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WXG100 protein superfamily consists of three subfamilies and exhibits an α-helical C-terminal conserved residue pattern.

Poulsen C, Panjikar S, Holton SJ, Wilmanns M, Song YH - PLoS ONE (2014)

Bottom Line: The side chains of these conserved residues decorate the same side of the C-terminal α-helix and therefore form a distinct surface.Our results lead to a putatively extended T7SS secretion signal which combines two reported T7SS recognition characteristics: Firstly that the T7SS secretion signal is localized at the C-terminus of T7SS substrates and secondly that the conserved residues YxxxD/E are essential for T7SS activity.Furthermore, we propose that the specific α-helical surface formed by the conserved sequence pattern including YxxxD/E motif is a key component of T7SS-substrate recognition.

View Article: PubMed Central - PubMed

Affiliation: EMBL-Hamburg, Hamburg, Germany.

ABSTRACT
Members of the WXG100 protein superfamily form homo- or heterodimeric complexes. The most studied proteins among them are the secreted T-cell antigens CFP-10 (10 kDa culture filtrate protein, EsxB) and ESAT-6 (6 kDa early secreted antigen target, EsxA) from Mycobacterium tuberculosis. They are encoded on an operon within a gene cluster, named as ESX-1, that encodes for the Type VII secretion system (T7SS). WXG100 proteins are secreted in a full-length form and it is known that they adopt a four-helix bundle structure. In the current work we discuss the evolutionary relationship between the homo- and heterodimeric WXG100 proteins, the basis of the oligomeric state and the key structural features of the conserved sequence pattern of WXG100 proteins. We performed an iterative bioinformatics analysis of the WXG100 protein superfamily and correlated this with the atomic structures of the representative WXG100 proteins. We find, firstly, that the WXG100 protein superfamily consists of three subfamilies: CFP-10-, ESAT-6- and sagEsxA-like proteins (EsxA proteins similar to that of Streptococcus agalactiae). Secondly, that the heterodimeric complexes probably evolved from a homodimeric precursor. Thirdly, that the genes of hetero-dimeric WXG100 proteins are always encoded in bi-cistronic operons and finally, by combining the sequence alignments with the X-ray data we identify a conserved C-terminal sequence pattern. The side chains of these conserved residues decorate the same side of the C-terminal α-helix and therefore form a distinct surface. Our results lead to a putatively extended T7SS secretion signal which combines two reported T7SS recognition characteristics: Firstly that the T7SS secretion signal is localized at the C-terminus of T7SS substrates and secondly that the conserved residues YxxxD/E are essential for T7SS activity. Furthermore, we propose that the specific α-helical surface formed by the conserved sequence pattern including YxxxD/E motif is a key component of T7SS-substrate recognition.

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WXG100 proteins form dimeric complexes, studied using FRET.(A) Schematic diagram of the FRET experiments. Fluorescence donor, Alexa 488 (green), and fluorescence acceptor, Alexa 647 (red), are represented as stars. The Alexa fluorescence dye-conjugated proteins are indicated after their names along with the type of the Alexa dye, e.g. D-ESAT-6 instead of Alexa 488-ESAT-6. (B) The fluorescence spectra of the labelled proteins in those combinations, which were indicated in the schematic diagram A. Control contains only donor labelled protein (black). The donor/acceptor labelled ESAT-6 shows no FRET signal, also after heat de- and renaturation, indicating no homo-dimer formation (dark green). The donor labelled ESAT-6 and acceptor labelled CFP-10 gives a FRET signal, showing that CFP-10 and ESAT-6 spontaneously form a heterodimer (green). sagEsxA exhibits after initial mixing no FRET, but upon heat de- and renaturation there is reconstitution of FRET pairs (blue). For the FRET measurements the respective samples are mixed equimolar prior the measurements.
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pone-0089313-g005: WXG100 proteins form dimeric complexes, studied using FRET.(A) Schematic diagram of the FRET experiments. Fluorescence donor, Alexa 488 (green), and fluorescence acceptor, Alexa 647 (red), are represented as stars. The Alexa fluorescence dye-conjugated proteins are indicated after their names along with the type of the Alexa dye, e.g. D-ESAT-6 instead of Alexa 488-ESAT-6. (B) The fluorescence spectra of the labelled proteins in those combinations, which were indicated in the schematic diagram A. Control contains only donor labelled protein (black). The donor/acceptor labelled ESAT-6 shows no FRET signal, also after heat de- and renaturation, indicating no homo-dimer formation (dark green). The donor labelled ESAT-6 and acceptor labelled CFP-10 gives a FRET signal, showing that CFP-10 and ESAT-6 spontaneously form a heterodimer (green). sagEsxA exhibits after initial mixing no FRET, but upon heat de- and renaturation there is reconstitution of FRET pairs (blue). For the FRET measurements the respective samples are mixed equimolar prior the measurements.

