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Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings.

Dayanandan A, Rani SH, Shanmugavel M, Gnanamani A, Rajakumar GS - Braz. J. Microbiol. (2014)

Bottom Line: The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C.Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility.The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.

ABSTRACT
The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.

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Effect of initial moisture content on lipase production.
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Related In: Results  -  Collection


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f1-bmj-44-4-1089: Effect of initial moisture content on lipase production.

Mentions: The initial moisture level of the fermentation media plays a vital role for the optimum production of enzymes (Pandey et al., 2000). In the present investigation, maximum lipase production was obtained in wheat bran containing 2.5% w/w gingily oil cake with 70% moisture content as growth medium (Figure 1). Increase or reduction of moisture level in this wheat bran-gingerly oil cake medium resulted in lower levels of lipase production. A similar trend was reported by Gutarra et al. (2005) with Penicillium simplicissimum in solid-state fermentation using babassu cake as the basal medium but Mahanta et al. (2008) reported initial moisture content at 50% of substrate as ideal for lipase production using Jatropha curcas seed cake. Therefore, it is evident that the enzyme production depends on the water holding capacity of the substrate and that it varies in various fermentation media.


Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings.

Dayanandan A, Rani SH, Shanmugavel M, Gnanamani A, Rajakumar GS - Braz. J. Microbiol. (2014)

Effect of initial moisture content on lipase production.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958173&req=5

f1-bmj-44-4-1089: Effect of initial moisture content on lipase production.
Mentions: The initial moisture level of the fermentation media plays a vital role for the optimum production of enzymes (Pandey et al., 2000). In the present investigation, maximum lipase production was obtained in wheat bran containing 2.5% w/w gingily oil cake with 70% moisture content as growth medium (Figure 1). Increase or reduction of moisture level in this wheat bran-gingerly oil cake medium resulted in lower levels of lipase production. A similar trend was reported by Gutarra et al. (2005) with Penicillium simplicissimum in solid-state fermentation using babassu cake as the basal medium but Mahanta et al. (2008) reported initial moisture content at 50% of substrate as ideal for lipase production using Jatropha curcas seed cake. Therefore, it is evident that the enzyme production depends on the water holding capacity of the substrate and that it varies in various fermentation media.

Bottom Line: The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C.Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility.The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.

ABSTRACT
The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.

Show MeSH