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Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064.

da Silva Vasconcelos E, de Lima VA, Goto LS, Cruz-Hernández IL, Hokka CO - Braz. J. Microbiol. (2014)

Bottom Line: In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064).The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores.The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.

ABSTRACT
Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064). The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores. Also, the strain MMS 150 presented a larger production of CA when cultivated in semi-synthetics media. Using other media, the wild type strain obtained a larger CA production. Besides, using the modifed complex media the MMS 150 strain showed changes in its lipolitic activity and a larger production of CA. The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.

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Related in: MedlinePlus

Comparison of the biomass (a), clavulanic acid (b) and lipolytic activity (c) between the mutant and wild type strains cultivated in the modified complex medium lacking glycerol.
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Related In: Results  -  Collection


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f4-bmj-44-4-1049: Comparison of the biomass (a), clavulanic acid (b) and lipolytic activity (c) between the mutant and wild type strains cultivated in the modified complex medium lacking glycerol.

Mentions: The wild type strain showed a simultaneous drop in the production of both clavulanic acid and lipase coincident with the depletion of glycerol. So, another cultivation study in complex medium deprived of glycerol was done. The results for the mutant strain show a greater lipase production when compared with the unmodified medium with a greater clavulanic acid production, leading to a suggestive correlation that lipase activity levels were elevated in the presence of oil as main source of carbon (Large et al., 1999). On the other hand, the wild type strain showed smaller values of biomass, lipolytic activity and clavulanic acid production when cultivated in the same complex medium lacking glycerol (Figure 4).


Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064.

da Silva Vasconcelos E, de Lima VA, Goto LS, Cruz-Hernández IL, Hokka CO - Braz. J. Microbiol. (2014)

Comparison of the biomass (a), clavulanic acid (b) and lipolytic activity (c) between the mutant and wild type strains cultivated in the modified complex medium lacking glycerol.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3958168&req=5

f4-bmj-44-4-1049: Comparison of the biomass (a), clavulanic acid (b) and lipolytic activity (c) between the mutant and wild type strains cultivated in the modified complex medium lacking glycerol.
Mentions: The wild type strain showed a simultaneous drop in the production of both clavulanic acid and lipase coincident with the depletion of glycerol. So, another cultivation study in complex medium deprived of glycerol was done. The results for the mutant strain show a greater lipase production when compared with the unmodified medium with a greater clavulanic acid production, leading to a suggestive correlation that lipase activity levels were elevated in the presence of oil as main source of carbon (Large et al., 1999). On the other hand, the wild type strain showed smaller values of biomass, lipolytic activity and clavulanic acid production when cultivated in the same complex medium lacking glycerol (Figure 4).

Bottom Line: In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064).The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores.The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.

ABSTRACT
Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064). The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores. Also, the strain MMS 150 presented a larger production of CA when cultivated in semi-synthetics media. Using other media, the wild type strain obtained a larger CA production. Besides, using the modifed complex media the MMS 150 strain showed changes in its lipolitic activity and a larger production of CA. The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.

Show MeSH
Related in: MedlinePlus