Limits...
Effects of fungi and eosinophils on mucin gene expression in rhinovirus-infected nasal epithelial cells.

Shin SH, Ye MK, Kim JK - Allergy Asthma Immunol Res (2013)

Bottom Line: Fungi, rhinoviruses (RVs), and eosinophils are associated with upper respiratory diseases.However, there was no synergistic increase in mucin gene expression, with the exception of MUC4 mRNA expression stimulated by 25 µg/mL Aspergillus.Mucin gene expression was inhibited by NF-κB inhibitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Catholic University of Daegu, School of Medicine, Daegu, Korea.

ABSTRACT

Purpose: Fungi, rhinoviruses (RVs), and eosinophils are associated with upper respiratory diseases. We evaluated the effects of fungal stimulation and eosinophil co-culture on the expression of mucin genes in RV-infected nasal polyp epithelial cells.

Methods: Nasal polyp epithelial cells were obtained from chronic rhinosinusitis patients. Cultured epithelial cells were stimulated with Alternaria and Aspergillus with or without RV-16 infection. The epithelial cells were co-cultured with eosinophils for 16 h. MUC4, MUC5AC, MUC5B, and MUC8 mRNA expressions in the epithelial cells were quantified using real-time RT-PCR. To determine the underlying mechanism, nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and mitogen-activated protein kinase (MAPK) inhibitors were used to inhibit mucin gene expression.

Results: Fungi and RV-16 induced mucin gene expression in nasal polyp epithelial cells. However, there was no synergistic increase in mucin gene expression, with the exception of MUC4 mRNA expression stimulated by 25 µg/mL Aspergillus. When RV-16-infected epithelial cells were stimulated with fungi and then co-cultured with eosinophils, MUC4, MUC5B, and MUC8 mRNA expressions increased. Mucin gene expression was inhibited by NF-κB inhibitors.

Conclusions: RV-16, airborne fungi, and eosinophils may exacerbate the inflammatory process in nasal mucosal diseases by enhancing mucin gene expression.

No MeSH data available.


Related in: MedlinePlus

Quantification of mucin gene expression in nasal polyp epithelial cells co-cultured with eosinophils and stimulated with Alternaria and Aspergillus with or without rhinovirus-16 (RV-16) infection for 16 h. Values are expressed as the means±SD of 6 independent experiments. Alt: Alternaria (µg/mL), Asp: Aspergillus (µg/mL), RV: rhinovirus-16-infected, EO: eosinophils, (-): unstimulated. *significantly higher than unstimulated cells, †significantly higher than RV-infected cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3936044&req=5

Figure 2: Quantification of mucin gene expression in nasal polyp epithelial cells co-cultured with eosinophils and stimulated with Alternaria and Aspergillus with or without rhinovirus-16 (RV-16) infection for 16 h. Values are expressed as the means±SD of 6 independent experiments. Alt: Alternaria (µg/mL), Asp: Aspergillus (µg/mL), RV: rhinovirus-16-infected, EO: eosinophils, (-): unstimulated. *significantly higher than unstimulated cells, †significantly higher than RV-infected cells.

Mentions: When NPECs were co-cultured with eosinophils with or without fungal stimulants, only MUC4 mRNA expression was significantly increased by Alternaria (50 µg/mL) and Aspergillus (25 µg/mL). When RV-16-infected NPECs were co-cultured with eosinophils, mucin gene expression was not affected. However, when these epithelial cells were pre-treated with fungi, MUC4 (Alternaria and Aspergillus), MUC5B and MUC8 (Alternaria), mRNA expressions were increased significantly. However, Alternaria-induced MUC4 mRNA expression was not synergistically increased with RV-16 infection of nasal polyp epithelial cells (Fig. 2).


Effects of fungi and eosinophils on mucin gene expression in rhinovirus-infected nasal epithelial cells.

Shin SH, Ye MK, Kim JK - Allergy Asthma Immunol Res (2013)

Quantification of mucin gene expression in nasal polyp epithelial cells co-cultured with eosinophils and stimulated with Alternaria and Aspergillus with or without rhinovirus-16 (RV-16) infection for 16 h. Values are expressed as the means±SD of 6 independent experiments. Alt: Alternaria (µg/mL), Asp: Aspergillus (µg/mL), RV: rhinovirus-16-infected, EO: eosinophils, (-): unstimulated. *significantly higher than unstimulated cells, †significantly higher than RV-infected cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3936044&req=5

Figure 2: Quantification of mucin gene expression in nasal polyp epithelial cells co-cultured with eosinophils and stimulated with Alternaria and Aspergillus with or without rhinovirus-16 (RV-16) infection for 16 h. Values are expressed as the means±SD of 6 independent experiments. Alt: Alternaria (µg/mL), Asp: Aspergillus (µg/mL), RV: rhinovirus-16-infected, EO: eosinophils, (-): unstimulated. *significantly higher than unstimulated cells, †significantly higher than RV-infected cells.
Mentions: When NPECs were co-cultured with eosinophils with or without fungal stimulants, only MUC4 mRNA expression was significantly increased by Alternaria (50 µg/mL) and Aspergillus (25 µg/mL). When RV-16-infected NPECs were co-cultured with eosinophils, mucin gene expression was not affected. However, when these epithelial cells were pre-treated with fungi, MUC4 (Alternaria and Aspergillus), MUC5B and MUC8 (Alternaria), mRNA expressions were increased significantly. However, Alternaria-induced MUC4 mRNA expression was not synergistically increased with RV-16 infection of nasal polyp epithelial cells (Fig. 2).

Bottom Line: Fungi, rhinoviruses (RVs), and eosinophils are associated with upper respiratory diseases.However, there was no synergistic increase in mucin gene expression, with the exception of MUC4 mRNA expression stimulated by 25 µg/mL Aspergillus.Mucin gene expression was inhibited by NF-κB inhibitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Catholic University of Daegu, School of Medicine, Daegu, Korea.

ABSTRACT

Purpose: Fungi, rhinoviruses (RVs), and eosinophils are associated with upper respiratory diseases. We evaluated the effects of fungal stimulation and eosinophil co-culture on the expression of mucin genes in RV-infected nasal polyp epithelial cells.

Methods: Nasal polyp epithelial cells were obtained from chronic rhinosinusitis patients. Cultured epithelial cells were stimulated with Alternaria and Aspergillus with or without RV-16 infection. The epithelial cells were co-cultured with eosinophils for 16 h. MUC4, MUC5AC, MUC5B, and MUC8 mRNA expressions in the epithelial cells were quantified using real-time RT-PCR. To determine the underlying mechanism, nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and mitogen-activated protein kinase (MAPK) inhibitors were used to inhibit mucin gene expression.

Results: Fungi and RV-16 induced mucin gene expression in nasal polyp epithelial cells. However, there was no synergistic increase in mucin gene expression, with the exception of MUC4 mRNA expression stimulated by 25 µg/mL Aspergillus. When RV-16-infected epithelial cells were stimulated with fungi and then co-cultured with eosinophils, MUC4, MUC5B, and MUC8 mRNA expressions increased. Mucin gene expression was inhibited by NF-κB inhibitors.

Conclusions: RV-16, airborne fungi, and eosinophils may exacerbate the inflammatory process in nasal mucosal diseases by enhancing mucin gene expression.

No MeSH data available.


Related in: MedlinePlus