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Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells.

Shin Y, Lee Y - Toxicol Res (2013)

Bottom Line: α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood.In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells.These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Dongseo University, Busan, Korea.

ABSTRACT
α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with α-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. α-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of α-curcumene, which mediates cell death. These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

No MeSH data available.


Related in: MedlinePlus

Decreased cell viability by α-curcumene in SiHa cells.After treatment of α-curcumene for 24 hr and 48 hr, the cell viabilitywas assessed by MTT staining. Results are expressed as thepercent change of the control condition in which the cells weregrown in the medium without drug. Data points represent themean values of four replicates with the bars indicating s.e.m.
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Figure 001: Decreased cell viability by α-curcumene in SiHa cells.After treatment of α-curcumene for 24 hr and 48 hr, the cell viabilitywas assessed by MTT staining. Results are expressed as thepercent change of the control condition in which the cells weregrown in the medium without drug. Data points represent themean values of four replicates with the bars indicating s.e.m.

Mentions: Inhibition of cell viability by α-curcumene. α-Curcumene showed a dose dependent inhibitory effect on SiHa cell proliferation. The cell viability was inhibited > 73% in SiHa cells exposed to 400 μM of α-curcumene, viability of SiHa cells was inhibited in a concentration dependent manner (Fig. 1). Inhibition percentage of 48 hr incubation is a little bit larger than that of 24 hr incubation at 300 μM of α-curcumene, and those were almost same at 400 μM of α-curcumene.


Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells.

Shin Y, Lee Y - Toxicol Res (2013)

Decreased cell viability by α-curcumene in SiHa cells.After treatment of α-curcumene for 24 hr and 48 hr, the cell viabilitywas assessed by MTT staining. Results are expressed as thepercent change of the control condition in which the cells weregrown in the medium without drug. Data points represent themean values of four replicates with the bars indicating s.e.m.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3936178&req=5

Figure 001: Decreased cell viability by α-curcumene in SiHa cells.After treatment of α-curcumene for 24 hr and 48 hr, the cell viabilitywas assessed by MTT staining. Results are expressed as thepercent change of the control condition in which the cells weregrown in the medium without drug. Data points represent themean values of four replicates with the bars indicating s.e.m.
Mentions: Inhibition of cell viability by α-curcumene. α-Curcumene showed a dose dependent inhibitory effect on SiHa cell proliferation. The cell viability was inhibited > 73% in SiHa cells exposed to 400 μM of α-curcumene, viability of SiHa cells was inhibited in a concentration dependent manner (Fig. 1). Inhibition percentage of 48 hr incubation is a little bit larger than that of 24 hr incubation at 300 μM of α-curcumene, and those were almost same at 400 μM of α-curcumene.

Bottom Line: α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood.In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells.These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Dongseo University, Busan, Korea.

ABSTRACT
α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with α-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. α-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of α-curcumene, which mediates cell death. These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.

No MeSH data available.


Related in: MedlinePlus