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Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production.

Oliveira AH, Ogrodowski CC, de Macedo AC, Santana MH, Gonçalves LR - Braz. J. Microbiol. (2014)

Bottom Line: In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium.Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations.The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil.

ABSTRACT
In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.

Show MeSH
Comparative performance of culture media for S. zooepidemicus groth: Crude cashew apple juice, CAJ, crude cashew apple juice, CAJ with 37 g/L or 60 g/L yeast extract, CAJ with 37 g/L Brain Heart Infusion, BHI and BHI medium 37 g/L.
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Related In: Results  -  Collection


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f1-bmj-44-4-1097: Comparative performance of culture media for S. zooepidemicus groth: Crude cashew apple juice, CAJ, crude cashew apple juice, CAJ with 37 g/L or 60 g/L yeast extract, CAJ with 37 g/L Brain Heart Infusion, BHI and BHI medium 37 g/L.

Mentions: Figure 1 shows the effect of culture medium on S. zooepidemicus growth during inoculum preparation. It can be observed that every medium induced colony growth and development, but low cell concentration (7.8 g/L) was obtained when pure CAJ was used. Enriching CAJ with 37 g/L BHI or using CAJY medium yielded similar biomass concentrations. The highest cell concentration was produced in CAJY as compared to the other media, including the control.


Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production.

Oliveira AH, Ogrodowski CC, de Macedo AC, Santana MH, Gonçalves LR - Braz. J. Microbiol. (2014)

Comparative performance of culture media for S. zooepidemicus groth: Crude cashew apple juice, CAJ, crude cashew apple juice, CAJ with 37 g/L or 60 g/L yeast extract, CAJ with 37 g/L Brain Heart Infusion, BHI and BHI medium 37 g/L.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958174&req=5

f1-bmj-44-4-1097: Comparative performance of culture media for S. zooepidemicus groth: Crude cashew apple juice, CAJ, crude cashew apple juice, CAJ with 37 g/L or 60 g/L yeast extract, CAJ with 37 g/L Brain Heart Infusion, BHI and BHI medium 37 g/L.
Mentions: Figure 1 shows the effect of culture medium on S. zooepidemicus growth during inoculum preparation. It can be observed that every medium induced colony growth and development, but low cell concentration (7.8 g/L) was obtained when pure CAJ was used. Enriching CAJ with 37 g/L BHI or using CAJY medium yielded similar biomass concentrations. The highest cell concentration was produced in CAJY as compared to the other media, including the control.

Bottom Line: In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium.Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations.The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil.

ABSTRACT
In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.

Show MeSH