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Effect of alcohols on filamentation, growth, viability and biofilm development in Candida albicans.

Chauhan NM, Shinde RB, Karuppayil SM - Braz. J. Microbiol. (2014)

Bottom Line: It also inhibited normal biofilm development favoring yeast dominated biofilms.Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells.Since anaerobic conditions are not found to favor production of quorum sensing molecules like farnesol, the alcohols may play a role in morphogenetic regulation.

View Article: PubMed Central - PubMed

Affiliation: DST-FIST and UGC-SAP Sponsored School of Life Sciences, SRTM University, Nanded, M.S, India.

ABSTRACT
In this study we report the potential of alcohols as morphogenetic regulators in Candida albicans. All the alcohols tested influenced various modes of growth like planktonic as well as biofilm forms. Viability was affected at high concentrations. Among the alcohols, the response of C. albicans to amyl alcohol (pentanol) was noteworthy. Amyl alcohol at a concentration 0.5% which was not inhibitory to growth and viability specifically inhibited morphogenetic switching from yeast to hyphal forms. It also inhibited normal biofilm development favoring yeast dominated biofilms. Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells. Since anaerobic conditions are not found to favor production of quorum sensing molecules like farnesol, the alcohols may play a role in morphogenetic regulation.

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Effect of various alcohols on biofilm formation by Candida albicans. In vitro biofilm development in the presence of alcohols was quantified by using MTT assay. RPMI-1640 medium containing various concentrations of respective alcohols and one control without alcohol was incubated at 37 °C for 24 h.
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f2-bmj-44-4-1315: Effect of various alcohols on biofilm formation by Candida albicans. In vitro biofilm development in the presence of alcohols was quantified by using MTT assay. RPMI-1640 medium containing various concentrations of respective alcohols and one control without alcohol was incubated at 37 °C for 24 h.

Mentions: All the alcohols studied significantly (p = 0.0005) inhibited biofilm formation in a concentration dependent manner. Treatment with 4% of alcohols caused 50–60% reduction in metabolic activity in Candida biofilm (Figure 2). Addition of 2% of butanol, isobutanol, tertiary-butanol, propanol, and isopropanol after adhesion phase inhibited biofilm development and patches of few adhered yeast cells were observed, whereas similar effect was seen at 1% of pentanol (Figure 4). 1% of butanol, isobutanol, tertiary-butanol, propanol, and isopropanol inhibited filamentation in biofilms and favoured ‘yeast only’ biofilm. Pentanol caused considerable inhibition of biofilm at 0.5% concentration leading to yeast only biofilm formation.


Effect of alcohols on filamentation, growth, viability and biofilm development in Candida albicans.

Chauhan NM, Shinde RB, Karuppayil SM - Braz. J. Microbiol. (2014)

Effect of various alcohols on biofilm formation by Candida albicans. In vitro biofilm development in the presence of alcohols was quantified by using MTT assay. RPMI-1640 medium containing various concentrations of respective alcohols and one control without alcohol was incubated at 37 °C for 24 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958204&req=5

f2-bmj-44-4-1315: Effect of various alcohols on biofilm formation by Candida albicans. In vitro biofilm development in the presence of alcohols was quantified by using MTT assay. RPMI-1640 medium containing various concentrations of respective alcohols and one control without alcohol was incubated at 37 °C for 24 h.
Mentions: All the alcohols studied significantly (p = 0.0005) inhibited biofilm formation in a concentration dependent manner. Treatment with 4% of alcohols caused 50–60% reduction in metabolic activity in Candida biofilm (Figure 2). Addition of 2% of butanol, isobutanol, tertiary-butanol, propanol, and isopropanol after adhesion phase inhibited biofilm development and patches of few adhered yeast cells were observed, whereas similar effect was seen at 1% of pentanol (Figure 4). 1% of butanol, isobutanol, tertiary-butanol, propanol, and isopropanol inhibited filamentation in biofilms and favoured ‘yeast only’ biofilm. Pentanol caused considerable inhibition of biofilm at 0.5% concentration leading to yeast only biofilm formation.

Bottom Line: It also inhibited normal biofilm development favoring yeast dominated biofilms.Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells.Since anaerobic conditions are not found to favor production of quorum sensing molecules like farnesol, the alcohols may play a role in morphogenetic regulation.

View Article: PubMed Central - PubMed

Affiliation: DST-FIST and UGC-SAP Sponsored School of Life Sciences, SRTM University, Nanded, M.S, India.

ABSTRACT
In this study we report the potential of alcohols as morphogenetic regulators in Candida albicans. All the alcohols tested influenced various modes of growth like planktonic as well as biofilm forms. Viability was affected at high concentrations. Among the alcohols, the response of C. albicans to amyl alcohol (pentanol) was noteworthy. Amyl alcohol at a concentration 0.5% which was not inhibitory to growth and viability specifically inhibited morphogenetic switching from yeast to hyphal forms. It also inhibited normal biofilm development favoring yeast dominated biofilms. Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells. Since anaerobic conditions are not found to favor production of quorum sensing molecules like farnesol, the alcohols may play a role in morphogenetic regulation.

Show MeSH