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Clonogenicity: holoclones and meroclones contain stem cells.

Beaver CM, Ahmed A, Masters JR - PLoS ONE (2014)

Bottom Line: When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones.These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively.We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers.

View Article: PubMed Central - PubMed

Affiliation: Prostate Cancer Research Centre, University College London, London, United Kingdom.

ABSTRACT
When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones. These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively. More recently, this approach has been extended to cancer cell lines. However, we observed that meroclones from the prostate cancer cell line DU145 produce holoclones, a paradoxical observation as meroclones are thought to be derived from transit-amplifying cells. The purpose of this study was to confirm this observation and determine if both holoclones and meroclones from cancer cell lines contain stem cells. We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers. This study demonstrates that the major difference between holoclones and meroclones derived from a cancer cell line is the proportion of stem cells within each colony, not the presence or absence of stem cells. These findings may reflect the properties of cancer as opposed to normal cells, perhaps indicating that the hierarchy of stem cells is more extensive in cancer.

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The proliferative fraction of DU145 colonies types determined by Ki67 staining.The percentage of Ki67 positive cells was determined by counting the number of green (FITC) cells as a proportion of blue DAPI positive nuclei. Representative colonies shown.
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pone-0089834-g003: The proliferative fraction of DU145 colonies types determined by Ki67 staining.The percentage of Ki67 positive cells was determined by counting the number of green (FITC) cells as a proportion of blue DAPI positive nuclei. Representative colonies shown.

Mentions: From three experiments, a total of 20 colonies of each type were fixed in paraformaldehyde and stained for Ki-67. The majority of the cells in all 3 types of colony were Ki-67 positive (Figure 3), with 86.6±3.6% positive in holoclones, 71.7±3.3% in meroclones and 58.9±7.5% in paraclones. Ki-67 expression was spread evenly throughout the colonies.


Clonogenicity: holoclones and meroclones contain stem cells.

Beaver CM, Ahmed A, Masters JR - PLoS ONE (2014)

The proliferative fraction of DU145 colonies types determined by Ki67 staining.The percentage of Ki67 positive cells was determined by counting the number of green (FITC) cells as a proportion of blue DAPI positive nuclei. Representative colonies shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3935944&req=5

pone-0089834-g003: The proliferative fraction of DU145 colonies types determined by Ki67 staining.The percentage of Ki67 positive cells was determined by counting the number of green (FITC) cells as a proportion of blue DAPI positive nuclei. Representative colonies shown.
Mentions: From three experiments, a total of 20 colonies of each type were fixed in paraformaldehyde and stained for Ki-67. The majority of the cells in all 3 types of colony were Ki-67 positive (Figure 3), with 86.6±3.6% positive in holoclones, 71.7±3.3% in meroclones and 58.9±7.5% in paraclones. Ki-67 expression was spread evenly throughout the colonies.

Bottom Line: When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones.These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively.We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers.

View Article: PubMed Central - PubMed

Affiliation: Prostate Cancer Research Centre, University College London, London, United Kingdom.

ABSTRACT
When primary cultures of normal cells are cloned, three types of colony grow, called holoclones, meroclones and paraclones. These colonies are believed to be derived from stem cells, transit-amplifying cells and differentiated cells respectively. More recently, this approach has been extended to cancer cell lines. However, we observed that meroclones from the prostate cancer cell line DU145 produce holoclones, a paradoxical observation as meroclones are thought to be derived from transit-amplifying cells. The purpose of this study was to confirm this observation and determine if both holoclones and meroclones from cancer cell lines contain stem cells. We demonstrated that both holoclones and meroclones can be serially passaged indefinitely, are highly proliferative, can self-renew to form spheres, are serially tumorigenic and express stem cell markers. This study demonstrates that the major difference between holoclones and meroclones derived from a cancer cell line is the proportion of stem cells within each colony, not the presence or absence of stem cells. These findings may reflect the properties of cancer as opposed to normal cells, perhaps indicating that the hierarchy of stem cells is more extensive in cancer.

Show MeSH
Related in: MedlinePlus