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Remineralization of artificial caries in primary teeth by grape seed extract: an in vitro study.

Mirkarimi M, Eskandarion S, Bargrizan M, Delazar A, Kharazifard MJ - J Dent Res Dent Clin Dent Prospects (2013)

Bottom Line: Results.Grape seed extract significantly increased the micro-hardness of the lesions (P=0.03).Conclusion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Assistant Professor, Department of Pediatric dentistry, Children and Adolescent Health Research Center, Zahedan University of Medical Sciences, Za-hedan, Iran.

ABSTRACT
Background and aims. Promoting remineralization is the ultimate goal of clinical prevention of caries lesion. The present in vitro study aimed to investigate the effect of grape seed extract (GSE) on artificial enamel caries in primary human teeth. Materials and methods. Seventeen human sound primary incisors were sectioned mesiodistally. The tooth slices were placed in a demineralizing solution for 96 hours at 37ºC and 50% relative humidity to create lesions. The demineralized fragments of each tooth were randomly divided into two case (immersed in GSE solution in phosphate buffer for 8 days) and control (immersed in distilled water) groups. The samples were subsequently evaluated using a scanning electron microscope and a micro-hardness tester. Data were analyzed using independent t-test. Results. The mean ± SD micro-hardness values for the case and control groups were 358.6±83.42 and 296.51± 69.41, respectively. Grape seed extract significantly increased the micro-hardness of the lesions (P=0.03). The morphology of GSE treated enamel was clearly different from that in the control group, and there were deposits of scaffolding insoluble complexes on the enamel surface. Conclusion. GSE enhanced the remineralization process of artificial enamel lesions of primary teeth, and thus, might be considered an effective natural agent in non-invasive dentistry.

No MeSH data available.


Related in: MedlinePlus

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Mentions: Figure 1shows the SEM photomicrograph of sound enamel (×1000 magnification), revealing an orderly smooth appearance. There are also some spherical particles on the surface.Figure 2shows the photomicrograph of demineralized enamel (×1000 magnification) before treatment; the enamel surface is rough and disorganized with significant porosities. In Figure 3Ashowing the demineralized enamel exposed to GSE (×1000 magnification), there are coating depositions of some insoluble complexes on the enamel surface. The reaction products of GSE are seen as amorphous clumps. Spherical globular agglomerates were observed on the surface of the enamel, with varying sizes from place to place. Figure 3Bpresents the previous photomicrograph at ×2500 magnification.


Remineralization of artificial caries in primary teeth by grape seed extract: an in vitro study.

Mirkarimi M, Eskandarion S, Bargrizan M, Delazar A, Kharazifard MJ - J Dent Res Dent Clin Dent Prospects (2013)

© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3935551&req=5

Mentions: Figure 1shows the SEM photomicrograph of sound enamel (×1000 magnification), revealing an orderly smooth appearance. There are also some spherical particles on the surface.Figure 2shows the photomicrograph of demineralized enamel (×1000 magnification) before treatment; the enamel surface is rough and disorganized with significant porosities. In Figure 3Ashowing the demineralized enamel exposed to GSE (×1000 magnification), there are coating depositions of some insoluble complexes on the enamel surface. The reaction products of GSE are seen as amorphous clumps. Spherical globular agglomerates were observed on the surface of the enamel, with varying sizes from place to place. Figure 3Bpresents the previous photomicrograph at ×2500 magnification.

Bottom Line: Results.Grape seed extract significantly increased the micro-hardness of the lesions (P=0.03).Conclusion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Assistant Professor, Department of Pediatric dentistry, Children and Adolescent Health Research Center, Zahedan University of Medical Sciences, Za-hedan, Iran.

ABSTRACT
Background and aims. Promoting remineralization is the ultimate goal of clinical prevention of caries lesion. The present in vitro study aimed to investigate the effect of grape seed extract (GSE) on artificial enamel caries in primary human teeth. Materials and methods. Seventeen human sound primary incisors were sectioned mesiodistally. The tooth slices were placed in a demineralizing solution for 96 hours at 37ºC and 50% relative humidity to create lesions. The demineralized fragments of each tooth were randomly divided into two case (immersed in GSE solution in phosphate buffer for 8 days) and control (immersed in distilled water) groups. The samples were subsequently evaluated using a scanning electron microscope and a micro-hardness tester. Data were analyzed using independent t-test. Results. The mean ± SD micro-hardness values for the case and control groups were 358.6±83.42 and 296.51± 69.41, respectively. Grape seed extract significantly increased the micro-hardness of the lesions (P=0.03). The morphology of GSE treated enamel was clearly different from that in the control group, and there were deposits of scaffolding insoluble complexes on the enamel surface. Conclusion. GSE enhanced the remineralization process of artificial enamel lesions of primary teeth, and thus, might be considered an effective natural agent in non-invasive dentistry.

No MeSH data available.


Related in: MedlinePlus