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Vascular injury post stent implantation: different gene expression modulation in human umbilical vein endothelial cells (HUVECs) model.

Campolo J, Vozzi F, Penco S, Cozzi L, Caruso R, Domenici C, Ahluwalia A, Rial M, Marraccini P, Parodi O - PLoS ONE (2014)

Bottom Line: Different expressed genes, coming from Affymetrix results, were identified based on one-way ANOVA analysis with p values <0.01 and a fold changed >3 in modulus.Low shear stress was compared with physiological one in AS and PS conditions.Those genes belong to pathways specifically involved in the endothelial dysfunction.

View Article: PubMed Central - PubMed

Affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.

ABSTRACT
To explore whether stent procedure may influence transcriptional response of endothelium, we applied different physical (flow changes) and/or mechanical (stent application) stimuli to human endothelial cells in a laminar flow bioreactor (LFB) system. Gene expression analysis was then evaluated in each experimental condition. Human umbilical vein endothelial cells (HUVECs) were submitted to low and physiological (1 and 10 dyne/cm(2)) shear stress in absence (AS) or presence (PS) of stent positioning in a LFB system for 24 h. Different expressed genes, coming from Affymetrix results, were identified based on one-way ANOVA analysis with p values <0.01 and a fold changed >3 in modulus. Low shear stress was compared with physiological one in AS and PS conditions. Two major groups include 32 probes commonly expressed in both 1AS versus 10AS and 1PS versus 10PS comparison, and 115 probes consisting of 83 in addition to the previous 32, expressed only in 1PS versus 10PS comparison. Genes related to cytoskeleton, extracellular matrix, and cholesterol transport/metabolism are differently regulated in 1PS versus 10PS condition. Inflammatory and apoptotic mediators seems to be, instead, closely modulated by changes in flow (1 versus 10), independently of stent application. Low shear stress together with stent procedure are the experimental conditions that mainly modulate the highest number of genes in our human endothelial model. Those genes belong to pathways specifically involved in the endothelial dysfunction.

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Related in: MedlinePlus

Volcano blots of significance against the fold change of gene expression in different experimental conditions.A) Genes up and down regulated in 1AS vs 10AS condition. B) Genes up and down regulated in 1PS vs 10PS condition. The fold change was ≥3 in modulus with p values <0.01, determined individually for each gene. Horizontal continuous line indicates negative log of p = 0.01. Vertical dashed lines indicate the fold change cut-off.
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pone-0090213-g005: Volcano blots of significance against the fold change of gene expression in different experimental conditions.A) Genes up and down regulated in 1AS vs 10AS condition. B) Genes up and down regulated in 1PS vs 10PS condition. The fold change was ≥3 in modulus with p values <0.01, determined individually for each gene. Horizontal continuous line indicates negative log of p = 0.01. Vertical dashed lines indicate the fold change cut-off.

Mentions: One way ANOVA revealed 2761 genes of 40805 analyzed that are modulate in the experimental conditions. After filtering (FC > /3/), we observed that 32 ID probes were differently regulated by low shear stress compared to high flow (14 up- and 18 down-regulated) without stent positioning (Figure 5A). In addition, the stent presence differently regulated 115 ID probes (37 up- and 78 down-regulated) (Table 1 and Figure 5B). This last group of 115 ID contains also the same 32 probes present in low versus high flow comparison. Moreover, in physiological condition (F10) stent versus non stent presence showed only 3 probes down-expressed and no up-regulated genes were identified in our conditions.


Vascular injury post stent implantation: different gene expression modulation in human umbilical vein endothelial cells (HUVECs) model.

Campolo J, Vozzi F, Penco S, Cozzi L, Caruso R, Domenici C, Ahluwalia A, Rial M, Marraccini P, Parodi O - PLoS ONE (2014)

Volcano blots of significance against the fold change of gene expression in different experimental conditions.A) Genes up and down regulated in 1AS vs 10AS condition. B) Genes up and down regulated in 1PS vs 10PS condition. The fold change was ≥3 in modulus with p values <0.01, determined individually for each gene. Horizontal continuous line indicates negative log of p = 0.01. Vertical dashed lines indicate the fold change cut-off.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3935971&req=5

pone-0090213-g005: Volcano blots of significance against the fold change of gene expression in different experimental conditions.A) Genes up and down regulated in 1AS vs 10AS condition. B) Genes up and down regulated in 1PS vs 10PS condition. The fold change was ≥3 in modulus with p values <0.01, determined individually for each gene. Horizontal continuous line indicates negative log of p = 0.01. Vertical dashed lines indicate the fold change cut-off.
Mentions: One way ANOVA revealed 2761 genes of 40805 analyzed that are modulate in the experimental conditions. After filtering (FC > /3/), we observed that 32 ID probes were differently regulated by low shear stress compared to high flow (14 up- and 18 down-regulated) without stent positioning (Figure 5A). In addition, the stent presence differently regulated 115 ID probes (37 up- and 78 down-regulated) (Table 1 and Figure 5B). This last group of 115 ID contains also the same 32 probes present in low versus high flow comparison. Moreover, in physiological condition (F10) stent versus non stent presence showed only 3 probes down-expressed and no up-regulated genes were identified in our conditions.

Bottom Line: Different expressed genes, coming from Affymetrix results, were identified based on one-way ANOVA analysis with p values <0.01 and a fold changed >3 in modulus.Low shear stress was compared with physiological one in AS and PS conditions.Those genes belong to pathways specifically involved in the endothelial dysfunction.

View Article: PubMed Central - PubMed

Affiliation: CNR Institute of Clinical Physiology, Milan and Pisa, Italy.

ABSTRACT
To explore whether stent procedure may influence transcriptional response of endothelium, we applied different physical (flow changes) and/or mechanical (stent application) stimuli to human endothelial cells in a laminar flow bioreactor (LFB) system. Gene expression analysis was then evaluated in each experimental condition. Human umbilical vein endothelial cells (HUVECs) were submitted to low and physiological (1 and 10 dyne/cm(2)) shear stress in absence (AS) or presence (PS) of stent positioning in a LFB system for 24 h. Different expressed genes, coming from Affymetrix results, were identified based on one-way ANOVA analysis with p values <0.01 and a fold changed >3 in modulus. Low shear stress was compared with physiological one in AS and PS conditions. Two major groups include 32 probes commonly expressed in both 1AS versus 10AS and 1PS versus 10PS comparison, and 115 probes consisting of 83 in addition to the previous 32, expressed only in 1PS versus 10PS comparison. Genes related to cytoskeleton, extracellular matrix, and cholesterol transport/metabolism are differently regulated in 1PS versus 10PS condition. Inflammatory and apoptotic mediators seems to be, instead, closely modulated by changes in flow (1 versus 10), independently of stent application. Low shear stress together with stent procedure are the experimental conditions that mainly modulate the highest number of genes in our human endothelial model. Those genes belong to pathways specifically involved in the endothelial dysfunction.

Show MeSH
Related in: MedlinePlus