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Halotolerance, ligninase production and herbicide degradation ability of basidiomycetes strains.

Arakaki RL, Monteiro DA, Boscolo M, Dasilva R, Gomes E - Braz. J. Microbiol. (2014)

Bottom Line: In this work, both the effect of salt and polyols on growth of the basidiomycetes strains, on their ability to produce ligninolytic enzyme and diuron degradation were evaluated.Seven out of ten tested strains had growth inhibited by salt while Dacryopinax elegans SXS323, Polyporus sp MCA128 and Datronia stereoides MCA167 fungi exhibited higher biomass production in medium containing 0.5 and 0.6 mol.L(-1) of NaCl, suggesting to be halotolerant.This fungus degraded diuron in medium control, in presence of NaCl as well as polyols, produced MnP, LiP and laccase.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Bioquímica Aplicada, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brazil.

ABSTRACT
Fungi have been recently recognized as organisms able to grow in presence of high salt concentration with halophilic and halotolerance properties and their ligninolytic enzyme complex have an unspecific action enabling their use to degradation of a number of xenobiotic compounds. In this work, both the effect of salt and polyols on growth of the basidiomycetes strains, on their ability to produce ligninolytic enzyme and diuron degradation were evaluated. Results showed that the presence of NaCl in the culture medium affected fungal specimens in different ways. Seven out of ten tested strains had growth inhibited by salt while Dacryopinax elegans SXS323, Polyporus sp MCA128 and Datronia stereoides MCA167 fungi exhibited higher biomass production in medium containing 0.5 and 0.6 mol.L(-1) of NaCl, suggesting to be halotolerant. Polyols such as glycerol and mannitol added into the culture media improved the biomass and ligninases production by D. elegans but the fungus did not reveal consumption of these polyols from media. This fungus degraded diuron in medium control, in presence of NaCl as well as polyols, produced MnP, LiP and laccase.

Show MeSH
Production of biomass and diuron degradation by Dacrypionax elegans SXS323. a, b = medium containing 0.03 M of glucose and 10 mg.L−1 of diuron; c, d = medium containing 0.03 M of glucose, 0.5 M of glycerol and 0.5 M of NaCl; e, f = medium containing 0.5 M of glucose and 0.5 M of glycerol. ■ = biomass without diuron, ● = biomass with diuron, Δ = diuron degradation; ◇ = remaining glycerol; ∇ = remaining glucose.
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Related In: Results  -  Collection


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f2-bmj-44-4-1207: Production of biomass and diuron degradation by Dacrypionax elegans SXS323. a, b = medium containing 0.03 M of glucose and 10 mg.L−1 of diuron; c, d = medium containing 0.03 M of glucose, 0.5 M of glycerol and 0.5 M of NaCl; e, f = medium containing 0.5 M of glucose and 0.5 M of glycerol. ■ = biomass without diuron, ● = biomass with diuron, Δ = diuron degradation; ◇ = remaining glycerol; ∇ = remaining glucose.

Mentions: Data from Figure 2a indicate that the growth of D. elegans was not affected by diuron, although the presence of both salt and diuron in the culture medium (Figure 2b) resulted in a lower biomass production suggesting therefore the increase in the sensitivity of fungi to herbicide in presence of salt. However when glycerol was added into the culture media, the biomass reached 7 g.L−1 (Figure 2e), twice the maximum production obtained in medium where these compounds were absent.


Halotolerance, ligninase production and herbicide degradation ability of basidiomycetes strains.

Arakaki RL, Monteiro DA, Boscolo M, Dasilva R, Gomes E - Braz. J. Microbiol. (2014)

Production of biomass and diuron degradation by Dacrypionax elegans SXS323. a, b = medium containing 0.03 M of glucose and 10 mg.L−1 of diuron; c, d = medium containing 0.03 M of glucose, 0.5 M of glycerol and 0.5 M of NaCl; e, f = medium containing 0.5 M of glucose and 0.5 M of glycerol. ■ = biomass without diuron, ● = biomass with diuron, Δ = diuron degradation; ◇ = remaining glycerol; ∇ = remaining glucose.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958189&req=5

f2-bmj-44-4-1207: Production of biomass and diuron degradation by Dacrypionax elegans SXS323. a, b = medium containing 0.03 M of glucose and 10 mg.L−1 of diuron; c, d = medium containing 0.03 M of glucose, 0.5 M of glycerol and 0.5 M of NaCl; e, f = medium containing 0.5 M of glucose and 0.5 M of glycerol. ■ = biomass without diuron, ● = biomass with diuron, Δ = diuron degradation; ◇ = remaining glycerol; ∇ = remaining glucose.
Mentions: Data from Figure 2a indicate that the growth of D. elegans was not affected by diuron, although the presence of both salt and diuron in the culture medium (Figure 2b) resulted in a lower biomass production suggesting therefore the increase in the sensitivity of fungi to herbicide in presence of salt. However when glycerol was added into the culture media, the biomass reached 7 g.L−1 (Figure 2e), twice the maximum production obtained in medium where these compounds were absent.

Bottom Line: In this work, both the effect of salt and polyols on growth of the basidiomycetes strains, on their ability to produce ligninolytic enzyme and diuron degradation were evaluated.Seven out of ten tested strains had growth inhibited by salt while Dacryopinax elegans SXS323, Polyporus sp MCA128 and Datronia stereoides MCA167 fungi exhibited higher biomass production in medium containing 0.5 and 0.6 mol.L(-1) of NaCl, suggesting to be halotolerant.This fungus degraded diuron in medium control, in presence of NaCl as well as polyols, produced MnP, LiP and laccase.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Bioquímica Aplicada, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brazil.

ABSTRACT
Fungi have been recently recognized as organisms able to grow in presence of high salt concentration with halophilic and halotolerance properties and their ligninolytic enzyme complex have an unspecific action enabling their use to degradation of a number of xenobiotic compounds. In this work, both the effect of salt and polyols on growth of the basidiomycetes strains, on their ability to produce ligninolytic enzyme and diuron degradation were evaluated. Results showed that the presence of NaCl in the culture medium affected fungal specimens in different ways. Seven out of ten tested strains had growth inhibited by salt while Dacryopinax elegans SXS323, Polyporus sp MCA128 and Datronia stereoides MCA167 fungi exhibited higher biomass production in medium containing 0.5 and 0.6 mol.L(-1) of NaCl, suggesting to be halotolerant. Polyols such as glycerol and mannitol added into the culture media improved the biomass and ligninases production by D. elegans but the fungus did not reveal consumption of these polyols from media. This fungus degraded diuron in medium control, in presence of NaCl as well as polyols, produced MnP, LiP and laccase.

Show MeSH