Mentions: To investigate homo- versus heterodimer complex formation further, we carried out FRET (Förster Resonance Energy Transfer) measurements. The heterodimeric CFP-10/ESAT-6 complex (His6-CFP-10/ESAT-6) was decomposed into monomers under denaturing conditions and subsequently labelled chemically with amide active fluorescence dyes as described in Materials and Methods. The two monomers were conjugated separately with Alexa 488 (Donor-dye) or Alexa 647 (Acceptor-dye) to generate the four fluorescently modified monomers D-CFP-10, A-CFP-10, D-ESAT-6, and A-ESAT-6, respectively (Fig. 5A). There are theoretically four possible combinations of fluorescence samples as illustrated schematically (Fig. 5A). To detect the formation of dimers we recorded the static fluorescence intensity after mixing the donor labelled proteins with the acceptor labelled proteins. As a control we recorded a spectrum with a dimer where one monomer was labelled with the donor fluorophore and the second monomer was not labelled (Fig. 5B). A FRET signal could only be detected in those samples where donor-CFP-10 was mixed with acceptor-ESAT-6 or vice versa. These results demonstrate that CFP-10 and ESAT-6 exclusively form heterodimer and that heterodimer formation is a spontaneous process. The same experimental setup was employed to study the homodimeric protein sagEsxA. Interestingly, the sagEsxA FRET signal could only be detected after extensive heat treatment. This result shows that the homodimer is stable, and a FRET pair homodimer can only be reconstituted after heat dissociation (Fig. 5).


WXG100 protein superfamily consists of three subfamilies and exhibits an α-helical C-terminal conserved residue pattern.

Poulsen C, Panjikar S, Holton SJ, Wilmanns M, Song YH - PLoS ONE (2014)

WXG100 proteins form dimeric complexes, studied using FRET.(A) Schematic diagram of the FRET experiments. Fluorescence donor, Alexa 488 (green), and fluorescence acceptor, Alexa 647 (red), are represented as stars. The Alexa fluorescence dye-conjugated proteins are indicated after their names along with the type of the Alexa dye, e.g. D-ESAT-6 instead of Alexa 488-ESAT-6. (B) The fluorescence spectra of the labelled proteins in those combinations, which were indicated in the schematic diagram A. Control contains only donor labelled protein (black). The donor/acceptor labelled ESAT-6 shows no FRET signal, also after heat de- and renaturation, indicating no homo-dimer formation (dark green). The donor labelled ESAT-6 and acceptor labelled CFP-10 gives a FRET signal, showing that CFP-10 and ESAT-6 spontaneously form a heterodimer (green). sagEsxA exhibits after initial mixing no FRET, but upon heat de- and renaturation there is reconstitution of FRET pairs (blue). For the FRET measurements the respective samples are mixed equimolar prior the measurements.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3935865&req=5

