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Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

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CVs of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE in 0.1 M, pH 4.0, acetate buffer solutions at scan rates from the inner to the outer: 50, 75, 100, 125, 150, 175, 200, 225 mV/s. Insets: calibration plots of anodic and cathodic peak currents vs. scan rates.
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f4-sensors-14-03543: CVs of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE in 0.1 M, pH 4.0, acetate buffer solutions at scan rates from the inner to the outer: 50, 75, 100, 125, 150, 175, 200, 225 mV/s. Insets: calibration plots of anodic and cathodic peak currents vs. scan rates.

Mentions: Figure 4 presents the cyclic voltammograms (CVs) of the CNFs/Lac/Nafion/GCE and Cu/CNFs/Lac/Nafion/GCE in pH 4.0 acetate buffer solutions with scan rates from 50 to 225 mV/s. From Figure 4a, it can be clearly seen that a pair of stable and well-defined quasi-reversible anodic and cathodic peaks were presented, which could be attributed to the direct electron transfer between laccase and GCE. The cathodic and anodic peak currents increased linearly with the increase of the scan rates in that range (insets in Figure 4). This revealed that the electron transfer between laccase and GCE was a quasi-reversible surface control process. Unlike CNFs/Lac/Nafion/GCE, Cu/CNFs/Lac/Nafion/GCE exhibited a sharp peak at the potential of 0 V (Figure 4b). It can be ascribed to the oxidation of copper. Compared with the ratio of Ipc to Ipa of 0.427 at CNFs/Lac/Nafion/GCE, the ratio of 0.757 at Cu/CNFs/Lac/Nafion/GCE was bigger, indicating a better reversibility at Cu/CNFs/Lac/Nafion/GCE.


Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

CVs of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE in 0.1 M, pH 4.0, acetate buffer solutions at scan rates from the inner to the outer: 50, 75, 100, 125, 150, 175, 200, 225 mV/s. Insets: calibration plots of anodic and cathodic peak currents vs. scan rates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958252&req=5

f4-sensors-14-03543: CVs of (a) CNFs/Lac/Nafion/GCE and (b) Cu/CNFs/Lac/Nafion/GCE in 0.1 M, pH 4.0, acetate buffer solutions at scan rates from the inner to the outer: 50, 75, 100, 125, 150, 175, 200, 225 mV/s. Insets: calibration plots of anodic and cathodic peak currents vs. scan rates.
Mentions: Figure 4 presents the cyclic voltammograms (CVs) of the CNFs/Lac/Nafion/GCE and Cu/CNFs/Lac/Nafion/GCE in pH 4.0 acetate buffer solutions with scan rates from 50 to 225 mV/s. From Figure 4a, it can be clearly seen that a pair of stable and well-defined quasi-reversible anodic and cathodic peaks were presented, which could be attributed to the direct electron transfer between laccase and GCE. The cathodic and anodic peak currents increased linearly with the increase of the scan rates in that range (insets in Figure 4). This revealed that the electron transfer between laccase and GCE was a quasi-reversible surface control process. Unlike CNFs/Lac/Nafion/GCE, Cu/CNFs/Lac/Nafion/GCE exhibited a sharp peak at the potential of 0 V (Figure 4b). It can be ascribed to the oxidation of copper. Compared with the ratio of Ipc to Ipa of 0.427 at CNFs/Lac/Nafion/GCE, the ratio of 0.757 at Cu/CNFs/Lac/Nafion/GCE was bigger, indicating a better reversibility at Cu/CNFs/Lac/Nafion/GCE.

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

Show MeSH
Related in: MedlinePlus