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Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

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Related in: MedlinePlus

The repeatability and the reproducibility of the response of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE. Conditions: scan rate 100 mV/s, pH 5.5 (a), pH 6.0 (b). The error bars indicate the standard error for three measurements with the same electrode.
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f9-sensors-14-03543: The repeatability and the reproducibility of the response of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE. Conditions: scan rate 100 mV/s, pH 5.5 (a), pH 6.0 (b). The error bars indicate the standard error for three measurements with the same electrode.

Mentions: The repeatability and the reproducibility of the two biosensors are illustrated in Figure 9. The error bars indicated the standard error for triplicate measurements with the same electrode. The relative standard deviation (RSD) of CNFs/Lac/Nafion/GCE and Cu/CNFs/Lac/Nafion/GCE were within 6.53% and 4.35%, respectively, indicating that Cu/CNFs/Lac/Nafion/GCE had better repeatability than CNFs/Lac/Nafion/GCE. The fabrication reproducibility of the two biosensors were calculated by cyclic voltammetric measurements on the same day, using five electrodes which were made independently under the optimum pH conditions previously established and in the presence of 3.92 × 10−4 M catechol. A RSD of CNFs/Lac/Nafion/GCE was 4.89% obtained, revealing an acceptable reproducibility in the construction of the electrode. Moreover, Cu/CNFs/Lac/Nafion/GCE with a RSD of 3.79% is better than CNF/Lac/Nafion/GCE in reproducibility. Also, the stability of the electrodes is considered. When the biosensor was not in use, it was stored in buffer at 4 °C.


Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

The repeatability and the reproducibility of the response of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE. Conditions: scan rate 100 mV/s, pH 5.5 (a), pH 6.0 (b). The error bars indicate the standard error for three measurements with the same electrode.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958252&req=5

f9-sensors-14-03543: The repeatability and the reproducibility of the response of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE. Conditions: scan rate 100 mV/s, pH 5.5 (a), pH 6.0 (b). The error bars indicate the standard error for three measurements with the same electrode.
Mentions: The repeatability and the reproducibility of the two biosensors are illustrated in Figure 9. The error bars indicated the standard error for triplicate measurements with the same electrode. The relative standard deviation (RSD) of CNFs/Lac/Nafion/GCE and Cu/CNFs/Lac/Nafion/GCE were within 6.53% and 4.35%, respectively, indicating that Cu/CNFs/Lac/Nafion/GCE had better repeatability than CNFs/Lac/Nafion/GCE. The fabrication reproducibility of the two biosensors were calculated by cyclic voltammetric measurements on the same day, using five electrodes which were made independently under the optimum pH conditions previously established and in the presence of 3.92 × 10−4 M catechol. A RSD of CNFs/Lac/Nafion/GCE was 4.89% obtained, revealing an acceptable reproducibility in the construction of the electrode. Moreover, Cu/CNFs/Lac/Nafion/GCE with a RSD of 3.79% is better than CNF/Lac/Nafion/GCE in reproducibility. Also, the stability of the electrodes is considered. When the biosensor was not in use, it was stored in buffer at 4 °C.

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

Show MeSH
Related in: MedlinePlus