pone-0089313-g005: WXG100 proteins form dimeric complexes, studied using FRET.(A) Schematic diagram of the FRET experiments. Fluorescence donor, Alexa 488 (green), and fluorescence acceptor, Alexa 647 (red), are represented as stars. The Alexa fluorescence dye-conjugated proteins are indicated after their names along with the type of the Alexa dye, e.g. D-ESAT-6 instead of Alexa 488-ESAT-6. (B) The fluorescence spectra of the labelled proteins in those combinations, which were indicated in the schematic diagram A. Control contains only donor labelled protein (black). The donor/acceptor labelled ESAT-6 shows no FRET signal, also after heat de- and renaturation, indicating no homo-dimer formation (dark green). The donor labelled ESAT-6 and acceptor labelled CFP-10 gives a FRET signal, showing that CFP-10 and ESAT-6 spontaneously form a heterodimer (green). sagEsxA exhibits after initial mixing no FRET, but upon heat de- and renaturation there is reconstitution of FRET pairs (blue). For the FRET measurements the respective samples are mixed equimolar prior the measurements.
Mentions: To investigate homo- versus heterodimer complex formation further, we carried out FRET (Förster Resonance Energy Transfer) measurements. The heterodimeric CFP-10/ESAT-6 complex (His6-CFP-10/ESAT-6) was decomposed into monomers under denaturing conditions and subsequently labelled chemically with amide active fluorescence dyes as described in Materials and Methods. The two monomers were conjugated separately with Alexa 488 (Donor-dye) or Alexa 647 (Acceptor-dye) to generate the four fluorescently modified monomers D-CFP-10, A-CFP-10, D-ESAT-6, and A-ESAT-6, respectively (Fig. 5A). There are theoretically four possible combinations of fluorescence samples as illustrated schematically (Fig. 5A). To detect the formation of dimers we recorded the static fluorescence intensity after mixing the donor labelled proteins with the acceptor labelled proteins. As a control we recorded a spectrum with a dimer where one monomer was labelled with the donor fluorophore and the second monomer was not labelled (Fig. 5B). A FRET signal could only be detected in those samples where donor-CFP-10 was mixed with acceptor-ESAT-6 or vice versa. These results demonstrate that CFP-10 and ESAT-6 exclusively form heterodimer and that heterodimer formation is a spontaneous process. The same experimental setup was employed to study the homodimeric protein sagEsxA. Interestingly, the sagEsxA FRET signal could only be detected after extensive heat treatment. This result shows that the homodimer is stable, and a FRET pair homodimer can only be reconstituted after heat dissociation (Fig. 5).

Bottom Line: The side chains of these conserved residues decorate the same side of the C-terminal α-helix and therefore form a distinct surface.Our results lead to a putatively extended T7SS secretion signal which combines two reported T7SS recognition characteristics: Firstly that the T7SS secretion signal is localized at the C-terminus of T7SS substrates and secondly that the conserved residues YxxxD/E are essential for T7SS activity.Furthermore, we propose that the specific α-helical surface formed by the conserved sequence pattern including YxxxD/E motif is a key component of T7SS-substrate recognition.

View Article: PubMed Central - PubMed

Affiliation: EMBL-Hamburg, Hamburg, Germany.

ABSTRACT
Members of the WXG100 protein superfamily form homo- or heterodimeric complexes. The most studied proteins among them are the secreted T-cell antigens CFP-10 (10 kDa culture filtrate protein, EsxB) and ESAT-6 (6 kDa early secreted antigen target, EsxA) from Mycobacterium tuberculosis. They are encoded on an operon within a gene cluster, named as ESX-1, that encodes for the Type VII secretion system (T7SS). WXG100 proteins are secreted in a full-length form and it is known that they adopt a four-helix bundle structure. In the current work we discuss the evolutionary relationship between the homo- and heterodimeric WXG100 proteins, the basis of the oligomeric state and the key structural features of the conserved sequence pattern of WXG100 proteins. We performed an iterative bioinformatics analysis of the WXG100 protein superfamily and correlated this with the atomic structures of the representative WXG100 proteins. We find, firstly, that the WXG100 protein superfamily consists of three subfamilies: CFP-10-, ESAT-6- and sagEsxA-like proteins (EsxA proteins similar to that of Streptococcus agalactiae). Secondly, that the heterodimeric complexes probably evolved from a homodimeric precursor. Thirdly, that the genes of hetero-dimeric WXG100 proteins are always encoded in bi-cistronic operons and finally, by combining the sequence alignments with the X-ray data we identify a conserved C-terminal sequence pattern. The side chains of these conserved residues decorate the same side of the C-terminal α-helix and therefore form a distinct surface. Our results lead to a putatively extended T7SS secretion signal which combines two reported T7SS recognition characteristics: Firstly that the T7SS secretion signal is localized at the C-terminus of T7SS substrates and secondly that the conserved residues YxxxD/E are essential for T7SS activity. Furthermore, we propose that the specific α-helical surface formed by the conserved sequence pattern including YxxxD/E motif is a key component of T7SS-substrate recognition.

Show MeSH
Related in: MedlinePlus