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Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells.

Shin Y, Lee Y - Toxicol Res (2013)

Bottom Line: α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood.In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells.These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Dongseo University, Busan, Korea.

ABSTRACT
α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with α-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. α-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of α-curcumene, which mediates cell death. These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

No MeSH data available.


Related in: MedlinePlus

Induction of cytochrome c release and caspase-3 activityby α-curcumene. SiHa cells were treated with each concentrationof by α-curcumene for 24 hr. (A) Mitochondrial cytochrome c was detected by anti-cytochrome c monoclonal antibody. Theaggregated cytochrome c, X-protein bends were used to normalizethe protein loading. (B) Caspase-3 activity was measuredby reading samples in fluorescence microplate reader. Data representsrelative activity of caspase-3 after normalization withprotein amounts. Data indicate mean values of three replicates,with bars indicating s.e.m. *p < 0.05 compared to control.
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Figure 004: Induction of cytochrome c release and caspase-3 activityby α-curcumene. SiHa cells were treated with each concentrationof by α-curcumene for 24 hr. (A) Mitochondrial cytochrome c was detected by anti-cytochrome c monoclonal antibody. Theaggregated cytochrome c, X-protein bends were used to normalizethe protein loading. (B) Caspase-3 activity was measuredby reading samples in fluorescence microplate reader. Data representsrelative activity of caspase-3 after normalization withprotein amounts. Data indicate mean values of three replicates,with bars indicating s.e.m. *p < 0.05 compared to control.

Mentions: Cytochrome c release and caspase-3 activation. Activation of caspases is regulated by the release of cytochrome c from mitochondria to the cytosol (22,23). The present study showed that cytochrome c release was markedly induced by treatment with α-curcumene for 24 hr (Fig. 4A). We confirmed these results using a caspase-3 activity assay. As shown in Fig. 4B, caspase-3 activities were increased 2.9 fold, 3.4 fold, by treatment with α-curcumene 300, 400 μM, respectively. These results suggest that α-curcumene induces apoptosis through the release of mitochondrial cytochrome c; a complex form with Apaf-1 subsequently activates caspase-3.


Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells.

Shin Y, Lee Y - Toxicol Res (2013)

Induction of cytochrome c release and caspase-3 activityby α-curcumene. SiHa cells were treated with each concentrationof by α-curcumene for 24 hr. (A) Mitochondrial cytochrome c was detected by anti-cytochrome c monoclonal antibody. Theaggregated cytochrome c, X-protein bends were used to normalizethe protein loading. (B) Caspase-3 activity was measuredby reading samples in fluorescence microplate reader. Data representsrelative activity of caspase-3 after normalization withprotein amounts. Data indicate mean values of three replicates,with bars indicating s.e.m. *p < 0.05 compared to control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3936178&req=5

Figure 004: Induction of cytochrome c release and caspase-3 activityby α-curcumene. SiHa cells were treated with each concentrationof by α-curcumene for 24 hr. (A) Mitochondrial cytochrome c was detected by anti-cytochrome c monoclonal antibody. Theaggregated cytochrome c, X-protein bends were used to normalizethe protein loading. (B) Caspase-3 activity was measuredby reading samples in fluorescence microplate reader. Data representsrelative activity of caspase-3 after normalization withprotein amounts. Data indicate mean values of three replicates,with bars indicating s.e.m. *p < 0.05 compared to control.
Mentions: Cytochrome c release and caspase-3 activation. Activation of caspases is regulated by the release of cytochrome c from mitochondria to the cytosol (22,23). The present study showed that cytochrome c release was markedly induced by treatment with α-curcumene for 24 hr (Fig. 4A). We confirmed these results using a caspase-3 activity assay. As shown in Fig. 4B, caspase-3 activities were increased 2.9 fold, 3.4 fold, by treatment with α-curcumene 300, 400 μM, respectively. These results suggest that α-curcumene induces apoptosis through the release of mitochondrial cytochrome c; a complex form with Apaf-1 subsequently activates caspase-3.

Bottom Line: α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood.In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells.These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Dongseo University, Busan, Korea.

ABSTRACT
α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with α-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. α-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of α-curcumene, which mediates cell death. These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

No MeSH data available.


Related in: MedlinePlus