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Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

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XRD patterns of CNFs and Cu/CNFs.
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f2-sensors-14-03543: XRD patterns of CNFs and Cu/CNFs.

Mentions: Figure 2 presents the XRD patterns of CNFs and Cu/CNFs. It was observed that the pure CNFs exhibited two broad and weak diffraction peaks at 2θ = 24° and 43°, corresponding to the graphitic crystallite planes (002) and (100), respectively [32]. Unlike the pure CNFs, three diffraction peaks at 2θ = 44°, 51° and 74° were detected, which corresponded to the diffraction peaks of (111), (200) and (220) of Cu (JCPDS NO.04-0836). These diffraction peaks were narrow and sharp. The XRD pattern indicated that copper was well crystallized and cubic in structure. Based on the Scherrer equation, the crystallite size of copper nanoparticle in the composite fibers obtained at 900 °C was 20.8 nm. The XRD results confirmed the existence of carbon and copper in the composite nanofibers.


Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q - Sensors (Basel) (2014)

XRD patterns of CNFs and Cu/CNFs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3958252&req=5

f2-sensors-14-03543: XRD patterns of CNFs and Cu/CNFs.
Mentions: Figure 2 presents the XRD patterns of CNFs and Cu/CNFs. It was observed that the pure CNFs exhibited two broad and weak diffraction peaks at 2θ = 24° and 43°, corresponding to the graphitic crystallite planes (002) and (100), respectively [32]. Unlike the pure CNFs, three diffraction peaks at 2θ = 44°, 51° and 74° were detected, which corresponded to the diffraction peaks of (111), (200) and (220) of Cu (JCPDS NO.04-0836). These diffraction peaks were narrow and sharp. The XRD pattern indicated that copper was well crystallized and cubic in structure. Based on the Scherrer equation, the crystallite size of copper nanoparticle in the composite fibers obtained at 900 °C was 20.8 nm. The XRD results confirmed the existence of carbon and copper in the composite nanofibers.

Bottom Line: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE.Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. firgexiao@sina.cn.

ABSTRACT
The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10(-6) to 9.76 × 10(-3) M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.

Show MeSH
Related in: MedlinePlus