and 95℃ for 10 minutes followed by 40 cycles of 95℃ for 30 seconds and 60℃ for 30 seconds, and 72℃ for 30 seconds. To exclude the presence of unspecific products, a melting curve analysis of products was performed routinely after finishing amplification by a high-resolution data collection during an incremental temperature increase from 60℃ to 95℃ with a ramp rate of 0.21℃/second. Real-time PCR cycle numbers were converted to gene amounts (ng) on the basis of the equation. The real-time PCR analysis was performed on an Applied Biosystems Prism 7900 Sequence Detection System (Fig. 1).


Apoptosis and inflammation associated gene expressions in monocrotaline-induced pulmonary hypertensive rats after bosentan treatment.

Hong YM, Kwon JH, Choi S, Kim KC - Korean Circ J (2014)

Representative RT-PCR product of each gene. The RT-PCR products from the transcripts of Bcl-2, caspase-3, C-6, VEGF, IL-6, and TNF-α were 177 bp, 100 bp, 145 bp, 97 bp, 161 bp, and 113 bp. RT-PCR: reverse transscription-polymerase chain reaction, Bcl: B cell leukemia/lymphoma, C: complement component, VEGF: vascular endothelial growth factor, IL: interleukin, TNF: tumor necrosis factor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958615&req=5

Figure 1: Representative RT-PCR product of each gene. The RT-PCR products from the transcripts of Bcl-2, caspase-3, C-6, VEGF, IL-6, and TNF-α were 177 bp, 100 bp, 145 bp, 97 bp, 161 bp, and 113 bp. RT-PCR: reverse transscription-polymerase chain reaction, Bcl: B cell leukemia/lymphoma, C: complement component, VEGF: vascular endothelial growth factor, IL: interleukin, TNF: tumor necrosis factor.
Mentions: The resulting first-strand of cDNA was normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. The normalized cDNA was used for the PCR procedure as a template. The specific primers for each gene are shown in Table 1. All primers were amplified using the same conditions. Thermal cycling conditions 50℃ for 2 minutes and 95℃ for 10 minutes followed by 40 cycles of 95℃ for 30 seconds and 60℃ for 30 seconds, and 72℃ for 30 seconds. To exclude the presence of unspecific products, a melting curve analysis of products was performed routinely after finishing amplification by a high-resolution data collection during an incremental temperature increase from 60℃ to 95℃ with a ramp rate of 0.21℃/second. Real-time PCR cycle numbers were converted to gene amounts (ng) on the basis of the equation. The real-time PCR analysis was performed on an Applied Biosystems Prism 7900 Sequence Detection System (Fig. 1).

Bottom Line: Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.Bosentan may have potential for preventing apoptosis and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea. ; Ewha Womans University Global Top 5 Research Program, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT

Background and objectives: Vascular wall remodeling in pulmonary hypertension can be caused by an aberration in the normal balance between proliferation and apoptosis of endothelial cell in the pulmonary artery. The objective of this study was to evaluate the effect of bosentan on apoptosis in monocrotaline (MCT)-induced pulmonary hypertension.

Materials and methods: Sprague-Dawley rats were divided into three groups: control (C) group, M group (MCT 60 mg/kg) and B group (MCT 60 mg/kg plus bosentan 20 mg/day orally). Gene expressions of Bcl (B cell leukemia/lymphoma)-2, caspase-3, complement component (C)-6, vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were analyzed by real time polymerase chain reaction and western blot analysis.

Results: The messenger ribonucleic acid (mRNA) expressions of caspase-3 and VEGF were significantly increased in the M group compared with the C group, and significantly decreased in the B group compared with the M group in week 4. mRNA expression of IL-6 was significantly decreased in weeks 1, 2, and 4 in the B group compared with the M group. mRNA expression of TNF-α was significantly decreased on day 5 and in weeks 1 and 2 in the B group compared with the M group.

Conclusion: Bosentan may have potential for preventing apoptosis and inflammation.

No MeSH data available.


Related in: MedlinePlus