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Action Mechanism of Chamaecyparis obtusa Oil on Hair Growth.

Park YO, Kim SE, Kim YC - Toxicol Res (2013)

Bottom Line: At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group.Taking all the results together, on the basis of effects on ALP and γ-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase.Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health, Graduate School, Keimyung University, Daegu, Korea.

ABSTRACT
This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity that was significantly higher by 85% (p < 0.001) and 48% (p < 0.05) and an γ-GT activity that was significantly higher by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the saline (SA) treatment group. For insulin-like growth factor-1 (IGF-1) mRNA expression in the skin tissue, at week 4, the MXD and CO groups showed a significantly higher expression by 204% (p < 0.05) and 426% (p < 0.01) respectively, as compared to the SA group. At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group. Stem cell factor (SCF) expression in the MXD and CO groups was observed by immunohistochemistry as significant in a part of the bulge around the hair follicle and in a part of the basal layer of the epidermis. Taking all the results together, on the basis of effects on ALP and γ-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase. Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.

No MeSH data available.


Related in: MedlinePlus

Changes of γ-glutamyl transpeptidase activity in theskin of mice. SA: saline, MXD: 3% minoxidil, CO: 3% Chamaecyparisobtusa oil. Test compounds were topically applied to thebacks of C57BL/6 mice for 4 weeks. Values are mean ± SE of 5mice. *p< 0.05, **p< 0.01 compared to the SA group by ANOVAand Duncan’s multiple range tests.
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Figure 002: Changes of γ-glutamyl transpeptidase activity in theskin of mice. SA: saline, MXD: 3% minoxidil, CO: 3% Chamaecyparisobtusa oil. Test compounds were topically applied to thebacks of C57BL/6 mice for 4 weeks. Values are mean ± SE of 5mice. *p< 0.05, **p< 0.01 compared to the SA group by ANOVAand Duncan’s multiple range tests.

Mentions: Enzyme activities in the skin tissue. ALP activity in the skin tissue was shown in Fig. 1. At week 1, there were no significant differences among the 3 groups3, the MXD and CO groups showed high enzyme activities, as compared to the SA group, but there were no significant differences among the 3 groups4, the MXD and CO groups showed significantly higher activities by 85% (p < 0.001) and 48% (p < 0.05) respectively, as compared to the SA group. During the experimental period, the ALP activity in the MXD was increased gradually, whereas the CO group showed a decrease at week 4 (Fig. 1). γ-GT activity in the skin tissue was shown in Fig. 2. At weeks 1 and 3, there were no significant differences among the 3 groups. At week 4, the MXD and CO groups showed significantly higher activities by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the SA group. The γ-GT activities in the MXD and CO groups were increased gradually during the experimental period (Fig. 2).


Action Mechanism of Chamaecyparis obtusa Oil on Hair Growth.

Park YO, Kim SE, Kim YC - Toxicol Res (2013)

Changes of γ-glutamyl transpeptidase activity in theskin of mice. SA: saline, MXD: 3% minoxidil, CO: 3% Chamaecyparisobtusa oil. Test compounds were topically applied to thebacks of C57BL/6 mice for 4 weeks. Values are mean ± SE of 5mice. *p< 0.05, **p< 0.01 compared to the SA group by ANOVAand Duncan’s multiple range tests.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3936176&req=5

Figure 002: Changes of γ-glutamyl transpeptidase activity in theskin of mice. SA: saline, MXD: 3% minoxidil, CO: 3% Chamaecyparisobtusa oil. Test compounds were topically applied to thebacks of C57BL/6 mice for 4 weeks. Values are mean ± SE of 5mice. *p< 0.05, **p< 0.01 compared to the SA group by ANOVAand Duncan’s multiple range tests.
Mentions: Enzyme activities in the skin tissue. ALP activity in the skin tissue was shown in Fig. 1. At week 1, there were no significant differences among the 3 groups3, the MXD and CO groups showed high enzyme activities, as compared to the SA group, but there were no significant differences among the 3 groups4, the MXD and CO groups showed significantly higher activities by 85% (p < 0.001) and 48% (p < 0.05) respectively, as compared to the SA group. During the experimental period, the ALP activity in the MXD was increased gradually, whereas the CO group showed a decrease at week 4 (Fig. 1). γ-GT activity in the skin tissue was shown in Fig. 2. At weeks 1 and 3, there were no significant differences among the 3 groups. At week 4, the MXD and CO groups showed significantly higher activities by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the SA group. The γ-GT activities in the MXD and CO groups were increased gradually during the experimental period (Fig. 2).

Bottom Line: At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group.Taking all the results together, on the basis of effects on ALP and γ-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase.Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.

View Article: PubMed Central - PubMed

Affiliation: Department of Public Health, Graduate School, Keimyung University, Daegu, Korea.

ABSTRACT
This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity that was significantly higher by 85% (p < 0.001) and 48% (p < 0.05) and an γ-GT activity that was significantly higher by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the saline (SA) treatment group. For insulin-like growth factor-1 (IGF-1) mRNA expression in the skin tissue, at week 4, the MXD and CO groups showed a significantly higher expression by 204% (p < 0.05) and 426% (p < 0.01) respectively, as compared to the SA group. At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group. Stem cell factor (SCF) expression in the MXD and CO groups was observed by immunohistochemistry as significant in a part of the bulge around the hair follicle and in a part of the basal layer of the epidermis. Taking all the results together, on the basis of effects on ALP and γ-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase. Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.

No MeSH data available.


Related in: MedlinePlus